<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Various microbial biomasses have been employed as biosorbents. Bacterial biomass has added advantages because of easy in production at a low cost. The study investigated the biosorption of iron from aqueous solutions by <italic>Bacillus subtilis</italic>. An optimum biosorption capacity of 7.25 mg of the metal per gram of the biosorbent was obtained by the Inductive Coupled Plasma Optical Emission Spectroscopy (ICP-OES) under the experimental conditions of initial metal concentration of 100 mg/l, pH 4.5, and biomass dose of 1 g/l at 30°C for 24 hrs. The data showed the best fit with the Freundlich isotherm model while following pseudo-first-order kinetics. Scanning Electron Microscope (SEM) and Energy Dispersive X-ray (EDX) analysis confirmed iron biosorption as precipitates on the bacterial surface, and as a peak in the EDX spectrum. The functional hydroxyl, carboxyl, and amino groups that are involved in biosorption were revealed by the Fourier Transform Infrared spectroscopy (FTIR). The amorphous nature of the biosorbent for biosorption was indicated by the X-ray Diffraction (XRD) analysis. The biomass of <italic>B. subtilis</italic> exhibited a point zero charge (pH<sub>pzc</sub>) at 2.0.</p></abstract>

Various microbial biomasses have been employed as biosorbents. Bacterial biomass has added advantages because of easy in production at a low cost. The study investigated the biosorption of iron from aqueous solutions by Bacillus subtilis. An optimum biosorption capacity of 7.25 mg of the metal per gram of the biosorbent was obtained by the Inductive Coupled Plasma Optical Emission Spectroscopy (ICP-OES) under the experimental conditions of initial metal concentration of 100 mg/l, pH 4.5, and biomass dose of 1 g/l at 30°C for 24 hrs. The data showed the best fit with the Freundlich isotherm model while following pseudo-first-order kinetics. Scanning Electron Microscope (SEM) and Energy Dispersive X-ray (EDX) analysis confirmed iron biosorption as precipitates on the bacterial surface, and as a peak in the EDX spectrum. The functional hydroxyl, carboxyl, and amino groups that are involved in biosorption were revealed by the Fourier Transform Infrared spectroscopy (FTIR). The amorphous nature of the biosorbent for biosorption was indicated by the X-ray Diffraction (XRD) analysis. The biomass of B. subtilis exhibited a point zero charge (pHpzc) at 2.0.

Structural Changes of <italic>Bacillus subtilis</italic> Biomass on Biosorption of Iron (II) from Aqueous Solutions: Isotherm and Kinetic Studies

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Structural Changes of  Biomass on Biosorption of Iron (II) from Aqueous Solutions: Isotherm and Kinetic Studies

Various microbial biomasses have been employed as biosorbents. Bacterial biomass has added advantages because of easy in production at a low cost. The study...

Langmuir isotherm		Freundlich isotherm		Temkin isotherm
q_max (mg/g)	3.831	n	3.425	B (J/mol)	1.3583
K_L (l/mg)	1.845	K_F (mg/g)	2.766	A (l/g)	6.78 g/l
R²	0.9353	R²	0.9057	R²	0.8715
R_L	0.013

Pseudo-first-order kinetic model			Pseudo-second-order kinetic model
q_e (mg/g),	K₁ (min^–1)	R²	q_e (mg/g)	K₂ (gmg^–1 min^–1)	R²
10.6	0.2001	0.9201	17.76	0.0042	0.7154

Structural Changes of Bacillus subtilis Biomass on Biosorption of Iron (II) from Aqueous Solutions: Isotherm and Kinetic Studies

Article Category: original-paper

Online veröffentlicht: 05. Dez. 2019

Seitenbereich: 549 - 558

Eingereicht: 09. Juni 2019

Akzeptiert: 11. Nov. 2019

DOI: https://doi.org/10.33073/pjm-2019-057

Schlüsselwörter
biosorption, iron, isotherms, kinetics

© 2019 Sri Lakshmi Ramya Krishna Kanamarlapudi and Sudhamani Muddada et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Fig. 2.

Fig. 3.

Fig. 4.

Fig. 5.

Fig. 6a.

Fig. 6b.

Fig. 7a.

Fig. 7b.

Fig. 8.

Constants of Langmuir, Freundlich and Temkin isotherm models used for the biosorption of Fe (II) ions onto B. subtilis biomass.

Constants of pseudo-first and pseudo-second-order kinetic models obtained for the biosorption of Fe (II) ions onto B. subtilis.

Structural Changes of Bacillus subtilis Biomass on Biosorption of Iron (II) from Aqueous Solutions: Isotherm and Kinetic Studies

Article Category: original-paper

Online veröffentlicht: 05. Dez. 2019

Seitenbereich: 549 - 558

Eingereicht: 09. Juni 2019

Akzeptiert: 11. Nov. 2019

DOI: https://doi.org/10.33073/pjm-2019-057

Schlüsselwörterbiosorption, iron, isotherms, kinetics

© 2019 Sri Lakshmi Ramya Krishna Kanamarlapudi and Sudhamani Muddada et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Fig. 2.

Fig. 3.

Fig. 4.

Fig. 5.

Fig. 6a.

Fig. 6b.

Fig. 7a.

Fig. 7b.

Fig. 8.

Constants of Langmuir, Freundlich and Temkin isotherm models used for the biosorption of Fe (II) ions onto B. subtilis biomass.

Constants of pseudo-first and pseudo-second-order kinetic models obtained for the biosorption of Fe (II) ions onto B. subtilis.

Schlüsselwörter
biosorption, iron, isotherms, kinetics