<abstract xmlns="http://www.w3.org/1999/xhtml">The aim of our study was to stablish the possible alternations in fatty acid composition of brain phospholipids in rats on a standard and high-fat diet supplemented with buckwheat leaf and flower mixture (BLF) and subsequent possible beneficial effects of BLF. Four months old male Wistar rats were randomly divided into five experimental groups fed a standard diet, standard diet supplemented with 5% BLF, high-fat diet, high-fat diet with full-period (13 weeks) of 5% BLF supplementation and high-fat diet with partial-period (7 weeks) of 5% BLF supplementation. Gas-liquid chromatography was performed to analyze the fatty acids in hexane lipid extracts of whole rat brains.Supplementation with BLF did not induce significant changes in fatty acid composition of whole brain phospholipids in rats fed the standard diet. In rats on high-fat diet concomitant (full-period) BLF supplementation increased eicosapentaenoic acid (20:5n-3, EPA), total n-6 and n-6/n-3 ratio, and decreased the percentage of oleic acid (18:1n-9) and estimated activity of Δ-9 desaturase. When BLF application was postponed (partial-period) in the case of developed hyperlipidemia, a decrease of stearic acid (18:0) accompanied with an increased estimated Δ-9 desaturase activity was observed. Regardless of BLF supplementation all high-fat diet-fed groups showed an elevated percentage of linoleic acid (18:2n-6, LA) and a reduced estimated Δ-6 desaturase activity.BLF contributes to the maintenance of stable fatty acid composition of brain phospholipids and supports normal brain function in high-fat diet rats, with more positive effects when BLF was applied before hyperlipidemia developed. This could be the mode of buckwheat health beneficial effects on the brain.</abstract>

The aim of our study was to stablish the possible alternations in fatty acid composition of brain phospholipids in rats on a standard and high-fat diet supplemented with buckwheat leaf and flower mixture (BLF) and subsequent possible beneficial effects of BLF. Four months old male Wistar rats were randomly divided into five experimental groups fed a standard diet, standard diet supplemented with 5% BLF, high-fat diet, high-fat diet with full-period (13 weeks) of 5% BLF supplementation and high-fat diet with partial-period (7 weeks) of 5% BLF supplementation. Gas-liquid chromatography was performed to analyze the fatty acids in hexane lipid extracts of whole rat brains.Supplementation with BLF did not induce significant changes in fatty acid composition of whole brain phospholipids in rats fed the standard diet. In rats on high-fat diet concomitant (full-period) BLF supplementation increased eicosapentaenoic acid (20:5n-3, EPA), total n-6 and n-6/n-3 ratio, and decreased the percentage of oleic acid (18:1n-9) and estimated activity of Δ-9 desaturase. When BLF application was postponed (partial-period) in the case of developed hyperlipidemia, a decrease of stearic acid (18:0) accompanied with an increased estimated Δ-9 desaturase activity was observed. Regardless of BLF supplementation all high-fat diet-fed groups showed an elevated percentage of linoleic acid (18:2n-6, LA) and a reduced estimated Δ-6 desaturase activity.BLF contributes to the maintenance of stable fatty acid composition of brain phospholipids and supports normal brain function in high-fat diet rats, with more positive effects when BLF was applied before hyperlipidemia developed. This could be the mode of buckwheat health beneficial effects on the brain.

Effects Of Dietary Supplementation With A Mixture Of Buckwheat Leaf And Flower On Fatty Acid Composition Of Rat Brain Phospholipids

Centre of Research Excellence in Nutrition and Metabolism, Institute for Medical Research, University of Belgrade, Tadeusa Koscuska 1, 11000 Belgrade, Serbia

Institute of Forensic Medicine, Clinical Center Vojvodina, Hajduk Veljkova 7-9, 21000 Novi Sad, Serbia

Department of Pharmacy, Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, 21000 Novi Sad, Serbia

Institute for Food Technology, University of Novi Sad, Bulevar cara Lazara 1; 21000 Novi Sad, Serbia

Acta Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

EFEKAT DIJETARNE SUPLEMENTACIJE SA MEŠAVINOM LISTA I CVETA HELJDE NA MASNOKISELINSKI PROFIL FOSFOLIPIDA MOZGA PACOVA

The aim of our study was to stablish the possible alternations in fatty acid composition of brain phospholipids in rats on a standard and high-fat diet...

Effects Of Dietary Supplementation With A Mixture Of Buckwheat Leaf And Flower On Fatty Acid Composition Of Rat Brain Phospholipids

Publicado en línea: 30 sept 2015

Páginas: 390 - 403

Recibido: 17 dic 2014

Aceptado: 30 abr 2015

DOI: https://doi.org/10.1515/acve-2015-0032

Palabras clave
buckwheat leaf and flower mixture, high-fat diet, brain phospholipids, fatty acids

© Petrović Snježana et al.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Effects Of Dietary Supplementation With A Mixture Of Buckwheat Leaf And Flower On Fatty Acid Composition Of Rat Brain Phospholipids

Publicado en línea: 30 sept 2015

Páginas: 390 - 403

Recibido: 17 dic 2014

Aceptado: 30 abr 2015

DOI: https://doi.org/10.1515/acve-2015-0032

Palabras clavebuckwheat leaf and flower mixture, high-fat diet, brain phospholipids, fatty acids

© Petrović Snježana et al.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Palabras clave
buckwheat leaf and flower mixture, high-fat diet, brain phospholipids, fatty acids