<abstract xmlns="http://www.w3.org/1999/xhtml"> The present study was designed to identify the chemical constituents of the methanolic extract of Curcuma longa L. rhizomes and their inhibitory effect on a hepatoma cell line. The methanolic extract was subjected to GC-MS analysis to identify the volatile constituents and the other part of the same extract was subjected to liquid column chromatographic separation to isolate curcumin. The inhibition of cell growth in the hepatoma cell line and the cytopathological changes were studied. GC-MS analysis showed the presence of fifty compounds in the methanolic extract of C. longa. The major compounds were ar-turmerone (20.50 %), β-sesquiphellandrene (5.20 %) and curcumenol (5.11 %). Curcumin was identified using IR, 1H and 13C NMR. The inhibition of cell growth by curcumin (IC50 = 41.69 ± 2.87 μg mL-1) was much more effective than that of methanolic extract (IC50 = 196.12 ± 5.25 μg mL-1). Degenerative and apoptotic changes were more evident in curcumin- treated hepatoma cells than in those treated with the methanol extract. Antitumor potential of the methanolic extract may be attributed to the presence of sesquiterpenes and phenolic constituents including curcumin (0.051 %, 511.39 μg g-1 dried methanol extract) in C. longa rhizomes.</abstract>

The present study was designed to identify the chemical constituents of the methanolic extract of Curcuma longa L. rhizomes and their inhibitory effect on a hepatoma cell line. The methanolic extract was subjected to GC-MS analysis to identify the volatile constituents and the other part of the same extract was subjected to liquid column chromatographic separation to isolate curcumin. The inhibition of cell growth in the hepatoma cell line and the cytopathological changes were studied. GC-MS analysis showed the presence of fifty compounds in the methanolic extract of C. longa. The major compounds were ar-turmerone (20.50 %), β-sesquiphellandrene (5.20 %) and curcumenol (5.11 %). Curcumin was identified using IR, 1H and 13C NMR. The inhibition of cell growth by curcumin (IC50 = 41.69 ± 2.87 μg mL-1) was much more effective than that of methanolic extract (IC50 = 196.12 ± 5.25 μg mL-1). Degenerative and apoptotic changes were more evident in curcumin- treated hepatoma cells than in those treated with the methanol extract. Antitumor potential of the methanolic extract may be attributed to the presence of sesquiterpenes and phenolic constituents including curcumin (0.051 %, 511.39 μg g-1 dried methanol extract) in C. longa rhizomes.

Bioactive chemical constituents of Curcuma longa L. rhizomes extract inhibit the growth of human hepatoma cell line (HepG2)

Medicinal Chemistry, Theodor Bilharz Research Institute Kornaish El-Nile 12661 Warrak El-Hadar Imbaba (P.O. 30), Giza, Egypt

Immunology, Theodor Bilharz Research Institute Kornaish El-Nile 12661 Warrak El-Hadar Imbaba (P.O. 30), Giza, Egypt

Pathology, Theodor Bilharz Research Institute Kornaish El-Nile 12661 Warrak El-Hadar Imbaba (P.O. 30), Giza, Egypt

Environmental Research Laboratories Theodor Bilharz Research Institute Kornaish El-Nile 12661 Warrak El-Hadar Imbaba (P.O. 30), Giza, Egypt

Acta Pharmaceutica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

The present study was designed to identify the chemical constituents of the methanolic extract of Curcuma longa L. rhizomes and their inhibitory effect on a...

Bioactive chemical constituents of Curcuma longa L. rhizomes extract inhibit the growth of human hepatoma cell line (HepG2)

Publicado en línea: 29 jun 2016

Páginas: 387 - 398

Aceptado: 21 feb 2016

DOI: https://doi.org/10.1515/acph-2016-0028

Palabras clave
Curcuma longa L., GC-MS analysis, curcumin, anticancer activity

© by Ezzat Abdel-Lateef

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Bioactive chemical constituents of Curcuma longa L. rhizomes extract inhibit the growth of human hepatoma cell line (HepG2)

Publicado en línea: 29 jun 2016

Páginas: 387 - 398

Aceptado: 21 feb 2016

DOI: https://doi.org/10.1515/acph-2016-0028

Palabras claveCurcuma longa L., GC-MS analysis, curcumin, anticancer activity

© by Ezzat Abdel-Lateef

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Palabras clave
Curcuma longa L., GC-MS analysis, curcumin, anticancer activity