<abstract xmlns="http://www.w3.org/1999/xhtml">

 The aim of the study was to investigate the effect of 2-methoxyestradiol (2-ME) on GnRH-induced LH and FSH secretion by porcine pituitary cells <italic>in vitro</italic>. Moreover, the concentrations of superoxide anion radical (O2-), as a possible mediator of 2-ME action, and malonyldialdehyde (MDA), as an oxidative stress indicator, were estimated. Pituitary cells were cultured in McCoy 5A medium with GnRH (positive control), with GnRH and 3.3×10-11-3.3×10-7M/L of 2-ME, or with GnRH and 2.7×10-9- 2.7×10-8 M/L of 17β-estradiol. The secretion of gonadotropins, as well as concentrations of superoxide anion and MDA were analysed after 2-72 h of the experiment. A positive correlation between 2-ME dose and FSH secretion from anterior pituitary cells (r=0.72, 0.95, 0.92, 0.90, 0.85, and 0.82 after 2, 6, 18, 24, 48, and 72 h, respectively) was demonstrated. In contrast, a negative correlation between 2-ME concentration and LH secretion was observed (r=-0.82, -0.72, -0.85, -0.89, -0.93, and -0.79 after 2, 6, 18, 24, 48, and 72 h, respectively). The concentration of O2-. was increasing under the influence of the rising doses of 2-ME, whereas the mean level of MDA was not changing significantly.
</abstract>

The aim of the study was to investigate the effect of 2-methoxyestradiol (2-ME) on GnRH-induced LH and FSH secretion by porcine pituitary cells in vitro. Moreover, the concentrations of superoxide anion radical (O2-), as a possible mediator of 2-ME action, and malonyldialdehyde (MDA), as an oxidative stress indicator, were estimated. Pituitary cells were cultured in McCoy 5A medium with GnRH (positive control), with GnRH and 3.3×10-11-3.3×10-7M/L of 2-ME, or with GnRH and 2.7×10-9- 2.7×10-8 M/L of 17β-estradiol. The secretion of gonadotropins, as well as concentrations of superoxide anion and MDA were analysed after 2-72 h of the experiment. A positive correlation between 2-ME dose and FSH secretion from anterior pituitary cells (r=0.72, 0.95, 0.92, 0.90, 0.85, and 0.82 after 2, 6, 18, 24, 48, and 72 h, respectively) was demonstrated. In contrast, a negative correlation between 2-ME concentration and LH secretion was observed (r=-0.82, -0.72, -0.85, -0.89, -0.93, and -0.79 after 2, 6, 18, 24, 48, and 72 h, respectively). The concentration of O2-. was increasing under the influence of the rising doses of 2-ME, whereas the mean level of MDA was not changing significantly.

Effect of 2-Methoxyestradiol on Gonadotropin Secretion and Oxidative Status of Porcine Pituitary Cells In Vitro

Department of Pathophysiology, Chair of Preclinical Veterinary Sciences, Faculty of Veterinary Medicine

Journal of Veterinary Research

The aim of the study was to investigate the effect of 2-methoxyestradiol (2-ME) on GnRH-induced LH and FSH secretion by porcine pituitary cells in vitro....

Effect of 2-Methoxyestradiol on Gonadotropin Secretion and Oxidative Status of Porcine Pituitary Cells In Vitro

Online veröffentlicht: 17. Jan. 2013

Seitenbereich: 373 - 378

DOI: https://doi.org/10.2478/v10213-012-0065-6

Schlüsselwörter
swine, 2-methoxyestradiol, pituitary cells, FSH, LH

This content is open access.

Effect of 2-Methoxyestradiol on Gonadotropin Secretion and Oxidative Status of Porcine Pituitary Cells In Vitro

Online veröffentlicht: 17. Jan. 2013

Seitenbereich: 373 - 378

DOI: https://doi.org/10.2478/v10213-012-0065-6

Schlüsselwörterswine, 2-methoxyestradiol, pituitary cells, FSH, LH

This content is open access.

Schlüsselwörter
swine, 2-methoxyestradiol, pituitary cells, FSH, LH