Males of Aphelenchoides limberi are described and illustrated for the first time from mushroom plate cultures of Botrytis cinerea. The original parthenogenetic population was extracted from the soil of a hop garden in Senice na Hané, Czech Republic. The males are characterized by a stylet about 11 μm long, a prominent spicula, with the dorsal limb longer than the ventral limb, and a ventrally curved tip. The tail is arcuate, conoid, ending with a short, mostly ventrally bent hook-shaped mucro, the lateral field appears to have four lines under light microscopy.