1. bookVolume 21 (2013): Issue 3 (September 2013)
Journal Details
License
Format
Journal
eISSN
2284-5623
ISSN
2284-5623
First Published
08 Aug 2013
Publication timeframe
4 times per year
Languages
English
access type Open Access

Molecular detection of rifampicin resistance in multidrug-resistant Mycobacterium tuberculosis strains from North-Eastern Romania

Journal Details
License
Format
Journal
eISSN
2284-5623
ISSN
2284-5623
First Published
08 Aug 2013
Publication timeframe
4 times per year
Languages
English
Abstract

Introduction: Multidrug-resistant tuberculosis (MDR-TB) represents an important public health problem. Rapid molecular methods detect the mutations responsible for drug- resistance and can shorten the time required for diagnosis and initiation of appropriate therapy. The objectives of our study were to optimize a multiplex allele-specific PCR technique (MAS-PCR) to detect rpoB mutations and to specify the type and frequency of these mutations. Material and methods: We have tested 47 non-duplicate MDR-TB, including extensively drugresistant tuberculosis (XDR-TB) strains, identified during 2007-2012, using MAS-PCR for detection of mutations in rpoB codons 531, 526 and 516. Results: The most common mutation was located in codon 531 (63.82%), while only 12.76% and 10.63% of the strains showed mutations in codon 516 and 526, respectively; for six strains we have not identified mutation in the targeted codons. Conclusion: MAS-PCR revealed the mutations prevalent in our region, with good sensitivity (87.2%), suggesting the usefulness of this test in the rapid diagnosis of MDR-TB

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1. Gupta R, Espinal MA, Raviglione MC. Tuberculosis as a major global health problem in the 21st century: a WHO perspective. Semin Respir Crit Care Med. 2004;25(3):245-53.10.1055/s-2004-829520Search in Google Scholar

2. WHO: Global tuberculosis report 2012. http://www.who.int/tb/data.Search in Google Scholar

3. Victor TC, van Helden PD, Warren R. Prediction of drug resistance in M. tuberculosis: molecular mechanisms, tools, and applications. IUBMB Life. 2002;53(4-5):231-7.10.1080/15216540212642Search in Google Scholar

4. Ramaswamy S, Musser JM. Molecular genetic basis of antimicrobial agent resistance in Mycobacterium tuberculosis: 1998 update. Tuber Lung Dis. 1998;79(1):3-29.10.1054/tuld.1998.0002Search in Google Scholar

5. Mokrousov I, Otten T, Vyshnevskiy B, Narvskaya O. Allele-specific rpoB PCR assays for detection of rifampinresistant Mycobacterium tuberculosis in sputum smears. Antimicrob Agents Chemother. 2003;47(7):2231-5.10.1128/AAC.47.7.2231-2235.2003Search in Google Scholar

6. Parsons LM, Somoskövi A, Gutierrez C, Lee E, Paramasivan CN, Abimiku A et al. Laboratory diagnosis of tuberculosis in resource-poor countries: challenges and opportunities.Search in Google Scholar

J Clin Microbiol Rev. 2011;24(2):314-50.10.1128/CMR.00059-10Search in Google Scholar

7. Palomino JC. Molecular detection, identification and drug resistance detection in Mycobacterium tuberculosis. FEMS Immunol Med Microbiol. 2009;56(2):103-11.10.1111/j.1574-695X.2009.00555.xSearch in Google Scholar

8. Gillespie SH. Evolution of Drug Resistance in Mycobacterium tuberculosis: Clinical and Molecular Perspective. Antimicrob Agents Chemother. 2002; 46(2): 267-74.10.1128/AAC.46.2.267-274.2002Search in Google Scholar

9. Telenti A, Imboden P, Marchesi F, Lowrie D, Cole S, Colston MJ et al. Detection of rifampicin-resistance mutations in Mycobacterium tuberculosis. Lancet. 1993;341(8846):647-50.10.1016/0140-6736(93)90417-FSearch in Google Scholar

10. Cavusoglu C, Hilmioglu S, Guneri S, Bilgic A. Characterization of rpoB mutations in rifampin-resistant clinical isolates of Mycobacterium tuberculosis from Turkey by DNA sequencing and line probe assay. J Clin Microbiol. 2002;40(12):4435-8.10.1128/JCM.40.12.4435-4438.200215465112454132Search in Google Scholar

11. G. Canetti, S. Froman, J. Grosset, P. Hauduroy. Mycobacteria: Laboratory methods for testing drug sensitivity and resistance. Bull World Health Organ. 1963;29:565-8.Search in Google Scholar

12. G. Canetti, W. Fox, A. Khomenko, H.T. Mahler, N.K. Menon, D.A. Mitchison et al. Advances in techniques of testing mycobacterial drug sensitivity, and the use of sensitivity tests in tuberculosis control programmes. Bull World Health Organ. 1969;41:21-43.Search in Google Scholar

13. Kim SJ. Drug-susceptibility testing in tuberculosis: Methods and reliability of results. Eur Respir J. 2005;25:564-9.10.1183/09031936.05.0011130415738303Search in Google Scholar

14. Cavusoglu C, Turhan A, Akinci P, Soyler I. Evalua- tion of the Genotype MTBDR assay for rapid detection of rifampin and isoniazid resistance in Mycobacterium tuberculosis isolates. J Clin Microbiol. 2006;44(7):2338-42.10.1128/JCM.00425-06Search in Google Scholar

15. Vitoria M, Granich R, Gilks CF, Gunneberg C, Hosseini M, Were Wetal. The Global Fight Against HIV/AIDS, Tuberculosis, and Malaria Current Status and Future Perspectives. Am J Clin Pathol.2009;131:844-8.10.1309/AJCP5XHDB1PNAEYTSearch in Google Scholar

16. Ghid Metodologic De Implementare A Programului Naţional De Control Al Tuberculozei 2007-2011, Institutul Naţional De Pneumologie “Marius Nasta” Bucureşti, 2007, http://www.srp.ro/Programul-Nationalde-Control-al-Tuberculozei-118.htmSearch in Google Scholar

17. Mokrousov I, Filliol I, Legrand E, Sola C, Otten T, Vyshnevskaya E et al. Molecular characterization of multiple- drug-resistant Mycobacterium tuberculosis isolates from northwestern Russia and analysis of rifampin resistance using RNA/RNA mismatch analysis as compared to the line probe assay and sequencing of the rpoB gene. Res Microbiol. 2002;153(4):213-9.10.1016/S0923-2508(02)01311-6Search in Google Scholar

18. Generozov EV, Akopian TA, Govorun VM, Chernousova LN, Larionova EE, Savinkova SN et al. Molecular characteristics of multiresistant clinical strains of Mycobacterium tuberculosis isolated in Russia. Mol Gen Mikrobiol Virusol. 2000;(1):11-7. RussianSearch in Google Scholar

19. Nikolayevsky V, Brown T, Balabanova Y, Ruddy M, Fedorin I, Drobniewski F. Detection of mutations associated with isoniazid and rifampin resistance in Mycobacterium tuberculosis isolates from Samara Region, Russian Federation. J Clin Microbiol. 2004;42(10):4498-502.10.1128/JCM.42.10.4498-4502.200452229015472300Search in Google Scholar

20. Toungoussova OS, Sandven P, Mariandyshev AO, Nizovtseva NI, Bjune G, Caugant DA. Spread of drug-resistant Mycobacterium tuberculosis strains of the Beijing genotype in the Archangel Oblast, Russia. J Clin Microbiol. 2002;40(6):1930-7.10.1128/JCM.40.6.1930-1937.200213082112037045Search in Google Scholar

21. Huang WL, Chen HY, Kuo YM, Jou R. Performance assessment of the GenoType MTBDRplus test and DNA sequencing in detection of multidrug-resistant Mycobacterium tuberculosis. J Clin Microbiol. 2009;47(8):2520-4.10.1128/JCM.02499-08272563619494067Search in Google Scholar

22. Barnard M, Albert H, Coetzee G, O'Brien R, Bosman ME. Rapid molecular screening for multidrug-resistant tuberculosis in a high-volume public health laboratory in South Africa. Am J Respir Crit Care Med. 2008;177(7):787-92.10.1164/rccm.200709-1436OC18202343Search in Google Scholar

23. Hristea A, Otelea D, Paraschiv S, Macri A, Baicus C, Moldovan O et al. Detection of Mycobacterium tuberculosis resistance mutations to rifampin and isoniazid by realtime PCR. Indian J Med Microbiol. 2010;28:211-6.10.4103/0255-0857.6647420644308Search in Google Scholar

24. Miotto P, Piana F, Penati V, Canducci F, Migliori GB, Cirillo DM. Use of genotype MTBDR assay for molecular detection of rifampin and isoniazid resistance in Mycobacterium tuberculosis clinical strains isolated in Italy. J Clin Microbiol. 2006;44(7):2485-91.10.1128/JCM.00083-06148949716825369Search in Google Scholar

25. Valcheva V, Mokrousov I, Narvskaya O, Rastogi N, Markova N. Molecular snapshot of drug-resistant and drug-susceptible Mycobacterium tuberculosis strains circulating in Bulgaria. Infect Genet Evol. 2008;8:657-63.10.1016/j.meegid.2008.06.00618657631Search in Google Scholar

26. Dymova MA, Liashenko OO, Poteiko PI, Krutko VS, Khrapov EA, Filipenko ML. Genetic variation of Mycobacterium tuberculosis circulating in Kharkiv Oblast, Ukraine. BMC Infect Dis. 2011;11:77.10.1186/1471-2334-11-77307965021439097Search in Google Scholar

27. Hillemann D, Weizenegger M, Kubica T, Richter E, Niemann S. Use of the genotype MTBDR assay for rapid detection of rifampin and isoniazid resistance in Mycobacterium tuberculosis complex isolates. J Clin Microbiol. 2005;43(8):3699-703.10.1128/JCM.43.8.3699-3703.2005123390316081898Search in Google Scholar

28. Bártfai Z, Somoskövi A, Ködmön C, Szabó N, Puskás E, Kosztolányi L et al. Molecular characterization of rifampin-resistant isolates of Mycobacterium tuberculosis from Hungary by DNA sequencing and the line probe assay. J Clin Microbiol. 2001;39(10):3736-9.10.1128/JCM.39.10.3736-3739.20018842111574605Search in Google Scholar

29. Crudu V, Stratan E, Romancenco E, Allerheiligen V, Hillemann A, Moraru N. First evaluation of an improved assay for molecular genetic detection of tuberculosis as well as rifampin and isoniazid resistances. J Clin Microbiol. 2012;50(4):1264-9.10.1128/JCM.05903-11331852822301019Search in Google Scholar

30. Rosales-Klintz S, Jureen P, Zalutskayae A, Skrahina A, Xu B, Yi Hu Y et al. Drug resistance-related mutations in multidrug-resistant Mycobacterium tuberculosis isolates from diverse geographical regions. Int J Mycobacteriol. 2012;1(3):124-30. 10.1016/j.ijmyco.2012.08.00126787207Search in Google Scholar

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