1. bookVolume 22 (2014): Issue 1 (March 2014)
Journal Details
License
Format
Journal
eISSN
2284-5623
ISSN
2284-5623
First Published
08 Aug 2013
Publication timeframe
4 times per year
Languages
English
access type Open Access

Determination of genistein in rat liver and kidney by a HPLC/UV method. Possible extrapolation from animals to humans

Journal Details
License
Format
Journal
eISSN
2284-5623
ISSN
2284-5623
First Published
08 Aug 2013
Publication timeframe
4 times per year
Languages
English
Abstract

A simple and selective method for genistein (GNST) determination in rat liver and kidney was validated in order to study the phytoestrogenic effect of GNST in ovariectomised female Wistar rats. GNST was separated on a Kromasil 100-RP8 column, 150 mm x 4.6 mm, 5 mm equipped with a Kromasil RP 8 precolumn. The mobile phase was 55:45 (v / v) phosphoric acid, 15 mmol in water: methanol at a flow rate of 1.3 ml / min. Luteolin 20 μg / ml in methanol was used as internal standard (IS). The retention time of GNST was tR = 13.22 min and tR =11.60 min for the IS. Calibration curves in the range 40-400 μg GNST/100g liver and 20-200 μg GNST/100g kidney presented a coefficient of determination higher than 0.99. The method developed presented a good precision and accuracy at the lower limit of quantification LLOQ. 10 white Wistar female rats, 8 weeks of age were treated s.c. with 10 mg GNST/kg bw/day for 8 weeks, while a group of 10 animals were used as controls. The values obtained for GNST in the liver were 192.12 ± 53.46 μg/100g and 74.51 ± 12.77 μg/100g in kidney samples.

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1. McCarty MF. Isoflavones made simple. Genistein’s agonist activity for the beta-type estrogen receptor mediates their health benefits. Med Hypotheses. 2006;66(6):1093-114. DOI: 10.1016/j. mehy.2004.11.046Search in Google Scholar

2. Cederroth CR, Nef S. Soy, phytoestrogens and metabolism: A review. Molecular and Cellular Endocrinology. 2009 May 25;304(1-2):30-42. DOI: 10.1016/j. mce.2009.02.027Search in Google Scholar

3. Newbold RR, Banks EP, Bullock B, Jefferson WN. Uterine adenocarcinoma in mice treated neonatally with genistein. Cancer Res. 2001 Jun 1;61(11):4325-8.Search in Google Scholar

4. Weber KS, Setchell KD, Stocco DM, Lephart ED. Dietary soy-phytoestrogens decrease testosterone levels and prostate weight without altering LH, prostate 5alpha- reductase or testicular steroidogenic acute regulatory peptide levels in adult male Sprague-Dawley rats. J Endocrinol. 2001 Sep;170(3):591-9. DOI: 10.1677/ joe.0.170059110.1677/joe.0.170059111524239Search in Google Scholar

5. Coldham NG, Sauer MJ. Pharmacokinetics of [14C] genistein in the rat: gender-related differences, potential mechanisms of biological action and implications for human health. Toxicol Appl Pharmacol. 2000;164(2):206-15. DOI: 10.1006/taap.2000.890210.1006/taap.2000.890210764634Search in Google Scholar

6. Grace PB, Mistry NS, Carter MH, Leath AJ, Teale P. High throughput quantification of phytoestrogens in human urine and serum using liquid chromatography / tandem mass spectrometry (LC-MS/MS). J Chromatogr B. 2007;853(1-2):138-46. DOI: 10.1016/j. jchromb.2007.03.011Search in Google Scholar

7. Tamames-Tabara C, Imbuluzqueta E, Campanero MA, Horcajada P, Blanco-Prietoa MJ. A simple and robust high-performance liquid chromatography coupled to a diode-array detector method for the analysis of genistein in mouse tissues. J Chromatogr B. 2013;935:47-53. DOI: 10.1016/j.jchromb.2013.07.02010.1016/j.jchromb.2013.07.02023939202Search in Google Scholar

8. Matthies A, Loh G, Blaut M, Braune A. Daidzein and Genistein Are Converted to Equol and 5-Hydroxy- Equol by Human Intestinal Slackia isoflavoniconvertens in Gnotobiotic Rats. J Nutr. 2012 Jan 1;142(1):40-6. DOI: 10.3945/jn.111.14824710.3945/jn.111.14824722113864Search in Google Scholar

9. Chen C-Y, Bakhiet RM. Age decreased steady-state concentrations of genistein in plasma, liver, and skeletal muscle in Sprague-Dawley rats. Mech Ageing Dev. 2006 Apr;127(4):344-8. DOI: 10.1016/j. mad.2005.12.003Search in Google Scholar

10. Joshi JV, Vaidya RA, Pandey SN, Agashe S, Chandrasekharan S, Menon SK, et al. Plasma levels of genistein following a single dose of soy extract capsule in Indian women. Indian J Med Res. 2007 Apr;125(4):534-41.Search in Google Scholar

11. Tero-Vescan A, Imre S, Vari CE, Dogaru MT. Determination of genistein in plasma by HPLC-UV. Timisoara Medical Journal. 2008;58(Supplement 2):10-4Search in Google Scholar

12. *** International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for human use, Guidance on nonclinical safety studies for the conduct of human clinical trials and marketing authorization for pharmaceuticals, ICH Harmonised Tripartite Guideline M3(R2), 2009Search in Google Scholar

13. *** ICH Common Technical Document (CTD), vol 2B - Notice to Applicants, Medicinal products for Human use (http://ec.europa.eu/health/files/eudralex/vol-2/b/update_200805/ctd_05-2008_en.pdf)Search in Google Scholar

14. **** „Order no. 244/401/2005 of the Minister of Agriculture, Forestry and Rural Development and the Minister of Health of Romania”Search in Google Scholar

15. Bursztyka J, Perdu E, Tulliez J, Debrauwer L, Delous G, Canlet C, et al. Comparison of genistein metabolism in rats and humans using liver microsomes and hepatocytes. Food and Chemical Toxicology. 2008 Mar;46(3):939-48. DOI: 10.1016/j.fct.2007.10.02310.1016/j.fct.2007.10.02318063284Search in Google Scholar

16. Moon YJ, Wang X, Morris ME. Dietary flavonoids: Effects on xenobiotic and carcinogen metabolism. Toxicology in Vitro. 2006 Mar;20(2):187-210. DOI: 10.1016/j.tiv.2005.06.04810.1016/j.tiv.2005.06.04816289744Search in Google Scholar

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