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Anti-Salmonella Potential of New Lactobacillus Strains with the Application in the Poultry Industry

JUSTYNA D. KOWALSKA
JUSTYNA D. KOWALSKA
, 
ADRIANA NOWAK
ADRIANA NOWAK
, 
KATARZYNA ŚLIŻEWSKA
KATARZYNA ŚLIŻEWSKA
, 
MAŁGORZATA STAŃCZYK
MAŁGORZATA STAŃCZYK
, 
MAGDALENA ŁUKASIAK
MAGDALENA ŁUKASIAK
 und 
JAROSŁAW DASTYCH
JAROSŁAW DASTYCH
   | 28. Jan. 2020
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Article Category: original-paper
Online veröffentlicht: 28. Jan. 2020
Seitenbereich: 5 - 18
Eingereicht: 03. Okt. 2019
Akzeptiert: 02. Dez. 2019
DOI: https://doi.org/10.33073/pjm-2020-001
Schlüsselwörter
© 2020 Justyna D. Kowalska et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

The survival of lactic acid bacteria at 1% and 2% bile salts.
The survival of lactic acid bacteria at 1% and 2% bile salts.

Fig. 2.

The resistance of LAB to low pH.
The resistance of LAB to low pH.

Fig. 3.

Adherence of Salmonella sp. strains to adenocarcinoma cells Caco-2 in the presence of lactic acid bacteria presented as stimulation/inhibition rate (%).
Adherence of Salmonella sp. strains to adenocarcinoma cells Caco-2 in the presence of lactic acid bacteria presented as stimulation/inhibition rate (%).

Fig. 4.

Cytotoxic effect of probiotics’ supernatants on Caco-2 cells in the range of tested dilutions presented as % of control.
Cytotoxic effect of probiotics’ supernatants on Caco-2 cells in the range of tested dilutions presented as % of control.

Fig. 5.

Total antagonistic activity index (IAA) of lactic acid bacteria.
Total antagonistic activity index (IAA) of lactic acid bacteria.

Antagonistic activity of Lactobacillus sp. strains against Salmonella sp. (type of inhibition: +++ strong; ++ moderate; + weak; − no inhibition).

Salmonella sp. strain Year of Salmonella acquisition L. rhamnosus LOCK 1131 L. casei LOCK 1132 L. paracasei LOCK 1133
S. Typhimurium ATCC 13311 +++ +++ +++
S. Typhimurium ATCC 14028 +++ +++ +++
S. Enteritidis ATCC 13076 +++ +++ +++
S. Enteritidis 1067/S/09 K-2 2009 + a + a +++ b
S. Enteritidis 848/S/09 2009 ++ a + b ++ a
S. Enteritidis Ferma ‘K’ K-9 2010 + a + a ++ b
S. Enteritidis Woź K-7 2010 + a ++ a ++ b
S. Enteritidis Ferma ‘K’ K-10 2010 ++ a + b ++ a
S. Enteritidis Ferma ‘K’ K-11 2010 ++ a + b ++ a
S. Enteritidis Ferma ‘K’ K-12 2010 + a + b + a
S. Enteritidis Ferma ‘K’ K-3 2010 + a ++ b + a
S. Enteritidis Ferma ‘K’ K-4 2010 + a ++ b + a
S. Enteritidis Ferma ‘K’ K-6 2010 + a + b + a
S. Enteritidis Ferma ‘K’ K-14 2010 + b + a ++ a
S. Enteritidis Ferma ‘K’ K-18 2010 ++ a + ab + b
S. Enteritidis Pac K-piętro 2010 + ab + a + b
S. Enteritidis Woź K-6 2010 + + +
S. Enteritidis Woź K-9 2010 ++ a + ab + b
S. Enteritidis 64/S/10 2010 + + +
S. Enteritidis 249 2010 + + +
S. Enteritidis 517/S/09 2009 ++ a + ab + b
S. Enteritidis 571/S/09 2009 + a + b ++ c
S. Enteritidis 833/S/09 2009 + + ++
S. Enteritidis 838/S/09 2009 ++ b + a + a
S. Enteritidis 838/S/09 B 2009 + + ++
S. Enteritidis 847/S/09 2009 + a + a − b
S. Enteritidis 865/S/09 2009 + + +
S. Enteritidis 866/S/09 2009 + a − b + a
S. Enteritidis 945/S/09 2009 ++ b + a − a
S. Enteritidis 975/S/09 2009 + + +
S. Enteritidis 1013/S/09 K-4 2009 + a ++ b + a
S. Enteritidis 1014/S/09 K-1 2009 + b ++ a + a
S. Enteritidis 1021/S/09 2009 ++ b + a + a
S. Enteritidis 1022/S/09 K-9 2009 ++ a ++ a + b
S. Enteritidis 1542/S/09 NWJ 2009 + ab + a + b
S. Enteritidis 1044/S/09 K-2 2009 + a − b + a
S. Enteritidis 1445/S/09 K-46 2009 + + +
S. Enteritidis 1446/S/09 K-31 2009 ++ b + a + a
S. Enteritidis 1465/S/09 MEK 2009 + a − b + a
S. Enteritidis 1515/S/09 2009 + a + ab + b
S. Enteritidis 1535/S/09 NWJ 2009 + b + a + a
S. Enteritidis 1714/09 2009 + + +
S. Enteritidis 2050/S/09 2009 + a + ab + b
S. Enteritidis 1085/S/09 MEK 2009 ++ a ++ b + c
S. Enteritidis 1231/S/09 2009 + + +
S. Enteritidis 1206/S/09 MEK 2009 ++ a + b − c
S. Enteritidis 1171/S/09 2009 a ++ b + c
S. Enteritidis 1257/S/09 K7 2009 ++ a ++ b + c
S. Enteritidis 1250/S/09 2009 + a ++ b + a
S. Enteritidis 1143/S/09 2009 + a ++ b ++ ab
S. Enteritidis 1422/S/09 MEK 2009 + a ++ b + c
S. Enteritidis 1106/S/09 K-1 2009 + a + a ++ b
S. Enteritidis 1061/S/09/K-5 2009 ++ b ++ a ++ a
S. Enteritidis 1067/S/09 K-3 2009 + ++ +
S. Enteritidis 1573/S/09 NWJ 2009 ++ a ++ b ++ c
S. Enteritidis 1192/S/09 K-8 2009 ++ ab ++ a + b
S. Enteritidis 1047/S/09 K-1 2009 ++ a ++ a + b
S. Enteritidis 1545/S/09 MEK 2009 + ab ++ a + b
S. Enteritidis 1048/S/09 K-8 2009 + + +
S. Enteritidis 1572/S/09 NWJ 2009 ++ ab ++ a ++ b
S. Enteritidis 002PP2014 2014 + a ++ ab ++ b
S. Enteritidis 004PP2014 2014 + a ++ b ++ b
S. Enteritidis 005PP2014 2014 ++ a + b + c
S. Enteritidis 001PP2014 2014 ++ ++ ++
S. Enteritidis 006PP2014 2014 + + +
S. Enteritidis 007PP2014 2014 ++ a + b ++ a
S. Enteritidis 2149/09 2009 ++ a + b ++ a
S. Enteritidis 2619/S/10 2010 + b + a + a
S. Enteritidis 003PP2014 2014 ++ a + a ++ b
S. Enteritidis 008PP2014 2014 + b ++ a ++ a
S. Enteritidis 009PP2014 2014 + a + b + a
S. Enteritidis 010PP2014 2014 + a + b + a
S. Enteritidis 12617/2/S 2014 + a + a ++ b
S. Enteritidis 12633 2014 + + +
S. Enteritidis 12961/1 2014 + b ++ a ++ a
S. Enteritidis 13001 2014 + a ++ ab ++ b
S. Enteritidis 011PP2014 2014 ++ a ++ b ++ ab
S. Enteritidis 012PP2014 2014 + a + a ++ b
S. Enteritidis 014PP2014 2014 + a + a ++ b
S. Enteritidis 015PP2014 2014 + a + a ++ b
S. Enteritidis 016PP2015 2015 + a + a ++ b
S. Enteritidis 017PP2015 2015 ++ a + b + ab
S. Enteritidis 018PP2015 2015 ++ ++ ++
S. Enteritidis 019PP2015 2015 ++ a + b + c
S. Enteritidis 020PP2016 2016 + + +
S. Enteritidis 021PP2016 2016 ++ ++ ++
S. Enteritidis 022PP2016 2016 + a + b ++ a
S. Enteritidis 023PP2016 2016 + ++ +
S. Enteritidis 024PP2016 2016 + a + b ++ c
S. Enteritidis 025PP2016 2016 + + +
S. Enteritidis 026PP2016 2016 ++ a ++ ab + b
S. Enteritidis 027PP2016 2016 ++ a + b + c
S. Enteritidis 028PP2016 2016 + a − b + c
S. Enteritidis 029PP2016 2016 + a + b + a
S. Enteritidis 030PP2016 2016 + a - b + a
S. Enteritidis 031PP2016 2016 + + +
S. Enteritidis 032PP2016 2016 ++ a ++ b ++ c
S. Enteritidis 033PP2016 2016 ++ ++ ++
S. Enteritidis 034PP2016 2016 + + +
S. Enteritidis 035PP2016 2016 ++ a ++ b + c
S. Enteritidis 036PP2016 2016 ++ ++ ++
S. Enteritidis 037PP2016 2016 + a ++ b + a
S. Enteritidis 038PP2016 2016 ++ a + b ++ a
S. Enteritidis 039PP2016 2016 ++ ++ +
S. Enteritidis 040PP2016 2016 ++ a ++ b ++ ab
S. Enteritidis 041PP2016 2016 ++ b ++ a ++ a
S. Enteritidis 042PP2016 2016 ++ ++ ++
S. Enteritidis 043PP2016 2016 + a + a ++ b
S. Enteritidis 044PP2016 2016 ++ a +++ b ++ a
S. Enteritidis 045PP2016 2016 ++ +++ ++
S. Enteritidis 046PP2016 2016 + ++ +
S. Enteritidis 047PP2016 2016 ++ a ++ b ++ ab
S. Enteritidis 048PP2016 2016 + b + a + a
S. Enteritidis 049PP2016 2016 ++ a +++ b + c
S. Enteritidis 050PP2016 2016 ++ a + a + b
S. Enteritidis 051PP2016 2016 +++ +++ +++
S. Enteritidis 53A 2013 ++ a + b ++ a
S. Enteritidis Korczak 28A 2013 ++ a + b ++ a
S. Enteritidis Korczak 32A 2013 + + +
S. Enteritidis Korczak 40A 2013 ++ a + b + c
S. Enteritidis Korczak 41A 2013 ++ a + b ++ a
S. Enteritidis 4050/2014 D09 2014 ++ a ++ b ++ ab
S. Enteritidis 6537/1/S/14 D09 2014 + a + b + ab
S. Enteritidis 6670/1/S/14 D09 2014 + a + a + b
S. Enteritidis 7660/1/D03 2014 + a + b + c
S. Enteritidis 65/S/10 2010 + a + ab + b
S. Enteritidis 1KSFA 2013 ++ a + ab + b

Probiotic properties of Lactobacillus strains.

Autoaggregation Coaggregation Coefficient of adherence to polystyrene Adherence to Caco-2 cells
S. Ent. 13076 S. Typ. 13311 S. Typ. 14028 Init. No. After 2 h Adherence index
[log CFU/ml]
L. rhamnosus LOCK 1131 96.1% ± 1.2% 14.8% ± 0.01% 84.2% ± 0.01% 51.8% ± 0.03% 1.18 ± 0.02 8.5 7.6 ± 1.1 89.8%
L. casei LOCK 1132 98.2% ± 3.6% 26.1% ± 0.01% 58.5% ± 0.01% −11.1%± 0.01% 1.22 ± 0.02 8.5 8.3 ± 0.5 98.0%
L. paracasei LOCK 1133 93.5% ± 0.9% 41.8% ± 3.4% 17.8% ± 3.3% 43.7% ± 2.7% 1.20 ± 0.07 8.0 6.3 ± 0.1 78.9%

Antibiotic resistance of Lactobacillus strains.

Antibiotic inhibition zone diameter[mm ± SD]
PEN KAN TET ERY DOX AMX
L. rhamnosus LOCK 1131 27 ± 2.5(S) 0(R) 34 ± 1.0(S) 33 ± 2.5(S) 32 ± 2.0(S) 21 ± 2.1(R)
L. casei LOCK 1132 28 ± 2.5(S) 0(R) 30 ± 0.6(S) 34 ± 1.5(S) 33 ± 1.0(S) 24 ± 0.6(IR)
L. paracasei LOCK 1133 27 ± 6.4(S) 0(R) 25 ± 1.5(S) 25 ± 0.0(S) 30 ± 0.6(S) 21 ± 1.0(R)
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