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Biofilm-Forming Ability and Effect of Sanitation Agents on Biofilm-Control of Thermophile Geobacillus sp. D413 and Geobacillus toebii E134

TUGBA KİLİC

TUGBA KİLİC

  • Graduate School of Natural and Applied Sciences, Ankara UniversityAnkara, Turkey
  • Vocational School of Health Services, Medical Laboratory Techniques Program, Gazi UniversityAnkara, Turkey
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KİLİC, TUGBA
  
23. Nov. 2020
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Artikel-Kategorie: original-paper
Online veröffentlicht: 23. Nov. 2020
Seitenbereich: 411 - 419
Eingereicht: 11. Mai 2020
Akzeptiert: 02. Sept. 2020
DOI: https://doi.org/10.33073/pjm-2020-042
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© 2020 Tugba Kilic et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Agarose gel electrophoresis photographs displaying differences between the gDNA and eDNA of D413 and E134. M, Marker (Fermentas Gene Ruler 1 kb Plus DNA Ladder, 75–20.000 bp) (A) DNase I (1.45 mg/ml), RNase A (0.90 mg/ml) and proteinase K (0.85 mg/ml) treatment of both the gDNA and eDNA; (B) Different DNase I concentrations on eDNA (1.7, 2.5 and 3.0 mg/ml).
Agarose gel electrophoresis photographs displaying differences between the gDNA and eDNA of D413 and E134. M, Marker (Fermentas Gene Ruler 1 kb Plus DNA Ladder, 75–20.000 bp) (A) DNase I (1.45 mg/ml), RNase A (0.90 mg/ml) and proteinase K (0.85 mg/ml) treatment of both the gDNA and eDNA; (B) Different DNase I concentrations on eDNA (1.7, 2.5 and 3.0 mg/ml).

Fig. 2.

Treatment of mature biofilms with DNase I. P < 0.05* for comparisons of data obtained in the absence and in the presence of DNase I (Dunnett’s test).
Treatment of mature biofilms with DNase I. P < 0.05* for comparisons of data obtained in the absence and in the presence of DNase I (Dunnett’s test).

Fig. 3.

The viable cell counts of D413 biofilms formed on surfaces (Tukey test; p < 0.05*).
The viable cell counts of D413 biofilms formed on surfaces (Tukey test; p < 0.05*).

Fig. 4.

The viable cell counts of E134 biofilms formed on surfaces (Tukey test; p < 0.05*).
The viable cell counts of E134 biofilms formed on surfaces (Tukey test; p < 0.05*).

Fig. 5.

The effects of sanitation agents on the biofilm of D413 and E134 isolates (Dunnett’s test; p < 0.05*).
The effects of sanitation agents on the biofilm of D413 and E134 isolates (Dunnett’s test; p < 0.05*).

Treatment of biofilms with sanitation agents_

Agent (Concentration)Temperature – TimeReferences
AP (0.16 U/g)      37°C - 60 minParkar et al. 2004
Protease 0.16 U/g)      37°C - 60 minParkar et al. 2004
Subtilisin (1%)      37°C - 30 minParkar et al. 2004
Trypsin (3%)      37°C - 3 hParkar et al. 2003
SDS (3%)    100°C - 10 minParkar et al. 2003
a-Amylase (1%)      37°C - 30 minParkar et al. 2004
Cellulase (1.66%)      37°C - 30 minParkar et al. 2004
SM (100 mM)      22°C - 60 minParkar et al. 2003
Lysozyme (2%)      37°C - 60 minParkar et al. 2003
TCA (10%)    100°C - 15 minParkar et al. 2003
Nisin (2 mg/ml)      37°C - 24 hParkar et al. 2003
PM (2 mg/ml)      22°C - 30 minParkar et al. 2003
ST (10 mg/ml)      22°C - 5 minParkar et al. 2003
2(5H)-Furanone (1 mg/ml)      22°C - 60 minPonnusamy et al. 2010
Triclosan (2 mg/ml)      22°C - 60 minTabak et al. 2007
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