<abstract xmlns="http://www.w3.org/1999/xhtml"><sec id="j_1905-7415.0901.365_s_101_w2aab3b7b5b1b6b1aab1c16b1Aa"><h3>Background</h3><p>Monounsaturated fat (MUFA) also has glucose-lowering action, but its effect on ketone bodies is unknown.</p></sec><sec id="j_1905-7415.0901.365_s_105_w2aab3b7b5b1b6b1aab1c16b2Aa"><h3>Objectives</h3><p>To examine the effects of high-MUFA diet alone or in combination with exercise training, which can improve glucose and ketone body metabolism, in a rat model of diabetes.</p></sec><sec id="j_1905-7415.0901.365_s_102_w2aab3b7b5b1b6b1aab1c16b3Aa"><h3>Methods</h3><p>Wistar rats were administered streptozotocin to induce diabetes and then randomly divided into five groups: sedentary rats fed a regular diet (1), a high-saturated-fat diet (2), a high-MUFA diet (3); and exercisetrained rats fed a regular diet (4), and a high-MUFA diet (5). Training was by a treadmill twice daily, 5 days/week. At 12 weeks, glucose, glycated hemoglobin (HbA<sub>1c</sub>), insulin, nonesterified fatty acids (NEFA), and β-hydroxybutyrate levels were measured in cardiac blood. Activity of the overall ketone synthesis pathway was determined in liver and 3-ketoacyl-CoA transferase activity determined in gastrocnemius muscle.</p></sec><sec id="j_1905-7415.0901.365_s_103_w2aab3b7b5b1b6b1aab1c16b4Aa"><h3>Results</h3><p>A high-MUFA diet tended to lower plasma glucose without affecting other biochemical variables. Training did not change glucose metabolism, but significantly reduced serum NEFA. Only the high-MUFA diet plus training significantly decreased HbA<sub>1c</sub> levels. Hepatic ketone synthesis was decreased and 3-ketoacyl-CoA transferase activity was increased by training alone or in combination with a high-MUFA diet. Changes in NEFA, β-hydroxybutyrate, and the enzymatic activities in response to training plus a high-MUFA diet were comparable to those caused by training alone.</p></sec><sec id="j_1905-7415.0901.365_s_104_w2aab3b7b5b1b6b1aab1c16b5Aa"><h3>Conclusion</h3><p>A high-MUFA diet alone does not alter ketone body metabolism. Combination of a MUFA-rich diet and exercise training is more effective than either MUFA or exercise alone for lowering HbA<sub>1c</sub>.</p></sec></abstract>

BackgroundMonounsaturated fat (MUFA) also has glucose-lowering action, but its effect on ketone bodies is unknown.ObjectivesTo examine the effects of high-MUFA diet alone or in combination with exercise training, which can improve glucose and ketone body metabolism, in a rat model of diabetes.MethodsWistar rats were administered streptozotocin to induce diabetes and then randomly divided into five groups: sedentary rats fed a regular diet (1), a high-saturated-fat diet (2), a high-MUFA diet (3); and exercisetrained rats fed a regular diet (4), and a high-MUFA diet (5). Training was by a treadmill twice daily, 5 days/week. At 12 weeks, glucose, glycated hemoglobin (HbA1c), insulin, nonesterified fatty acids (NEFA), and β-hydroxybutyrate levels were measured in cardiac blood. Activity of the overall ketone synthesis pathway was determined in liver and 3-ketoacyl-CoA transferase activity determined in gastrocnemius muscle.ResultsA high-MUFA diet tended to lower plasma glucose without affecting other biochemical variables. Training did not change glucose metabolism, but significantly reduced serum NEFA. Only the high-MUFA diet plus training significantly decreased HbA1c levels. Hepatic ketone synthesis was decreased and 3-ketoacyl-CoA transferase activity was increased by training alone or in combination with a high-MUFA diet. Changes in NEFA, β-hydroxybutyrate, and the enzymatic activities in response to training plus a high-MUFA diet were comparable to those caused by training alone.ConclusionA high-MUFA diet alone does not alter ketone body metabolism. Combination of a MUFA-rich diet and exercise training is more effective than either MUFA or exercise alone for lowering HbA1c.

A high-MUFA diet alone does not affect ketone body metabolism, but reduces glycated hemoglobin when combined with exercise training in diabetic rats

Department of Physiology, Faculty of Medicine

Department of Biochemistry, Faculty of Medicine

Asian Biomedicine

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

BackgroundMonounsaturated fat (MUFA) also has glucose-lowering action, but its effect on ketone bodies is unknown.ObjectivesTo examine the effects of high-MUFA...

A high-MUFA diet alone does not affect ketone body metabolism, but reduces glycated hemoglobin when combined with exercise training in diabetic rats

Article Category: Original article

Online veröffentlicht: 31. Jan. 2017

Seitenbereich: 31 - 40

DOI: https://doi.org/10.5372/1905-7415.0901.365

Schlüsselwörter
Beta-hydroxybutyric acid, diabetes mellitus, exercise training, glycated hemoglobin, hepatic ketone body synthesis, ketoacyl-CoA transferase, monounsaturated fatty acid, non-esterified fatty acid, peripheral ketone body utilization

© 2017 Juraiporn Somboonwong, Khunkhong Huchaiyaphum, Onanong Kulaputana, Phisit Prapunwattana

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

A high-MUFA diet alone does not affect ketone body metabolism, but reduces glycated hemoglobin when combined with exercise training in diabetic rats

Article Category: Original article

Online veröffentlicht: 31. Jan. 2017

Seitenbereich: 31 - 40

DOI: https://doi.org/10.5372/1905-7415.0901.365

SchlüsselwörterBeta-hydroxybutyric acid, diabetes mellitus, exercise training, glycated hemoglobin, hepatic ketone body synthesis, ketoacyl-CoA transferase, monounsaturated fatty acid, non-esterified fatty acid, peripheral ketone body utilization

© 2017 Juraiporn Somboonwong, Khunkhong Huchaiyaphum, Onanong Kulaputana, Phisit Prapunwattana

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Schlüsselwörter
Beta-hydroxybutyric acid, diabetes mellitus, exercise training, glycated hemoglobin, hepatic ketone body synthesis, ketoacyl-CoA transferase, monounsaturated fatty acid, non-esterified fatty acid, peripheral ketone body utilization