Cancer remains a major public health concern worldwide and is the second leading cause of death in Europe and the United States, with one-in-two to one-in-three chance to develop an invasive cancer during individual’s lifetime.1 In Slovenia, cancer caused over 35 % deaths in males in 2016, which is the highest share in European Union.2 As a result of positive lifestyle changes and advances in tumour detection and treatment, we can observe a continuous drop in mortality rates in the last 20 years.3 Despite, current treatments like chemotherapy, surgery and radiation commonly have debilitating side effects. Consequently, new therapeutic options are becoming available to curb the tremendous death toll and increase the quality of life for cancer survivors.4 In this review we will focus on cancer therapeutics in form of viral gene therapy vectors and oncolytic viruses (OVs).
Viral vectors are an attractive drug delivery option due to their evolved efficiency to transduce human cells. Compared to other delivery methods, viruses are also easier to use in targeted transfer of genetic cargo. That is why modified viruses are used as reliable and safe gene therapy vectors in cancer and hereditary disease treatment.5 However, early attempts at viral gene therapy came too soon for the budding technology, which lead to controversy and poor public image. For example, the initial trials for severe combined immunodeficiency (SCID) saw only limited improvement and adeno-virus vector-associated complications lead to tragic death of Jesse Gelsinger in 1999.6 The tides turned in later years, when viral vectors were successfully used as
Oncolytic therapy represents another use of viral vectors. It was sparked by serendipitous observations of transient remissions when cancer patients contracted viral infections.12 This led to experimentation with natural pathogens to help cure tumours, mostly with little success. Although initial attempts of viral oncolytic therapy were ineffective, the genetic engineering revolution enabled development of effective OVs in 1990s.13,14 Thirty years later, three oncolytic therapeutics are approved for use, with many more entering the clinics. Overall, oncolytic and viral gene delivery vectors have great potential to complement established (immuno)therapy approaches. These advanced nanotherapeutics are armed with a wide variety of genetic elements that take advantage of essential hallmarks of cancer, harnessing the accumulated knowledge in cancer biology, immunology and virology. Examples of therapeutic viral vector platforms, their opportunities and challenges are discussed below.
At present, over 1000 clinical trials for cancer therapy with viral vectors are underway worldwide (Figure 1).11 The use of virus particles in cancer treatment can be broadly classified in two groups: as gene delivery vehicles and OVs.15,16 For gene delivery, lentiviral, adenoviral and AAV vector chas
sis are used – depending on the specific application and targeting specificity. OVs encompass many viral families and are often additionally armed to eradicate the tumour and induce anti-cancer immune response.4 The main difference between vectors in gene- and oncolytic therapy is their replicative potential. Gene therapy vectors are specifically engineered to prevent replication. Consequently, they function as nanoparticle drug delivery vehicles and cannot actively infect host cells. In contrast, OVs are less attenuated and can replicate in infected tissues. Each of these vector designs has its own set of advantages and disadvantages, which also depend on the clinical indication. Below, we overview some of the most widely used viral vectors for cancer treatment that were either approved for clinical use or introduce exciting new concepts for future therapies.
Gene delivery vectors are used to transfer therapeutic genetic material to target tissues. In cancer therapy this includes tumour suppressor genes, tumour-associated antigens (TAAs), pro-inflammatory factors, immune checkpoint inhibitors, anti-angiogenic proteins, small interference RNA (siRNA), cancer stroma-degrading enzymes and cytotoxic convertases.17 In addition, vector gene delivery is used to reprogramme therapeutic cells
Native adenoviruses are 90 nm icosahedral particles (Figure 2A) that commonly cause respiratory, gastrointestinal, urinary and keratoconjunctivitis infections in humans. Their ubiquity results in high proportion of life-long immunity in human population towards the most common serotypes.20 Adenoviruses have 36 kilobase pair (kbp) linear double-stranded DNA (dsDNA) genome consisting of over 30 genes that are flanked by inverted terminal repeats (ITRs) and a capsid-packing signal sequence ψ. The adenoviral genes are divided into early (E) and late (L) genes, depending on their expression pattern. Early expressed regulatory proteins interact with the host cell and initiate viral genome replication. On the other hand, late genes encode structural proteins that form the virion.21 Adenoviral vectors were developed by deleting key regulatory genes, which depend on the desired transgene size and application (Figure 2B). Replication-competent adenovirus vectors are used in oncolytic cancer therapy, while non-replicative deletion mutants are gene delivery vehicles. In the first generation of adenovirus vectors, the essential early E1A and E1B genes are replaced by constitutive expression cassette with transgene for gene delivery. Additionally, E2, E3 and E4 genes can also be deleted to accommodate larger therapeutic inserts of 10 kbp and improve performance. For viral vector assembly, the modified adenovirus genome is expressed from plasmid DNA in human embryonic kidney (HEK) 293 cell line that complements for deleted E1, E2 and E4 genes. Lastly, as much as 36 kbp inserts can be accommodated in helper-dependent adenovirus vectors that retain only ITR and genome packaging sequence ψ, rest is filled with one or several transgene expression cassettes. All adenoviral proteins needed for vector replication, packaging and assembly are provided by the replication-competent helper virus, which has its packaging signal flanked by loxP recombination sites. The helper-dependent vector production takes place in cell lines expressing Cre recombinase that specifically excises the loxP-flanked helper ψ sequence. This ensures only the transgene vector retains the ψ packaging signal and is incorporated in the budding viral particles. Remaining helper virus contaminants are eliminated in the following chromatography purification process. Adenoviral vectors have broad tropism and do not integrate into target cell genome. Instead, the delivered genetic material remains episomal.15,22
In gene delivery, acquired and innate immunity towards adenovirus vectors is hindering their application. For example, the most widely used Ad5 serotype has 50% seroprevalence in North America and over 90% in Côte d’Ivoire.15 Additionally, the adenoviral capsid and nucleic acid stimulates components of the complement system and Toll-like receptors (TLR). This raises safety concerns and efficacy issues for systemic adenoviral gene delivery
In 2003, the adenovirus-based Gendicine became the first registered cancer gene therapy treatment. Gendicine is an E1- and E3-deletion Ad5 viral vector, which encodes tumour suppressor p53 under Rous sarcoma virus (RSV) promoter regulation. The loss of p53 protective function is associated with at least half of cancers.23 Once the Ad5 vector delivers p53 transgene, it resumes anti-tumour function by promoting cancer cell apoptosis and stimulating the immune response. It received approval in China for advanced head and neck cancer treatment.23,24
Adenovirus vectors are also used to deliver cancer suicide genes that convert prodrugs to cytotoxic compounds. Examples include 5-fluorouridine (5-FU)-producing cytosine deaminase, purine nucleoside phosphorylase (PNP) that converts fludarabine phosphate (F-ara-AMP) to toxic 2-fluoroadenine, and ganciclovir-converting thymidine kinase (TK).15 Sitimagene ceradenovec (Cerepro, Ark Therapeutics) is a first-generation Ad5 vector that expresses convertase from herpes virus HSV-TK. In 2005 it entered phase 3 trial for treatment of glioblastoma. In the trial, 1·1012 Ad5 vector particles were applied locally into the resected tumour and ganciclovir was administered intravenously. The study found no effect on survival, while the viral vector treatment improved time to re-intervention or death after resection – the primary trial endpoints.25 Despite this, the Cerepro marketing application in Europe was withdrawn in 2010.26
Similarly, immunostimulatory adenovirus cancer gene therapy was used to promote interferon alpha and beta (IFNα, -β), interleukin 2 (IL-2) and Fms-like tyrosine kinase 3 ligand (Flt3L) expression.20 A phase 3 trial for bacille Calmette-Guérin (BCG)-unresponsive bladder cancer with nadofaragene firadenovec, a replication deficient vector expressing IFNα, recently reported favourable results.27 The non-muscle-invasive and BCG-unresponsive bladder cancer currently does not have efficient non-surgical treatments, which are often the only option for many patients. Adenoviral gene therapy is a promising alternative, since local administration led to 60- and 30% complete response rate after 3 and 12 months, respectively.28 Lastly, the engineered chimpanzee ChAdOx1 vector vaccine platform – also used by the AZD1222 COVID-19 vaccine (Vaxzevria, Oxford-AstraZeneca) – is aimed at prostate cancer treatment in combination with checkpoint inhibitors.10,29,30 The cancer vaccine treatment consists of ChAdOx1 immunization against 5T4 tumour antigen and a Modified Vaccinia Ankara (MVA) vector boost. The phase 1 trial confirmed vaccine safety and immunogenicity, while phase 1/2 efficacy trial was expected to complete in 2021.31 Overall, adenoviral gene delivery remains a promising venue for cancer therapy, either alone or in combination with radiotherapy, chemotherapy or checkpoint inhibitors.32 Also, the ease of industrial scale-up and established Good Manufacturing Practice (GMP) processes will further promote adenoviral platform for
Adeno-associated viruses (AAVs) hold great promise in the gene therapy field. AAVs do not cause any human disease, are non-replicative and have broad tissue tropism. AAVs are 25 nm icosahedral viruses (Figure 3A) with single-stranded DNA (ssDNA) genome, which naturally lacks many key regulatory genes for replication and expression. The missing genes are instead complemented with adenoviral co-infection of the host cell. Alternatively, herpes simplex and baculovirus can also provide the helper function. For gene delivery, the AAV genome is “gutted”—devoid of all viral genes – and replaced with transgene expression cassette (Figure 3B). The major AAV vector downside is its relatively low capacity for transgene inserts – it can accommodate 4.7 kbp of genetic cargo, which can be limiting for many applications.15,34 The therapeutic AAV particles are commonly produced from three plasmid constructs in transfected HEK 293 cells, which already encode the adenoviral E1 helper gene. The vector plasmids contain the ITR-flanked transgene, AAV rep and cap genes and the adenoviral E2, E4 and VA genes, respectively. A more scalable solution is possible with Sf9 insect cells, which are co-infected with ITR-transgene and AAV cap/rep baculoviruses, respectively.35
Different AAV serotypes display distinct tropism, but they generally require AAV receptor (AAVR) expression, heparin sulphate peptidoglycans, sialic acid or galactose with several co-receptors for cell transduction.36 Once the vector enters the cell, it travels to nucleus and uncoats the transgene DNA, which persists as concatemerized episomal circle for many years.35 Currently, AAV vectors are the most successful in treatment of monogenic diseases like spinal muscle atrophy (SMA) with onasemnogene abeparvovec (Zolgensma, Novartis).37 In contrast, AAV-based cancer therapies are still in early development. However, the modular vector design enables new promising approaches to targeted gene delivery.38
For instance, AAVs that cross the blood-brain barrier and are specific for central nervous system could be used for treatment of invasive glioblastoma.39 To improve cancer specificity, wild-type AAV capsids can also be engineered to target cell surface tumour antigens.40,41 For example, AAV2 was modified to bind HER2 receptor by inserting designed ankyrin repeat proteins (DARPins) into the AAV capsid.42 The researches later used these Her2-AAVs to specifically deliver checkpoint inhibitors against programmed cell death protein-1 (PD-1) and HSV-TK suicide gene in a mice xenograft model.43,44 A single systemic injection of Her2-AAV vector, armed with HSV-TK, lead to considerable tumour mass reduction in combination with ganciclovir.44 On the other hand, PD-1 inhibition lead to only marginal tumour clearance in combination with chemotherapy.43 In an
Overall, AAV particles are less immunogenic compared to other vector types, although majority of adults have pre-existing neutralizing antibodies that can affect AAV-based gene therapy efficency.51 Also, AAVs are regarded as very safe due to their non-toxic nature and expected lack of genome integration. However, a recent long-term study of AAV-treated dogs with haemophilia raised concerns as numerous vector integration events were surprisingly discovered in vicinity of cancer-associated genes.52 Nonetheless, the superior versatility makes AAVs currently the up-and-coming method for gene delivery
While adenovirus and AAV vectors are predominantly used to deliver gene drugs
In cancer therapy, lentiviral vectors are most used for
Gammaretrovirus vectors, which are derived from murine leukaemia virus (MLV), are another type of retroviral vectors. In contrast to lentivirus, gammaretrovirus vector infects only actively dividing cells and is prone to integrate into gene regulatory regions, raising concerns for insertional oncogenesis.56 Nevertheless, axicabtagen ciloleucel (Yescarta, Gilead) is a CD19 CAR-T cell therapy for diffuse large B-cell lymphoma, which utilizes gammaretroviral vector for chimeric receptor gene delivery.66 Another gammaretroviral therapeutic is vocimagene amiretrorepvec (Toca 511), which encodes yeast cytosine deaminase that converts prodrug 5-fluorocytosine to toxic 5-FU in glioma cells.67 Despite promising results from mouse brain tumour models, a phase 2/3 did not show improved patient survival compared to standard-of-care after Toca 511 injection.68 Lenti- and gammaretroviral vectors will remain the method of choice for
In contrast to viral vectors for gene therapy, engineered OVs are replication-competent and more closely resemble their natural counterparts. In fact, tumour regressions after natural viral infections have been reported since the end of 19th century.13 The OV therapy takes advantage of rapidly dividing cancer cells, which often lack antiviral defence mechanisms present in normal cells. For example, misregulation of interferon, Wnt, Ras/MAPK, p53 and pRb signalling pathways leaves cancer vulnerable for viral infection.70,71 Consequently, OVs preferentially replicate in cancerous cells, resulting in lysis, tumour eradication and immune system engagement. Besides the viral tumour debulking, the immunostimulatory effect is especially important in “cold” tumours with few infiltrating lymphocytes, inhibitory tumour microenvironment (TME) and impaired antigen presentation. There, viral antigens, danger-associated molecular patterns (DAMPs), TAAs and neoantigens are released to TME during infected tumour cell lysis. Released factors are then presented to the innate and adaptive immune system, acting as self-adjuvating
Replication-competent adenovirus vectors were the first oncolytic viruses to reach clinical trials in 1998 with ONYX-015.78 This adenoviral vector harbours E1B-55K deletion, which attenuates viral replication in normal cells. While ONYX-015 presented a remarkable safety profile, it conferred only limited responses in combination with chemotherapy for many different cancers.22 In 2005, a similar vector H101 (Oncorine) became the first approved oncolytic virotherapy for late-stage nasopharyngeal carcinoma treatment in China. The objective response rate of Oncorine in combination with chemotherapy was 76% versus 59% for chemotherapy alone in phase 3 trial.79 Altogether, hundreds of patients received Oncorine, which was well tolerated and without adverse side effects.22
It took 10 years for another OV to be approved by the FDA and EMA in 2015. T-VEC or talimogene laherparepvec (Imlgyc, Amgen) is an oncolytic herpesvirus that expresses immunomodulatory granulocyte macrophage colony-stimulating factor (GM-CSF) for advanced melanoma treatment.80 Non-modified herpes simplex virus type 1 (HSV-1) is a neurotropic human pathogen with 152 kbp dsDNA genome and about 90 genes. For cancer therapy with T-VEC vector, HSV-1 from a clinical isolate was modified with infected cell protein 34.5 (ICP34.5) deletions, preventing virus replication in neurons and other slowly replicating cells. Conversely, this deletion increases HSV-1 replication specificity for tumour cells, while another deletion in ICP47 increases viral and tumour antigen presentation. Lastly, T-VEC is armed with two copies of GM-CSF to further promote activation of local antigen-presenting cells.72,81 The virotherapy proved effective in phase 3 trial where 16% of patients showed durable response compared to 2% for GM-CSF treatment. The overall response rate was 26% for T-VEC and 6% for GM-CSF alone. Furthermore, 64% of injected lesions more than halved in size, together with 34% and 15% of distal uninjected regional and visceral lesions, respectively.82 A similar antitumour T-VEC activity was recently reported for primary cutaneous B cell lymphoma. Interestingly, this phase 1 study also showed therapeutic virus replication in non-malignant cells and determined that induced immunological responses are more important in cancer eradication than selective viral cell lysis.83,84 Currently, combination therapy studies with T-VEC are underway for different cancers.16,85,86 A similar set of HSV-1 attenuations is also present in G47Δ or teserpaturev (Delytact, Daiichi Sankyo), which is derived from a different parental strain. In 2021, it received conditional authorization for malignant glioma therapy in Japan.87 In contrast to T-VEC, which is armed with GM-CSF, Delytact does not encode any transgenes.88
The GM-CSF cytokine sequence is also loaded in oncolytic vaccinia virus pexastimogene devacirepvec (JX-594, Pexa-Vec) to promote
The complexity of viral vector genomes still poses a potential risk of recombination and acquired pathogenicity during vector production and therapy. Indeed, with an increasing number of effective OVs reaching late clinical trials and regulatory approvals, a particular care is given to biosafety monitoring and interaction of replication-competent vectors with the host and environment. Based on gathered experience, engineered oncolytic viral vectors remain a safe and promising venue for cancer treatment. The most common reported side effects are mild flu-like symptoms, while there was no documented uncontrolled transmission of the oncolytic virus.14 It is becoming clear that OVs offer unique benefits in tumour immunotherapy, particularly in combination with advanced cell therapies, chemotherapy and checkpoint inhibitors.96
Reliable production platforms are key to successful translation of viral vector therapies into clinics. Indeed, the development of robust manufacturing capabilities enabled the widespread adoption of recombinant biotherapeutics like monoclonal antibodies. Compared to proteins, viral vectors are orders of magnitude more complex, where minute changes between serotypes can affect production and purification strategies. What is more, conventional protein purification methods are often not appropriate for shear-sensitive viral particle isolation. Enveloped vectors are easily ruptured during the purification process, which decreases the ratio of functional infectious particles in the final product. Consequently, it is no surprise that viral vector production platforms are continuously being optimized and are yet to reach their full potential on the industrial scale.6,97 Moreover, viral vectors for gene delivery and oncolysis span several viral families and are further genetically engineered to ensure safety and anti-tumoral potencies, adding to the diversity. This offers exciting opportunities for tailored therapies, but also raises challenges in manufacturing and regulation. Thus, paths to viral vector platform success and adoption are specific for each therapeutic.
In order to support consistent production and reliable scale-up to clinical-grade drug manufacture, an extra care has to be taken in initial selection of viral vector production platforms. Established mammalian cell cultures are most widely used for vector propagation. This makes sense since therapeutic viral vectors originate from natural viruses that co-evolved with vertebrate hosts. The producer cell lines include African green monkey Vero cells, human HEK 293 and HeLa cells and baby hamster kidney (BHK) line.98 They are handled under BSL-2 regulations to limit vector dissemination and contamination with adventitious agents.99 Mammalian cell lines are primarily adherent – growing attached to a solid support. For production, adherent cells are grown on microcarriers in bioreactors, on multilayer tissue plates and roller bottles. However, suspension cell cultures are preferred for easier scale-up. Luckily, many adherent cell lines were successfully adapted for suspension growth in stirred-tank and rocking Wave bioreactors.97,98,100, For example, the adenovirus-transformed HEK 293 cells are easily adapted for suspension culture and are the most widely used cell line for adenoviral and AAV vector production.
Vector components and helper constructs are delivered to the selected producer cells with transient transfection or infection, which represent a bottleneck and a significant expense in viral vector production for clinical trials.101 In principle, stable cell lines overcome transfection issues in large scale production. However, assembled vector components are often toxic for the host cell.5 This is why stable lentiviral production lines rely on inducible vector expression using TetON/OFF system.102 Alternatively, the cytotoxic VSV-G envelope protein and HIV-1 protease can be swapped with Ampho MLV 4070A glycoprotein and T26S modified protease to generate stable lentiviral vector producers LentiPro26 from HEK 293T cells.103 Besides mammalian cell cultures, insect cells are also increasingly used for vector assembly. Most commonly, Sf9 or HighFiveTM insect cell lines are grown in suspension where therapeutic vector backbones are introduced through infection with recombinant baculoviruses. Insect cell expression system is fast to implement, scalable and has superior safety profile since insects are poor hosts for human pathogens. So far, insect cells are used to produce helper-free AAV vectors for gene therapy.104,105 Like with mammalian viral vector systems, insect cells are grown in single-use cell culture flasks and bioreactors to ensure reproducibility and sterility during manufacture for clinical use.101,106
Viral vector purification platforms encompass purification steps to generate highly pure vector particles that comply with stringent quality, safety and efficacy standards. Historically, purification relied on ultracentrifugation to separate the large viral particles from smaller producer cell contaminants. Nonetheless, this approach is not scalable and does not guarantee elimination of biophysically similar particles.107 Instead, chromatography-based purification processes are taking the centre stage.108 Due to diversity of viral vectors – ranging from small 25 nm AAV particles to large enveloped vaccinia vector, which exceeds 300 nm in size – a universal purification process does not exist and has to optimized for each application. Generally, the purification process starts with vector particle harvesting, where secreted vectors like lentiviruses, herpes and poxviruses are separated from cellular debris with centrifugation or filtration, while intracellular adenoviral and AAV particles often require cell lysis for release. Then, the collected harvest is extensively purified over a series of different chromatography columns and tangential-flow filtration (TFF) cassettes to obtain pure therapeutic vector particles. In the end, the purified cancer drug is exchanged to the final formulation solution and sterile filtered through 0.2 μm pores, which can be problematic for some larger vectors. Instead, the entire production can be operated under controlled sterile conditions.97,108 The final dose of vector particles varies from 109 for
With many viral vector therapeutics reaching final clinical stage and regulatory approvals, the attention is focused on vector particle production platforms that support scalable industrial scale production. This is essential to bring down the cost of therapies, which is often prohibitive. New biotechnological solutions like gene editing, bioreactor cultivation and multimodal chromatography are boosting cell-based productivity and improving particle purity. Novel analytical methods are also improving the quality monitoring of the final product. In gene therapy, great emphasis is given to ensuring a high ratio of functional infectious vector particles versus defective and empty capsid contaminants.112 These exciting developments are enabling the viral vector platforms to produce safe and potent drugs to combat cancer – as mono-therapies or in combination with other therapeutic venues.
Viral vectors represent 100% of approved gene therapeutics and over 60% of delivery devices in gene therapy trials, including oncolytic virotherapy.11 This accumulation of knowledge helps us identify where vector particles can provide the most benefits. Based on recent success and rapid advances in the field, the clinical viral vector use will continue to increase. Particularly, combination therapy with complementary radiotherapy, chemotherapy and immunotherapies like CAR-T and checkpoint inhibitors hold great promise. Due to modular vector genome design, novel biotherapeutics like BiTEs, cytokines and CRISPR/Cas can be encoded in the genetic cargo to expand the repertoire of anti-tumour potency.113 In addition to new vector development, approved viral drugs like T-VEC are being tested to treat several other solid tumours beyond melanoma. Finally, oncolytic viruses and non-replicative vectors can be used in prime-boost cancer vaccine regimens, covering the full spectrum of the discussed vector platforms.114
With obvious benefits to the viral vector oncotherapy, these engineered nanotherapeutics will continue to expand the cancer treatment repertoire. It seems like viral vector platforms are finally living up the high expectations thanks to the advances in biopharmaceutical manufacturing and our understanding of cancer and viral biology. In the future, many more vector particles will enter the clinics. Excitingly, their adaptable design will enable