<abstract xmlns="http://www.w3.org/1999/xhtml"><sec id="j_1905-7415.1102.546_s_001_w2aab3b7c12b1b6b1aab1c15b1Aa"><h3>Background</h3><p>Korean radish (<italic>Raphanus sativus</italic> L.) is used in <italic>kimchi</italic>, a staple of Korean cuisine. A taffy, <italic>mu yeot</italic>, that includes mainly radish together with other herbs (Bulbus Allii Sativi, Fructus Zizyphi, Semen Zizyphi, Rhizoma Zingberis, and Radix Glycyrrihizae) is used traditionally in Korea to improve pulmonary symptoms. However, to our knowledge, these effects of the taffy have not yet been studied systematically.</p></sec><sec id="j_1905-7415.1102.546_s_002_w2aab3b7c12b1b6b1aab1c15b2Aa"><h3>Objectives</h3><p>To elucidate the anti-inflammatory effects of radish taffy on macrophages and a murine model of pulmonary inflammation, and to suggest the most likely candidate for the effects.</p></sec><sec id="j_1905-7415.1102.546_s_003_w2aab3b7c12b1b6b1aab1c15b3Aa"><h3>Methods</h3><p>A macrophage cell line, RAW264.7, was used to examine effects of the radish taffy in vitro. Pulmonary inflammation was assessed 24 h after oral instillation of lipopolysaccharide in mice treated with radish taffy for 10 days. We determined the chemical components of radish taffy quantitatively using tandem HPLC mass spectroscopy.</p></sec><sec id="j_1905-7415.1102.546_s_004_w2aab3b7c12b1b6b1aab1c15b4Aa"><h3>Results</h3><p>Radish taffy had no apparent effect on the RAW264.7 cells. Treatment of the mice with radish taffy significantly reduced the recruitment of neutrophils to the lung, and the level of myeloperoxidase in bronchoalveolar lavage fluid (BALF). ELISAs showed that the treatment significantly decreased the level of IL 6 in BALF induced by LPS, but not the levels of IFNγ, TNFα, IL 10, or IL 12. Nor did the taffy change the levels of those cytokines or NF-κB activation in lung homogenates. HPLC-MS suggested glycyrrhizin as the most likely candidate for the anti-inflammatory effects.</p></sec><sec id="j_1905-7415.1102.546_s_005_w2aab3b7c12b1b6b1aab1c15b5Aa"><h3>Conclusions</h3><p>Radish taffy suppresses neutrophil recruitment to lungs of mice, possibly by reducing IL 6 levels, which may lead to reduced pulmonary inflammation.</p></sec></abstract>

BackgroundKorean radish (Raphanus sativus L.) is used in kimchi, a staple of Korean cuisine. A taffy, mu yeot, that includes mainly radish together with other herbs (Bulbus Allii Sativi, Fructus Zizyphi, Semen Zizyphi, Rhizoma Zingberis, and Radix Glycyrrihizae) is used traditionally in Korea to improve pulmonary symptoms. However, to our knowledge, these effects of the taffy have not yet been studied systematically.ObjectivesTo elucidate the anti-inflammatory effects of radish taffy on macrophages and a murine model of pulmonary inflammation, and to suggest the most likely candidate for the effects.MethodsA macrophage cell line, RAW264.7, was used to examine effects of the radish taffy in vitro. Pulmonary inflammation was assessed 24 h after oral instillation of lipopolysaccharide in mice treated with radish taffy for 10 days. We determined the chemical components of radish taffy quantitatively using tandem HPLC mass spectroscopy.ResultsRadish taffy had no apparent effect on the RAW264.7 cells. Treatment of the mice with radish taffy significantly reduced the recruitment of neutrophils to the lung, and the level of myeloperoxidase in bronchoalveolar lavage fluid (BALF). ELISAs showed that the treatment significantly decreased the level of IL 6 in BALF induced by LPS, but not the levels of IFNγ, TNFα, IL 10, or IL 12. Nor did the taffy change the levels of those cytokines or NF-κB activation in lung homogenates. HPLC-MS suggested glycyrrhizin as the most likely candidate for the anti-inflammatory effects.ConclusionsRadish taffy suppresses neutrophil recruitment to lungs of mice, possibly by reducing IL 6 levels, which may lead to reduced pulmonary inflammation.

Anti-inflammatory effect of the taffy <italic>mu yeot</italic>, made from the Korean radish <italic>Raphanus sativus</italic> L. in a lipopolysaccharide-induced murine model of pulmonary inflammation

Department of Alternative Medicine, College of Medical Sciences

Asian Biomedicine

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Anti-inflammatory effect of the taffy , made from the Korean radish  L. in a lipopolysaccharide-induced murine model of pulmonary inflammation

BackgroundKorean radish (Raphanus sativus L.) is used in kimchi, a staple of Korean cuisine. A taffy, mu yeot, that includes mainly radish together with other...

Anti-inflammatory effect of the taffy mu yeot, made from the Korean radish Raphanus sativus L. in a lipopolysaccharide-induced murine model of pulmonary inflammation

Article Category: Original Article

Online veröffentlicht: 31. Aug. 2017

Seitenbereich: 145 - 156

DOI: https://doi.org/10.5372/1905-7415.1102.546

Schlüsselwörter
IL 6, lipopolysaccharide, neutrophil, pulmonary inflammation, radish taffy

© 2017 Dan-Hee Kim, Sang-Yun Nam

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Anti-inflammatory effect of the taffy mu yeot, made from the Korean radish Raphanus sativus L. in a lipopolysaccharide-induced murine model of pulmonary inflammation

Article Category: Original Article

Online veröffentlicht: 31. Aug. 2017

Seitenbereich: 145 - 156

DOI: https://doi.org/10.5372/1905-7415.1102.546

SchlüsselwörterIL 6, lipopolysaccharide, neutrophil, pulmonary inflammation, radish taffy

© 2017 Dan-Hee Kim, Sang-Yun Nam

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Schlüsselwörter
IL 6, lipopolysaccharide, neutrophil, pulmonary inflammation, radish taffy