<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec><h3>Background</h3>
<p>Gastric cancer is an epidemic malignancy that is commonly diagnosed at the late stage. Evidence has elucidated that RAD54B exerts a crucial role in the progress of various tumors, but its specific role and mechanism in gastric cancer remain gloomy.</p>
</sec>
<sec><h3>Materials and methods</h3>
<p>The level of RAD54B was detected by western blot. RAD54B expression was downregulated or upregulated in both MKN45 and AGS cells by the transfection of shRAD54B or overexpression plasmid, respectively. The role of RAD54B in the growth, migration, invasion and tube formation of gastric cancer was evaluated by Edu, colony formation, transwell and tube formation assays. In addition, the molecular mechanism of RAD54B in gastric cancer was also determined by western blot. Moreover, <italic>in vivo</italic> experiment was conducted in xenografted mice.</p>
</sec>
<sec><h3>Results</h3>
<p>The expression of RAD54B was discovered to be upregulated in gastric cancer based on the ATGC and GEPIA databases, which was also confirmed in gastric cancer cell lines. Moreover, overexpression of RAD54B enhanced the growth, migration, invasion, tube formation and Wnt/β-catenin signaling axis in AGS and MKN45 cells. As expected, knockdown of RAD54B in AGS and MKN45 cells reversed these promotions. More importantly, <italic>in vivo</italic> assay also verified that RAD54B accelerated the growth of gastric cancer and Wnt/β-catenin signaling pathway.</p>
</sec>
<sec><h3>Conclusions</h3>
<p>Both loss-of-function and gain-of-function assays demonstrated that RAD54B facilitated gastric cancer cell progress and angiogenesis through the Wnt/β-catenin axis.</p>
</sec>
</abstract>

Background
Gastric cancer is an epidemic malignancy that is commonly diagnosed at the late stage. Evidence has elucidated that RAD54B exerts a crucial role in the progress of various tumors, but its specific role and mechanism in gastric cancer remain gloomy.

Materials and methods
The level of RAD54B was detected by western blot. RAD54B expression was downregulated or upregulated in both MKN45 and AGS cells by the transfection of shRAD54B or overexpression plasmid, respectively. The role of RAD54B in the growth, migration, invasion and tube formation of gastric cancer was evaluated by Edu, colony formation, transwell and tube formation assays. In addition, the molecular mechanism of RAD54B in gastric cancer was also determined by western blot. Moreover, in vivo experiment was conducted in xenografted mice.

Results
The expression of RAD54B was discovered to be upregulated in gastric cancer based on the ATGC and GEPIA databases, which was also confirmed in gastric cancer cell lines. Moreover, overexpression of RAD54B enhanced the growth, migration, invasion, tube formation and Wnt/β-catenin signaling axis in AGS and MKN45 cells. As expected, knockdown of RAD54B in AGS and MKN45 cells reversed these promotions. More importantly, in vivo assay also verified that RAD54B accelerated the growth of gastric cancer and Wnt/β-catenin signaling pathway.

Conclusions
Both loss-of-function and gain-of-function assays demonstrated that RAD54B facilitated gastric cancer cell progress and angiogenesis through the Wnt/β-catenin axis.

RAD54B promotes gastric cancer cell migration and angiogenesis via the Wnt/β-catenin pathway

Radiology and Oncology

This work is licensed under the Creative Commons Attribution 4.0 International License.

Background
Gastric cancer is an epidemic malignancy that is commonly diagnosed at the late stage. Evidence has elucidated that RAD54B exerts a crucial role in...

RAD54B promotes gastric cancer cell migration and angiogenesis via the Wnt/β-catenin pathway

Article Category: Research Article

Online veröffentlicht: 21. Feb. 2024

Seitenbereich: 67 - 77

Eingereicht: 28. Juli 2023

Akzeptiert: 06. Nov. 2023

DOI: https://doi.org/10.2478/raon-2024-0007

Schlüsselwörter
gastric cancer, RAD54B, migration, angiogenesis, Wnt/β-catenin

© 2024 Jianchao Li et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

RAD54B promotes gastric cancer cell migration and angiogenesis via the Wnt/β-catenin pathway

Article Category: Research Article

Online veröffentlicht: 21. Feb. 2024

Seitenbereich: 67 - 77

Eingereicht: 28. Juli 2023

Akzeptiert: 06. Nov. 2023

DOI: https://doi.org/10.2478/raon-2024-0007

Schlüsselwörtergastric cancer, RAD54B, migration, angiogenesis, Wnt/β-catenin

© 2024 Jianchao Li et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Schlüsselwörter
gastric cancer, RAD54B, migration, angiogenesis, Wnt/β-catenin