<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>An established biotesting system designed to discover specific features of innovative multifunctional biotextile, encompassing integrated silica dioxide and succinite (Baltic amber) particles, is founded on reproducible, reliable, and relatively fast methods. The main idea starting this study was to create a system of test methods devoted to identification of specific features of biotextile materials designed to preserve living organisms from adverse environmental factors like enhanced electromagnetic radiation of different frequencies, without use of vivarium animals. Cultures of the freshwater macrophyte duckweed (<italic>Lemna minor</italic>) line <italic>Sta2</italic> and fruit flies (<italic>Drosophila melanogaster</italic>) were chosen as model systems suitable for the study of the influence of electromagnetic field (EMF) radiation. The experiments showed changes of phenotypic features and growth parameters of test objects, as well as induction of point mutations of DNA (for example, insertions or deletions in chloroplast DNA and nucleotide substitutions in nuclear genes). The responses of test organisms induced by EMF were studied using microscopy, flow cytometry, and DNA sequencing methods. On the cell level, a new fast flow cytometry method for biotextile testing was developed: immature gametic (pollen) cell cultures were used as a highly sensitive model system (plant gametic cell response is comparable to human neutrophil response) to study the influence of EMF radiation. The method was based on the measurement of differences of fluorescence intensity between group of cells experimentally affected by EMF radiation and non-affected cells.</p>
</abstract>

An established biotesting system designed to discover specific features of innovative multifunctional biotextile, encompassing integrated silica dioxide and succinite (Baltic amber) particles, is founded on reproducible, reliable, and relatively fast methods. The main idea starting this study was to create a system of test methods devoted to identification of specific features of biotextile materials designed to preserve living organisms from adverse environmental factors like enhanced electromagnetic radiation of different frequencies, without use of vivarium animals. Cultures of the freshwater macrophyte duckweed (Lemna minor) line Sta2 and fruit flies (Drosophila melanogaster) were chosen as model systems suitable for the study of the influence of electromagnetic field (EMF) radiation. The experiments showed changes of phenotypic features and growth parameters of test objects, as well as induction of point mutations of DNA (for example, insertions or deletions in chloroplast DNA and nucleotide substitutions in nuclear genes). The responses of test organisms induced by EMF were studied using microscopy, flow cytometry, and DNA sequencing methods. On the cell level, a new fast flow cytometry method for biotextile testing was developed: immature gametic (pollen) cell cultures were used as a highly sensitive model system (plant gametic cell response is comparable to human neutrophil response) to study the influence of EMF radiation. The method was based on the measurement of differences of fluorescence intensity between group of cells experimentally affected by EMF radiation and non-affected cells.

Establishment of Biotesting System to Study Features of Innovative Multifunctional Biotextile

Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.

This work is licensed under the Creative Commons Attribution 4.0 International License.

An established biotesting system designed to discover specific features of innovative multifunctional biotextile, encompassing integrated silica dioxide and...

Establishment of Biotesting System to Study Features of Innovative Multifunctional Biotextile

Online veröffentlicht: 28. Sept. 2023

Seitenbereich: 186 - 192

Eingereicht: 06. Juli 2023

Akzeptiert: 15. Aug. 2023

DOI: https://doi.org/10.2478/prolas-2023-0026

Schlüsselwörter
silica dioxide nanoparticles, electromagnetic field (EMF) radiation, model organisms

© 2023 Dace Grauda et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Establishment of Biotesting System to Study Features of Innovative Multifunctional Biotextile

Online veröffentlicht: 28. Sept. 2023

Seitenbereich: 186 - 192

Eingereicht: 06. Juli 2023

Akzeptiert: 15. Aug. 2023

DOI: https://doi.org/10.2478/prolas-2023-0026

Schlüsselwörtersilica dioxide nanoparticles, electromagnetic field (EMF) radiation, model organisms

© 2023 Dace Grauda et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Schlüsselwörter
silica dioxide nanoparticles, electromagnetic field (EMF) radiation, model organisms