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Developing the Tissue Engineered Heart Valve – a Descriptive Hemodynamic and Ultrasound in Vitro Characterization Study of Heart Valves in a Bioreactor

Ionela Movileanu
Ionela Movileanu
, 
Dan Octavian Nistor
Dan Octavian Nistor
, 
Leslie Sierad
Leslie Sierad
, 
Klara Brinzaniuc
Klara Brinzaniuc
, 
Codrut Oprita
Codrut Oprita
, 
Marius Mihai Harpa
Marius Mihai Harpa
, 
Terezia Preda
Terezia Preda
, 
Ovidiu Cotoi
Ovidiu Cotoi
, 
Hussam AL Hussein
Hussam AL Hussein
, 
Hamida AL Hussein
Hamida AL Hussein
, 
Alexandru Chertes
Alexandru Chertes
, 
Horatiu Suciu
Horatiu Suciu
, 
Agneta Simionescu
Agneta Simionescu
 und 
Dan Simionescu
Dan Simionescu
   | 05. Mai 2022
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Article Category: Original Article
Online veröffentlicht: 05. Mai 2022
Seitenbereich: 555 - 563
DOI: https://doi.org/10.47803/rjc.2021.31.3.555
Schlüsselwörter
© 2021 Ionela Movileanu et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1

Tested valvular models – A – Decellularized porcine aortic valve; B – Decellularized porcine pulmonary valve; C- Biological prosthesis; D – Fresh porcine aortic valve; E – Fresh porcine pulmonary valve; F – mechanical valve.
Tested valvular models – A – Decellularized porcine aortic valve; B – Decellularized porcine pulmonary valve; C- Biological prosthesis; D – Fresh porcine aortic valve; E – Fresh porcine pulmonary valve; F – mechanical valve.

Figure 2

Ultrasound and photographic examination – A – general aspects of the heart valve bioreactor for pulmonary conditions and transversal plane technique, the ultrasound probe is marked in green; B – ultrasound aspect of longitudinal plane examination – the leaflets are marked with the red arrow; C- ultrasound view of transversal plane examination – the central coaptation of cusps is marked with the blue arrow. D – Snapshot of a decellularized porcine aortic valve; E – Snapshot of a biological prosthesis; F – Picture editing of the mechanical prosthesis used to calculate the opening area; G – Software evaluation of the opening are of a biological prosthesis.
Ultrasound and photographic examination – A – general aspects of the heart valve bioreactor for pulmonary conditions and transversal plane technique, the ultrasound probe is marked in green; B – ultrasound aspect of longitudinal plane examination – the leaflets are marked with the red arrow; C- ultrasound view of transversal plane examination – the central coaptation of cusps is marked with the blue arrow. D – Snapshot of a decellularized porcine aortic valve; E – Snapshot of a biological prosthesis; F – Picture editing of the mechanical prosthesis used to calculate the opening area; G – Software evaluation of the opening are of a biological prosthesis.

Ultrasound examination results in pulmonary and aortic conditions

Pulmonary conditions
TEHV Scaffold Fresh Pulmonary valve Biological valve prosthesis Mechanical valve prosthesis
V max (cm/sec) 328 ± 85 355 ± 25 260 ± 41 181 ± 39
V mean (cm/sec) 127 ± 28 134 ± 10 124 ± 19 111 ± 7
P max (mmHg) 55.12 (19.00–60.78) 50.86 (45.98–57.63) 26.89 (18.09–38.25) 13.60 (7.36–20.40)
P mean (mmHg) 11.82 ± 5.42 12.26 ± 1.58 9.04 ± 2.47 4.42 ± 2.10
VTI (cm) 45.54 ± 9.83 47.46 ± 2.09 42.23 ± 7.23 30.71 ± 7.54
Functional area (cm2) 1.14 (1.07–1.92) 1.24 (1.17–1.29) 1.43 (1.24–1.69) 2.09 (1.68–3.03)
Aortic conditions
TEHV Scaffold Fresh Aortic valve Biological valve prosthesis Mechanical valve prosthesis
V max (cm/sec) 431 ± 64 469 ±143 368 ± 60 399 ±121
V mean (cm/sec) 130 ± 33 163 ± 43 132 ± 21 161 ± 8
P max (mmHg) 68.27 (60.28–100.75) 75.37 (46.78–158.50) 64.71 (33.00–68.08) 85.60 (23.83–101.50)
P mean (mmHg) 9.42 (8.08–22.00) 14.69 (9.60–30.67) 13.83 (7.00–14.51) 17.15 (7.10–18.00)
VTI (cm) 45.99 ± 11.32 51.13 ± 15.84 46.25 ± 7.64 48.08 ± 12.94
Functional area (cm2) 1.28 ± 0.27 1.14 ± 0.34 1.32 ± 0.26 1.26 ± 0.34

Video analysis in aortic and pulmonary conditions

TEHV scaffold Fresh valve Biological valve prosthesis Mechanical valve prosthesis
Normalized pulmonary conditions peak opening area (cm2) 2.916±0.102 3.459±0.099 2.018±0.013 2.920±0.106
Normalized aortic conditions peak opening area (cm2) 1.614±0.046 3.330±0.144 1.818±0.087 2.756±0.015
eISSN:
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Sprache:
Englisch
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4 Hefte pro Jahr
Fachgebiete der Zeitschrift:
Medizin, Klinische Medizin, Allgemeinmedizin, Innere Medizin, Kardiologie, Kinder- und Jugendmedizin, Kinderkardiologie, Chirurgie, Herzchirurgie
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