<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Purpose</bold>: Ovarian torsion, being a gynecological emergency, requires to be rapidly diagnosed and treated with minimal consequences on ovarian function after the removal of torsion. As ischemia modified albumin (IMA) is considered a good biomarker in diverse ischemic diseases, the aim of our study was to determine the effect of different ovarian torsion/detorsion models on serum and ovarian homogenates levels of IMA in an experimental study.<bold>Methods</bold>: IMA was measured in the serum and ovarian homogenates of 7 groups of female rats (10 animals in each group): 1 - control (no intervention); 2 - sham (only laparotomy); 3 - ischemia group: 3 hours ovarian torsion (OT); 4 - 3 hours OT (ischemia), 1 hour simple reperfusion; 5 - 3 hours ischemia, 1 hour controlled reperfusion that was assured during the first two minutes by opening and closing the clips on the ovarian pedicles in 10 seconds intervals, followed by simple reperfusion; 6 - 3 hours ischemia, 24 hours simple reperfusion; 7 - 3 hours OT, 24 hours controlled reperfusion. The results were analyzed by Welch’s ANOVA and Spearman correlation.<bold>Results</bold>: Ischemia increases the IMA in both serum and ovarian homogenates compared to control and sham groups. The controlled reperfusion groups had a statistically significant lower IMA in serum compared to simple reperfusion groups. IMA was found to be higher in the ovarian homogenates of simple reperfusion compared to controlled reperfusion groups.<bold>Conclusion</bold>: Our results suggest that controlled reperfusion prevent the processes that increase the IMA in ovarian torsion.</abstract>

Purpose: Ovarian torsion, being a gynecological emergency, requires to be rapidly diagnosed and treated with minimal consequences on ovarian function after the removal of torsion. As ischemia modified albumin (IMA) is considered a good biomarker in diverse ischemic diseases, the aim of our study was to determine the effect of different ovarian torsion/detorsion models on serum and ovarian homogenates levels of IMA in an experimental study.Methods: IMA was measured in the serum and ovarian homogenates of 7 groups of female rats (10 animals in each group): 1 - control (no intervention); 2 - sham (only laparotomy); 3 - ischemia group: 3 hours ovarian torsion (OT); 4 - 3 hours OT (ischemia), 1 hour simple reperfusion; 5 - 3 hours ischemia, 1 hour controlled reperfusion that was assured during the first two minutes by opening and closing the clips on the ovarian pedicles in 10 seconds intervals, followed by simple reperfusion; 6 - 3 hours ischemia, 24 hours simple reperfusion; 7 - 3 hours OT, 24 hours controlled reperfusion. The results were analyzed by Welch’s ANOVA and Spearman correlation.Results: Ischemia increases the IMA in both serum and ovarian homogenates compared to control and sham groups. The controlled reperfusion groups had a statistically significant lower IMA in serum compared to simple reperfusion groups. IMA was found to be higher in the ovarian homogenates of simple reperfusion compared to controlled reperfusion groups.Conclusion: Our results suggest that controlled reperfusion prevent the processes that increase the IMA in ovarian torsion.

Ischemia modified albumin in experimental ovarian torsion with and without controlled reperfusion

Biochemistry and Clinical Biochemistry Department

Nicolae Testemitanu State University of Medicine and Pharmacy

Surgery nr.1 “Nicolae Anestiadi” Department

Department of Surgical Gynecology, Mother and Child Institute

Revista Romana de Medicina de Laborator

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Purpose: Ovarian torsion, being a gynecological emergency, requires to be rapidly diagnosed and treated with minimal consequences on ovarian function after the...

Ischemia modified albumin in experimental ovarian torsion with and without controlled reperfusion

Online veröffentlicht: 01. Feb. 2019

Seitenbereich: 43 - 50

Eingereicht: 08. Nov. 2018

Akzeptiert: 28. Dez. 2018

DOI: https://doi.org/10.2478/rrlm-2019-0008

Schlüsselwörter
ovarian torsion, ischemia modified albumin

© 2019 Cornelia Lazăr et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Ischemia modified albumin in experimental ovarian torsion with and without controlled reperfusion

Online veröffentlicht: 01. Feb. 2019

Seitenbereich: 43 - 50

Eingereicht: 08. Nov. 2018

Akzeptiert: 28. Dez. 2018

DOI: https://doi.org/10.2478/rrlm-2019-0008

Schlüsselwörterovarian torsion, ischemia modified albumin

© 2019 Cornelia Lazăr et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Schlüsselwörter
ovarian torsion, ischemia modified albumin