<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Quality control of veterinary medicine products containing two different frequently used antibiotics metronidazole and ciprofloxacin hydrochloride, was considered and performed, using modified pharmacopoeial HPLC methods. Three different HPLC systems were used: Varian ProStar, Perkin Elmer Series and UPLC Shimadzu Prominence XR. The chromatographic columns used were LiChropher RP Select B 75 mm × 4 mm with 5 μm particles and Discovery C18 100 mm × 4,6 mm with 5 μm particles. Chromatographic methods used for both analytes were compendial, with minor modifications made for experimental purposes. Minor modifications of the pharmacopoeia prescribed chromatographic conditions, in both cases, led to better chromatographic parameters, good resolution and shorter analysis times. Optimized methods can be used for: determination of metronidazole in gel formulation, for its simultaneous quantification with preservatives present in the formulation and even for identification and quantification of its specified impurity, 2-methyl-5-nitroimidazole; determination of ciprofloxacin hydrochloride in film coated tablets and eye drops and identification and quantification of its specified impurities. These slightly modified and optimized pharmacopoeial methods for quality control of metronidazole and ciprofloxacin dosage forms used in veterinary medicine can be successfully applied in laboratories for quality control of veterinary medicines.</p></abstract>

Quality control of veterinary medicine products containing two different frequently used antibiotics metronidazole and ciprofloxacin hydrochloride, was considered and performed, using modified pharmacopoeial HPLC methods. Three different HPLC systems were used: Varian ProStar, Perkin Elmer Series and UPLC Shimadzu Prominence XR. The chromatographic columns used were LiChropher RP Select B 75 mm × 4 mm with 5 μm particles and Discovery C18 100 mm × 4,6 mm with 5 μm particles. Chromatographic methods used for both analytes were compendial, with minor modifications made for experimental purposes. Minor modifications of the pharmacopoeia prescribed chromatographic conditions, in both cases, led to better chromatographic parameters, good resolution and shorter analysis times. Optimized methods can be used for: determination of metronidazole in gel formulation, for its simultaneous quantification with preservatives present in the formulation and even for identification and quantification of its specified impurity, 2-methyl-5-nitroimidazole; determination of ciprofloxacin hydrochloride in film coated tablets and eye drops and identification and quantification of its specified impurities. These slightly modified and optimized pharmacopoeial methods for quality control of metronidazole and ciprofloxacin dosage forms used in veterinary medicine can be successfully applied in laboratories for quality control of veterinary medicines.

Preliminary Investigation of the Possibility for Implementation of Modified Pharmacopoeial HPLC Methods For Quality Control Of Metronidazole And Ciprofloxacin In Medicinal Products Used In Veterinary Medicine

Pharmaceutical Company Replek Farm Ltd., Quality Control Department st. Kozle 188, 1000 Skopje

Replek Farm Ltd., Quality Control Department st. Kozle 188, Skopje, Republic of Macedonia

Macedonian Veterinary Review

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Quality control of veterinary medicine products containing two different frequently used antibiotics metronidazole and ciprofloxacin hydrochloride, was...

Preliminary Investigation of the Possibility for Implementation of Modified Pharmacopoeial HPLC Methods For Quality Control Of Metronidazole And Ciprofloxacin In Medicinal Products Used In Veterinary Medicine

Publicado en línea: 18 nov 2015

Páginas: 31 - 42

Recibido: 03 jun 2014

Aceptado: 10 nov 2014

DOI: https://doi.org/10.14432/j.macvetrev.2014.11.029

Palabras clave
metronidazole, ciprofloxacin hydrochloride, veterinary medicines, pharmacopoeia, quality control

© 2015

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Preliminary Investigation of the Possibility for Implementation of Modified Pharmacopoeial HPLC Methods For Quality Control Of Metronidazole And Ciprofloxacin In Medicinal Products Used In Veterinary Medicine

Publicado en línea: 18 nov 2015

Páginas: 31 - 42

Recibido: 03 jun 2014

Aceptado: 10 nov 2014

DOI: https://doi.org/10.14432/j.macvetrev.2014.11.029

Palabras clavemetronidazole, ciprofloxacin hydrochloride, veterinary medicines, pharmacopoeia, quality control

© 2015

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Palabras clave
metronidazole, ciprofloxacin hydrochloride, veterinary medicines, pharmacopoeia, quality control