<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Background To evaluate the effects of miR-99a on the migration and proliferation of glioma cells. Materials and Methods: Glioma cell line LN229 with stable up-regulation of miR-99a was constructed by transfection of hsa-miR-99a mimics, and cells with stable miR-99a knock-down were established by transfection of hsa-miR-99a inhibitor. The proliferation capacities of two groups were detected by the MTT assay, and their migration capacities were detected by the scratch assay. Results: LN229 cells with stable up-regulation and knock-down of miR-99a were successfully constructed. Up-regulating miR-99a inhibited the proliferation and migration of glioma cells, but knocking down this gene promoted their proliferation and migration. Conclusion: MiR-99a significantly affected the proliferation and migration of glioma cells, as a potentially eligible target for glioma therapy.</p></abstract>

Background To evaluate the effects of miR-99a on the migration and proliferation of glioma cells. Materials and Methods: Glioma cell line LN229 with stable up-regulation of miR-99a was constructed by transfection of hsa-miR-99a mimics, and cells with stable miR-99a knock-down were established by transfection of hsa-miR-99a inhibitor. The proliferation capacities of two groups were detected by the MTT assay, and their migration capacities were detected by the scratch assay. Results: LN229 cells with stable up-regulation and knock-down of miR-99a were successfully constructed. Up-regulating miR-99a inhibited the proliferation and migration of glioma cells, but knocking down this gene promoted their proliferation and migration. Conclusion: MiR-99a significantly affected the proliferation and migration of glioma cells, as a potentially eligible target for glioma therapy.

Effects of miR-99a on the migration and proliferation of glioma cells

Revista Romana de Medicina de Laborator

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

{"article-title":"Effects of miR-99a on the migration and proliferation of glioma cells"}

Background To evaluate the effects of miR-99a on the migration and proliferation of glioma cells. Materials and Methods: Glioma cell line LN229 with stable...

Effects of miR-99a on the migration and proliferation of glioma cells

Online veröffentlicht: 17. Mai 2018

Seitenbereich: 145 - 152

Eingereicht: 21. Aug. 2017

Akzeptiert: 10. Jan. 2018

DOI: https://doi.org/10.2478/rrlm-2018-0005

Schlüsselwörter
miR-99a, migration, proliferation, glioma

© by Weibang Liang

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Effects of miR-99a on the migration and proliferation of glioma cells

Online veröffentlicht: 17. Mai 2018

Seitenbereich: 145 - 152

Eingereicht: 21. Aug. 2017

Akzeptiert: 10. Jan. 2018

DOI: https://doi.org/10.2478/rrlm-2018-0005

SchlüsselwörtermiR-99a, migration, proliferation, glioma

© by Weibang Liang

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Schlüsselwörter
miR-99a, migration, proliferation, glioma