<abstract xmlns="http://www.w3.org/1999/xhtml">Carbapenem-resistant <italic>Klebsiella pneumoniae</italic> (CRKP) is an important bacterium of nosocomial infections. In this study, CRKP strains, which were mainly isolated from fecal samples of 14 patients in three wards of the hospital in the Silesia Voivodship, rapidly increased from February to August 2018. Therefore, we conducted microbiological and molecular studies of the CRKP isolates analyzed. Colonized patients had critical underlying diseases and comorbidities; one developed bloodstream infection, and five died (33.3%). Antibiotic susceptibilities were determined by the E-test method. A disc synergy test confirmed carbapenemase production. CTX-Mplex PCR evaluated the presence of resistance genes <italic>bla</italic>CTX-M-type, <italic>bla</italic>CTX-M-1, <italic>bla</italic>CTX-M-9, and the genes <italic>bla</italic>SHV, <italic>bla</italic>TEM, <italic>bla</italic>KPC-2, <italic>bla</italic>NDM-1, <italic>bla</italic>OXA-48, <italic>bla</italic>IMP, and <italic>bla</italic>VIM-1 was detected with the PCR method. Clonality was evaluated by Multi Locus Sequence Typing (MLST) and Pulsed Field Gel Electrophoresis (PFGE). Six (40%) strains were of XDR (Extensively Drug-Resistant) phenotype, and nine (60%) of the isolates exhibited MDR (Multidrug-Resistant) phenotype. The range of carbapenem minimal inhibitory concentrations (MICs, μg/mL) was as follows doripenem (16 to >32), ertapenem (> 32), imipenem (4 to > 32), and meropenem (> 32). PCR and sequencing confirmed the <italic>bla</italic>CTX-M-15, <italic>bla</italic>KPC-2, <italic>bla</italic>OXA-48, and <italic>bla</italic>VIM-1 genes in all strains. The isolates formed one large PFGE cluster (clone A). MLST assigned them to the emerging high-risk clone of ST147 (CC147) pandemic lineage harboring the <italic>bla</italic>OXA-48 gene. This study showed that the <italic>K. pneumoniae</italic> isolates detected in the multi-profile medical centre in Katowice represented a single strain of the microorganism spreading in the hospital environment.</abstract>

Carbapenem-resistant Klebsiella pneumoniae (CRKP) is an important bacterium of nosocomial infections. In this study, CRKP strains, which were mainly isolated from fecal samples of 14 patients in three wards of the hospital in the Silesia Voivodship, rapidly increased from February to August 2018. Therefore, we conducted microbiological and molecular studies of the CRKP isolates analyzed. Colonized patients had critical underlying diseases and comorbidities; one developed bloodstream infection, and five died (33.3%). Antibiotic susceptibilities were determined by the E-test method. A disc synergy test confirmed carbapenemase production. CTX-Mplex PCR evaluated the presence of resistance genes blaCTX-M-type, blaCTX-M-1, blaCTX-M-9, and the genes blaSHV, blaTEM, blaKPC-2, blaNDM-1, blaOXA-48, blaIMP, and blaVIM-1 was detected with the PCR method. Clonality was evaluated by Multi Locus Sequence Typing (MLST) and Pulsed Field Gel Electrophoresis (PFGE). Six (40%) strains were of XDR (Extensively Drug-Resistant) phenotype, and nine (60%) of the isolates exhibited MDR (Multidrug-Resistant) phenotype. The range of carbapenem minimal inhibitory concentrations (MICs, μg/mL) was as follows doripenem (16 to >32), ertapenem (> 32), imipenem (4 to > 32), and meropenem (> 32). PCR and sequencing confirmed the blaCTX-M-15, blaKPC-2, blaOXA-48, and blaVIM-1 genes in all strains. The isolates formed one large PFGE cluster (clone A). MLST assigned them to the emerging high-risk clone of ST147 (CC147) pandemic lineage harboring the blaOXA-48 gene. This study showed that the K. pneumoniae isolates detected in the multi-profile medical centre in Katowice represented a single strain of the microorganism spreading in the hospital environment.

Clonal Dissemination of KPC-2, VIM-1, OXA-48-Producing <italic>Klebsiella pneumoniae</italic> ST147 in Katowice, Poland

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Clonal Dissemination of KPC-2, VIM-1, OXA-48-Producing  ST147 in Katowice, Poland

Carbapenem-resistant Klebsiella pneumoniae (CRKP) is an important bacterium of nosocomial infections. In this study, CRKP strains, which were mainly isolated...

Clonal Dissemination of KPC-2, VIM-1, OXA-48-Producing Klebsiella pneumoniae ST147 in Katowice, Poland

Article Category: original-paper

Online veröffentlicht: 19. März 2021

Seitenbereich: 107 - 116

Eingereicht: 27. Okt. 2020

Akzeptiert: 08. Feb. 2021

DOI: https://doi.org/10.33073/pjm-2021-010

Schlüsselwörter
clonal dissemination, carbapenem-resistant, hospital, PFGE, MLST

© 2021 Dorota Ochońska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Clonal Dissemination of KPC-2, VIM-1, OXA-48-Producing Klebsiella pneumoniae ST147 in Katowice, Poland

Article Category: original-paper

Online veröffentlicht: 19. März 2021

Seitenbereich: 107 - 116

Eingereicht: 27. Okt. 2020

Akzeptiert: 08. Feb. 2021

DOI: https://doi.org/10.33073/pjm-2021-010

Schlüsselwörterclonal dissemination, carbapenem-resistant, hospital, PFGE, MLST

© 2021 Dorota Ochońska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Schlüsselwörter
clonal dissemination, carbapenem-resistant, hospital, PFGE, MLST