<abstract xmlns="http://www.w3.org/1999/xhtml"> Background: As part of their innate immune response to changes in the central nervous system environment, normally quiescent microglia become activated and increase expression of pattern recognition receptors, scavenger receptors, and production of inflammatory cytokines, proteinases, reactive oxygen species (ROS), and free radicals. These molecules have been implicated in the pathogenesis and progression of several neurodegenerative disorders including Alzheimer disease (AD).Objective: We compared patterns of microglial innate immune responses elicited by nonfibrillar amyloid β peptide (nfAβ1-42) to those elicited by lipopolysaccharide (LPS).Methods: Murine BV-2 microglial cells were exposed to either nfAβ1-42 or LPS for 12 h. Then, total RNA from each condition was isolated and expression levels of Toll-like receptor (TLR)-4, scavenger receptor class A (SRMARCO) and class B (SR-BI), CD36, and matrix metalloproteinase (MMP)-9 were determined by reverse transcription-quantitative real-time polymerase chain reaction. The amount of hydrogen peroxide (H2O2) and nitric oxide (NO) in the cell-free supernatant at 24 h were determined using 10-acetyl-3,7-dihydroxyphenoxazine (Amplex Red) and Griess reagent, respectively.Results: nfAβ1-42 and LPS significantly increased expression of TLR-4, SR-MARCO, CD36, and MMP-9 and production of H2O2 and NO in BV-2 microglial cells compared with that of unstimulated cells. However, expression of SR-BI was significantly induced only when the cells were exposed to nfAβ1-42.Conclusion: These findings indicate that pattern of microglial innate immune responses elicited by nfAβ1-42 overlap with that elicited by LPS and suggest a specific role of microglial SR-BI expression in AD pathogenesis.</abstract>

Background: As part of their innate immune response to changes in the central nervous system environment, normally quiescent microglia become activated and increase expression of pattern recognition receptors, scavenger receptors, and production of inflammatory cytokines, proteinases, reactive oxygen species (ROS), and free radicals. These molecules have been implicated in the pathogenesis and progression of several neurodegenerative disorders including Alzheimer disease (AD).Objective: We compared patterns of microglial innate immune responses elicited by nonfibrillar amyloid β peptide (nfAβ1-42) to those elicited by lipopolysaccharide (LPS).Methods: Murine BV-2 microglial cells were exposed to either nfAβ1-42 or LPS for 12 h. Then, total RNA from each condition was isolated and expression levels of Toll-like receptor (TLR)-4, scavenger receptor class A (SRMARCO) and class B (SR-BI), CD36, and matrix metalloproteinase (MMP)-9 were determined by reverse transcription-quantitative real-time polymerase chain reaction. The amount of hydrogen peroxide (H2O2) and nitric oxide (NO) in the cell-free supernatant at 24 h were determined using 10-acetyl-3,7-dihydroxyphenoxazine (Amplex Red) and Griess reagent, respectively.Results: nfAβ1-42 and LPS significantly increased expression of TLR-4, SR-MARCO, CD36, and MMP-9 and production of H2O2 and NO in BV-2 microglial cells compared with that of unstimulated cells. However, expression of SR-BI was significantly induced only when the cells were exposed to nfAβ1-42.Conclusion: These findings indicate that pattern of microglial innate immune responses elicited by nfAβ1-42 overlap with that elicited by LPS and suggest a specific role of microglial SR-BI expression in AD pathogenesis.

Original article. Patterns of microglial innate immune responses elicited by amyloid β1–42 and lipopolysaccharide: the similarities of the differences

Department of Anatomy, Faculty of Medicine, Chulalongkorn University, Bangkok 10330,

Asian Biomedicine

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Original article. Patterns of microglial innate immune responses elicited by amyloid β and lipopolysaccharide: the similarities of the differences

Background: As part of their innate immune response to changes in the central nervous system environment, normally quiescent microglia become activated and...

Original article. Patterns of microglial innate immune responses elicited by amyloid β_1–42 and lipopolysaccharide: the similarities of the differences

Online veröffentlicht: 04. Feb. 2017

Seitenbereich: 353 - 359

DOI: https://doi.org/10.5372/1905-7415.0802.299

Schlüsselwörter
Alzheimer disease, innate immunity, matrix metalloproteinase, microglia, scavenger receptors, toll-like receptor

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Original article. Patterns of microglial innate immune responses elicited by amyloid β1–42 and lipopolysaccharide: the similarities of the differences

Online veröffentlicht: 04. Feb. 2017

Seitenbereich: 353 - 359

DOI: https://doi.org/10.5372/1905-7415.0802.299

SchlüsselwörterAlzheimer disease, innate immunity, matrix metalloproteinase, microglia, scavenger receptors, toll-like receptor

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Original article. Patterns of microglial innate immune responses elicited by amyloid β_1–42 and lipopolysaccharide: the similarities of the differences

Schlüsselwörter
Alzheimer disease, innate immunity, matrix metalloproteinase, microglia, scavenger receptors, toll-like receptor