<abstract xmlns="http://www.w3.org/1999/xhtml"> We evaluated the toxicity of hepatic, hematological, and oxidative effects of glyphosate-Roundup® on male and female albino Swiss mice. The animals were treated orally with either 50 or 500 mg/kg body weight of the herbicide, on a daily basis for a period of 15 days. Distilled water was used as control treatment. Samples of blood and hepatic tissue were collected at the end of the treatment. Hepatotoxicity was monitored by quantitative analysis of the serum enzymes ALT, AST, and γ-GT and renal toxicity by urea and creatinine. We also investigated liver tissues histopathologically. Alterations of hematological parameters were monitored by RBC, WBC, hemoglobin, hematocrit, MCV, MCH, and MCHC. TBARS (thiobarbituric acid reactive substances) and NPSH (non-protein thiols) were analyzed in the liver to assess oxidative damage. Significant increases in the levels of hepatic enzymes (ALT, AST, and γ-GT) were observed for both herbicide treatments, but no considerable differences were found by histological analysis. The hematological parameters showed significant alterations (500 mg/kg body weight) with reductions of RBC, hematocrit, and hemoglobin, together with a significant increase of MCV, in both sexes of mice. In males, there was an important increase in lipid peroxidation at both dosage levels, together with an NPSH decrease in the hepatic tissue, whereas in females significant changes in these parameters were observed only at the higher dose rate. The results of this study indicate that glyphosate-Roundup® can promote hematological and hepatic alterations, even at subacute exposure, which could be related to the induction of reactive oxygen species.</abstract>

We evaluated the toxicity of hepatic, hematological, and oxidative effects of glyphosate-Roundup® on male and female albino Swiss mice. The animals were treated orally with either 50 or 500 mg/kg body weight of the herbicide, on a daily basis for a period of 15 days. Distilled water was used as control treatment. Samples of blood and hepatic tissue were collected at the end of the treatment. Hepatotoxicity was monitored by quantitative analysis of the serum enzymes ALT, AST, and γ-GT and renal toxicity by urea and creatinine. We also investigated liver tissues histopathologically. Alterations of hematological parameters were monitored by RBC, WBC, hemoglobin, hematocrit, MCV, MCH, and MCHC. TBARS (thiobarbituric acid reactive substances) and NPSH (non-protein thiols) were analyzed in the liver to assess oxidative damage. Significant increases in the levels of hepatic enzymes (ALT, AST, and γ-GT) were observed for both herbicide treatments, but no considerable differences were found by histological analysis. The hematological parameters showed significant alterations (500 mg/kg body weight) with reductions of RBC, hematocrit, and hemoglobin, together with a significant increase of MCV, in both sexes of mice. In males, there was an important increase in lipid peroxidation at both dosage levels, together with an NPSH decrease in the hepatic tissue, whereas in females significant changes in these parameters were observed only at the higher dose rate. The results of this study indicate that glyphosate-Roundup® can promote hematological and hepatic alterations, even at subacute exposure, which could be related to the induction of reactive oxygen species.

Evaluation of biochemical, hematological and oxidative parameters in mice exposed to the herbicide glyphosate-Roundup®

Curso de Farmácia, Universidade Alto Vale do Rio do Peixe, Rua Victor Baptista Adami 800, Centro, Caçador, SC, Brazil

Universidade Federal de Pernambuco, Programa de Pós-Graduação em Biotecnologia Industrial, Departamento de Antibióticos, Av. Professor Moraes Rego, Cidade Universitária, Recife, PE, Brazil

Departamento de Antibióticos, Av. Professor Moraes Rego, Cidade Universitária, Recife, PE, Brazil 3 Departamento de Medicina Veterinária, Centro Agroveterinário, Universidade do Estado de Santa Catarina, Lages, SC, Brazil

Curso de Farmácia, Universidade Alto Vale do Rio do Peixe, Rua Victor Baptista Adami 800, Centro, Caçador, SC, 89500-000 Brazil. TEL.: +55 4935675541

Interdisciplinary Toxicology

We evaluated the toxicity of hepatic, hematological, and oxidative effects of glyphosate-Roundup® on male and female albino Swiss mice. The animals were...

Evaluation of biochemical, hematological and oxidative parameters in mice exposed to the herbicide glyphosate-Roundup®

Online veröffentlicht: 16. Jan. 2013

Seitenbereich: 133 - 140

DOI: https://doi.org/10.2478/v10102-012-0022-5

Schlüsselwörter
Glyphosate, Roundup®, hepatotoxicity, hematological damage, oxidative stress

This content is open access.

Evaluation of biochemical, hematological and oxidative parameters in mice exposed to the herbicide glyphosate-Roundup®

Online veröffentlicht: 16. Jan. 2013

Seitenbereich: 133 - 140

DOI: https://doi.org/10.2478/v10102-012-0022-5

SchlüsselwörterGlyphosate, Roundup®, hepatotoxicity, hematological damage, oxidative stress

This content is open access.

Schlüsselwörter
Glyphosate, Roundup®, hepatotoxicity, hematological damage, oxidative stress