<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml">A multi-photon polymerization system has been designed based on a pulsed irradiation light source (diode-pumped solid state femtosecond laser Yb:KGW, 300 fs, 1030 nm, 1-200 kHz) in combination with large working area and high precision linear motor driven stages (100×100×50 mm3). The system is intended for high resolution and throughput 3D micro- and nanofabrication and enables manufacturing the polymeric templates up to 1 cm2 areas with sub-micrometer resolution. These can be used for producing 3D artificial polymeric scaffolds to be applied for growing cells, specifically, in the tissue engineering. The bio-compatibility of different acrylate, hybrid organic-inorganic and biodegradable polymeric materials is evaluated experimentally <italic>in vitro</italic>. Variously sized and shaped polymeric scaffolds of biocompatible photopolymers with intricate 3D geometry were successfully fabricated. Proliferation tests for adult rabbit myogenic stem cells have shown the applicability of artificial scaffolds in biomedicine practice.</abstract>

A multi-photon polymerization system has been designed based on a pulsed irradiation light source (diode-pumped solid state femtosecond laser Yb:KGW, 300 fs, 1030 nm, 1-200 kHz) in combination with large working area and high precision linear motor driven stages (100×100×50 mm3). The system is intended for high resolution and throughput 3D micro- and nanofabrication and enables manufacturing the polymeric templates up to 1 cm2 areas with sub-micrometer resolution. These can be used for producing 3D artificial polymeric scaffolds to be applied for growing cells, specifically, in the tissue engineering. The bio-compatibility of different acrylate, hybrid organic-inorganic and biodegradable polymeric materials is evaluated experimentally in vitro. Variously sized and shaped polymeric scaffolds of biocompatible photopolymers with intricate 3D geometry were successfully fabricated. Proliferation tests for adult rabbit myogenic stem cells have shown the applicability of artificial scaffolds in biomedicine practice.

Laser-Micro/Nanofabricated 3D Polymers for Tissue Engineering Applications

Department of Quantum Electronics, Physics Faculty, Vilnius University, 9-3 Saulėtekio Av., Vilnius, LT-10223, LITHUANIA

Trakai Hospital, Faculty of Medicine, Vilnius University, 21 M.K.Čiurlionio Str., Vilnius, LT-03101, LITHUANIA

Vivarium, Institute of Biochemistry, Vilnius University, 12 Mokslininkų Str., Vilnius, LT-08662, LITHUANIA

Latvian Journal of Physics and Technical Sciences

{"article-title":"Laser-Micro/Nanofabricated 3D Polymers for Tissue Engineering Applications"}

A multi-photon polymerization system has been designed based on a pulsed irradiation light source (diode-pumped solid state femtosecond laser Yb:KGW, 300 fs,...

Laser-Micro/Nanofabricated 3D Polymers for Tissue Engineering Applications

Online veröffentlicht: 19. Mai 2011

Seitenbereich: 32 - 43

DOI: https://doi.org/10.2478/v10047-011-0013-x

Schlüsselwörter
femtosecond laser, micro- and nanofabrication, photopolymerization, expansion experiments, 3D nanolithography, artificial scaffolds, stem cells, regenerative medicine

This content is open access.

Laser-Micro/Nanofabricated 3D Polymers for Tissue Engineering Applications

Online veröffentlicht: 19. Mai 2011

Seitenbereich: 32 - 43

DOI: https://doi.org/10.2478/v10047-011-0013-x

Schlüsselwörterfemtosecond laser, micro- and nanofabrication, photopolymerization, expansion experiments, 3D nanolithography, artificial scaffolds, stem cells, regenerative medicine

This content is open access.

Schlüsselwörter
femtosecond laser, micro- and nanofabrication, photopolymerization, expansion experiments, 3D nanolithography, artificial scaffolds, stem cells, regenerative medicine