<abstract xmlns="http://www.w3.org/1999/xhtml"><italic>Prunus necrotic ring spot ilarvirus (PNRSV)</italic> and <italic>Apple chlorotic leaf spot trichovirus (ACLSV)</italic> are common in plum orchards. The aim of the study was to obtain virus-free planting material of <italic>Prunus domestica</italic> L. by chemotherapy <italic>in vitro</italic>. Ribavirin at concentrations of 10 to 50 mg·l−1 was added to Murashige–Skoog (MS) nutrition medium for virus eradication from microshoots. After a two-week period of chemotherapy, meristems were subcultured monthly on MS medium and proliferation index of shoots was estimated. Microshoots were retested by reverse transcription polymerase chain reaction for presence of virus. At lowest concentrations of 10 mg·l−1 ribavirin was entirely ineffective for <italic>ACLSV</italic> and 10 to 30 mg·l−1 was ineffective for <italic>PNRSV</italic> elimination. Ribavirin concentrations of 40 and 50 mg·l−1 destroyed both pathogens. However, at higher concentrations of 40 and 50 mg·l−1 ribavirin exhibited some signs of phytotoxicity on microshoots in the first sub-cultivation period. In order to test the genetic stability of the microplants after chemotherapy the amplified fragment length polymorphism (AFLP) method was applied. Plant genome stability in ‘Magna Glauca’ at concentrations of 40 mg·l−1 was damaged, as the presence of polymorphic AFLP markers were observed.</abstract>

Prunus necrotic ring spot ilarvirus (PNRSV) and Apple chlorotic leaf spot trichovirus (ACLSV) are common in plum orchards. The aim of the study was to obtain virus-free planting material of Prunus domestica L. by chemotherapy in vitro. Ribavirin at concentrations of 10 to 50 mg·l−1 was added to Murashige–Skoog (MS) nutrition medium for virus eradication from microshoots. After a two-week period of chemotherapy, meristems were subcultured monthly on MS medium and proliferation index of shoots was estimated. Microshoots were retested by reverse transcription polymerase chain reaction for presence of virus. At lowest concentrations of 10 mg·l−1 ribavirin was entirely ineffective for ACLSV and 10 to 30 mg·l−1 was ineffective for PNRSV elimination. Ribavirin concentrations of 40 and 50 mg·l−1 destroyed both pathogens. However, at higher concentrations of 40 and 50 mg·l−1 ribavirin exhibited some signs of phytotoxicity on microshoots in the first sub-cultivation period. In order to test the genetic stability of the microplants after chemotherapy the amplified fragment length polymorphism (AFLP) method was applied. Plant genome stability in ‘Magna Glauca’ at concentrations of 40 mg·l−1 was damaged, as the presence of polymorphic AFLP markers were observed.

Influence of Ribavirin on <italic>Prunus Domestica</italic> L. Regeneration, Genome Stability and Virus Eradication <italic>In Vitro</italic>

Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry

Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Influence of Ribavirin on  L. Regeneration, Genome Stability and Virus Eradication 

Prunus necrotic ring spot ilarvirus (PNRSV) and Apple chlorotic leaf spot trichovirus (ACLSV) are common in plum orchards. The aim of the study was to obtain...

Influence of Ribavirin on Prunus Domestica L. Regeneration, Genome Stability and Virus Eradication In Vitro

Online veröffentlicht: 03. Juni 2019

Seitenbereich: 238 - 243

Eingereicht: 28. Jan. 2019

Akzeptiert: 24. März 2019

DOI: https://doi.org/10.2478/prolas-2019-0038

Schlüsselwörter
ACLSV, chemotherapy, PNRSV, virus free plant

© 2019 Ingrida Mazeikiene et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Influence of Ribavirin on Prunus Domestica L. Regeneration, Genome Stability and Virus Eradication In Vitro

Online veröffentlicht: 03. Juni 2019

Seitenbereich: 238 - 243

Eingereicht: 28. Jan. 2019

Akzeptiert: 24. März 2019

DOI: https://doi.org/10.2478/prolas-2019-0038

SchlüsselwörterACLSV, chemotherapy, PNRSV, virus free plant

© 2019 Ingrida Mazeikiene et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Schlüsselwörter
ACLSV, chemotherapy, PNRSV, virus free plant