Tunisian inland water microflora as a source of phycobiliproteins and biological activity with beneficial effects on human health
, , , , , und | 03. Dez. 2021
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Article Category: Original research paper
Online veröffentlicht: 03. Dez. 2021
Seitenbereich: 385 - 397
Eingereicht: 02. Mai 2021
Akzeptiert: 05. Juli 2021
DOI: https://doi.org/10.2478/oandhs-2021-0033
Schlüsselwörter
© 2021 Khaled Gharbi et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Figure 1
Molecular identification of microalgal strains isolated from different Tunisian inland water bodies with % of similarity according to the BLAST database and accession numbers
| Strain | Taxon according to BLAST database | % of similarity | Accession numbers | |
|---|---|---|---|---|
| 16S rRNA | rpoC1 | |||
| Osci-NB-01 | 99 | MG762090 | - | |
| Osci-BM-01 | 100 | MG762091 | - | |
| Plank-SS-01 | 100 | MG762092 | - | |
| Cyl-NB-05 | 96 | - | HQ389355 | |
| Pseud-01 | 99 | MG098078 | - | |
| Arthro-KB | 100 | MG098079 | - | |
| Lepto-CH | 99 | MG098077 | - | |
| Spir-ML | 99 | MG518486 | - | |
Phenolics, flavonoids and antioxidant activity of microalgal extracts
| Strain | Code | TPP (mg EqGA/g DW) | TF (mg EqC/g DW) | Antioxidant activity against DPPH (IC50, μg ml−1) |
|---|---|---|---|---|
| Chroo-CH | 8.90 ± 0.25 | 67.94 ± 1.36 | 212.15 ± 2.07 | |
| Osci-NB-01 | 1.81 ± 0.39 | 58.46 ± 0.73 | 263.91 ± 14.93 | |
| Arthro-KB | 2.85 ± 0.06 | 74.09 ± 5.28 | 953.94 ± 28.14 | |
| Lepto-CH | 7.75 ± 0.77 | 68.17 ± 3.96 | 782.87 ± 4.2 | |
| Plank-SS-01 | 4.86 ± 0.3 | 38.32 ± 5.37 | NA | |
| Pseud-01 | 5.63 ± 0.15 | 38.05 ± 4.43 | 912.21 ± 2.21 | |
| Cyl-NB-05 | ND | 1.48 ± 0.03 | NA | |
| Osci-BM-01 | 1.77 ± 0.13 | 36.31 ± 3.12 | NA | |
| Spir-ML | ND | 5.03 ± 1 | NA | |
| Duna-GV | 3.07 ± 0.32 | 37.94 ± 3.11 | 681 ± 20,07 | |
| BHT | - | - | - | 17.34 ± 0.23 |
Antileishmanial activity of microalgal extracts
| Strain | IC50 ± SD (μg ml−1) | |
|---|---|---|
| Chroo-CH | 713.4 ± 0.24 | 1000 ± 1.96 |
| Osci-NB-01 | 448.7 ± 0.24 | 728.5 ± 1.21 |
| Arthro-KB | > 1000 | > 1000 |
| Lepto-CH | 212.7 ± 1.46 | 401.9 ± 1.22 |
| Plank-SS-01 | 1000 ± 1.98 | > 1000 |
| Pseud-01 | 1000 ± 2.38 | > 1000 |
| Cyl-NB-05 | 246.4 ± 1.66 | 473.8 ± 0.53 |
| Osci-BM-01 | 188.7 ± 1.35 | 377.5 ± 0.09 |
| Spir-ML | 1000 ± 2.44 | > 1000 |
| Duna-GV | 151.2 ± 0.37 | 284.87 ± 1.46 |
| Amphotericin B | 0.64 ± 0.24 | 0.97 ± 0.08 |
Microalgal strains isolated from Tunisian inland water bodies (river, lagoon, dam reservoir, spring) with location and culture mediums
| Strain | Class/Order | Sampling site | Culture medium | |
|---|---|---|---|---|
| Duna-GV | Chlorophyceae/Chlamydomonadales | El Grine Sabkha |
CONWAY | |
| Chroo-CH | Cyanobacteria / Chroococcales | Chanchou River |
BG11 | |
| Arthro-KB | Cyanobacteria / Oscillatoriales | Korba lagoon |
BG11 | |
| Osci-NB-01 | Nabhena reservoir |
BG11 | ||
| Lepto-CH | Chanchou River |
BG11 | ||
| Osci-BM-01 | Bir Mcherga reservoir |
BG11 | ||
| Plank-SS-01 | Sidi Saad reservoir |
BG11 | ||
| Spir-ML | Maltine River |
CONWAY | ||
| Pseud-01 | Cyanobacteria / Nostocales | Siliana spring |
BG11 | |
| Cyl-NB-05 | Nabhena reservoir |
BG11 | ||
Antibacterial activity of microalgal extracts
| Strain | Inhibition zone diameter (mm) [MIC (μg ml−1)] | |||||
|---|---|---|---|---|---|---|
| MRSA | ||||||
| Chroo-CH | NA | NA | NA | NA | NA | NA |
| Osci-NB-01 | NA | NA | NA | NA | NA | NA |
| Arthro-KB | NA | NA | NA | NA | NA | NA |
| Lepto-CH | NA | NA | NA | NA | NA | NA |
| Plank-SS-01 | NA | NA | NA | NA | NA | NA |
| Pseud-01 | NA | NA | NA | NA | NA | NA |
| Cyl-NB-05 | NA | NA | NA | NA | NA | NA |
| Osci-BM-01 | NA | NA | NA | NA | NA | NA |
| Spir-ML | NA | NA | NA | NA | NA | NA |
| Duna-GV | NA | NA | NA | 16 ± 0.1 [375] | NA | NA |