<abstract xmlns="http://www.w3.org/1999/xhtml"><p><italic>Salmonella enterica</italic> subsp. <italic>enterica</italic> serovar Typhimurium (ST) is an intracellularly parasitic bacterium. This zoonotic pathogen causes food poisoning and thus imposes a severe threat to food safety. Here, to understand the regulatory roles of the novel transcription factor STM0859 on the response of ST to environmental stress and biofilm formation, the <italic>STM0859</italic> gene-deficient strain and the complementation strain <italic>ΔSTM0859/STM0859</italic> were generated, respectively. Then, its capacity of responding to environmental stresses and biofilm (BF) formation ability under different stresses, including acid, alkali, high salt, cholate, and oxidative stresses was tested. We further analyzed the interaction between the STM0859 protein and the promoter of the acid stress response-related gene <italic>rcsB</italic> by performing an electrophoresis mobility shift assay (EMSA). The results showed that acid resistance and BF formation capacities of ST-<italic>ΔSTM0859</italic> strain were significantly weaker, as compared with those of <italic>Salmonella</italic> Typhimurium SL1344 (ST-SL1344) wild strain (<italic>p</italic> &lt; 0.01). Quantitative qRT-PCR analysis showed that the expression levels of acid stress and BF formation-related genes, <italic>rcsB</italic> and <italic>rpoS</italic>, of ST-<italic>ΔSTM0859</italic> strain were significantly reduced at the transcription levels, while the transcription levels of these genes were fully restored in complementation strain ST-<italic>ΔSTM0859</italic>/<italic>STM0859</italic>. The results of EMSA showed that STM0859 was capable of binding the promoter DNA fragments of the <italic>rcsB</italic> gene, suggesting that STM0859 can promote the transcription of the <italic>rcsB</italic> gene through interaction with its promoter, thereby exerting an indirectly regulatory role on the adaptive responses to acid stress and BF formation of ST. This study provided new insights into the regulatory mechanisms of the LysR family factors on the tolerances of ST under adverse environmental stresses.</p></abstract>

Salmonella enterica subsp. enterica serovar Typhimurium (ST) is an intracellularly parasitic bacterium. This zoonotic pathogen causes food poisoning and thus imposes a severe threat to food safety. Here, to understand the regulatory roles of the novel transcription factor STM0859 on the response of ST to environmental stress and biofilm formation, the STM0859 gene-deficient strain and the complementation strain ΔSTM0859/STM0859 were generated, respectively. Then, its capacity of responding to environmental stresses and biofilm (BF) formation ability under different stresses, including acid, alkali, high salt, cholate, and oxidative stresses was tested. We further analyzed the interaction between the STM0859 protein and the promoter of the acid stress response-related gene rcsB by performing an electrophoresis mobility shift assay (EMSA). The results showed that acid resistance and BF formation capacities of ST-ΔSTM0859 strain were significantly weaker, as compared with those of Salmonella Typhimurium SL1344 (ST-SL1344) wild strain (p < 0.01). Quantitative qRT-PCR analysis showed that the expression levels of acid stress and BF formation-related genes, rcsB and rpoS, of ST-ΔSTM0859 strain were significantly reduced at the transcription levels, while the transcription levels of these genes were fully restored in complementation strain ST-ΔSTM0859/STM0859. The results of EMSA showed that STM0859 was capable of binding the promoter DNA fragments of the rcsB gene, suggesting that STM0859 can promote the transcription of the rcsB gene through interaction with its promoter, thereby exerting an indirectly regulatory role on the adaptive responses to acid stress and BF formation of ST. This study provided new insights into the regulatory mechanisms of the LysR family factors on the tolerances of ST under adverse environmental stresses.

A Novel LysR Family Factor STM0859 is Associated with The Responses of <italic>Salmonella</italic> Typhimurium to Environmental Stress and Biofilm Formation

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

A Novel LysR Family Factor STM0859 is Associated with The Responses of  Typhimurium to Environmental Stress and Biofilm Formation

Salmonella enterica subsp. enterica serovar Typhimurium (ST) is an intracellularly parasitic bacterium. This zoonotic pathogen causes food poisoning and thus...

A Novel LysR Family Factor STM0859 is Associated with The Responses of Salmonella Typhimurium to Environmental Stress and Biofilm Formation

Article Category: original-paper

Online veröffentlicht: 20. Dez. 2021

Seitenbereich: 479 - 487

DOI: https://doi.org/10.33073/pjm-2021-045

Schlüsselwörter
Typhimurium, biofilm, environmental stress, gene

© 2021 Zhongmei Ma et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

A Novel LysR Family Factor STM0859 is Associated with The Responses of Salmonella Typhimurium to Environmental Stress and Biofilm Formation

Article Category: original-paper

Online veröffentlicht: 20. Dez. 2021

Seitenbereich: 479 - 487

DOI: https://doi.org/10.33073/pjm-2021-045

SchlüsselwörterTyphimurium, biofilm, environmental stress, gene

© 2021 Zhongmei Ma et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Schlüsselwörter
Typhimurium, biofilm, environmental stress, gene