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The effect of prenatal fumonisin B exposure on bone innervation in newborn Wistar rats

Ewa Tomaszewska

Ewa Tomaszewska

Department of Animal PhysiologyLublin, Poland
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Tomaszewska, Ewa
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Piotr Dobrowolski

Piotr Dobrowolski

Department of Functional Anatomy and Cytobiology, Faculty of Biology and Biotechnology, Maria Curie-Sklodowska UniversityLublin, Poland
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Dobrowolski, Piotr
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Aleksandra Dajnowska

Aleksandra Dajnowska

Department of Animal Anatomy and Histology, Faculty of Veterinary MedicineLublin, Poland
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Dajnowska, Aleksandra
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Liwia Arbatowska

Liwia Arbatowska

Department of Animal Anatomy and Histology, Faculty of Veterinary MedicineLublin, Poland
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Arbatowska, Liwia
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Iwona Puzio

Iwona Puzio

Department of Animal PhysiologyLublin, Poland
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Puzio, Iwona
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Halyna Rudyk

Halyna Rudyk

State Scientific Research Control Institute of Veterinary Medicinal Products and Feed AdditivesLviv, Ukraine
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Rudyk, Halyna
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Oksana Brezvyn

Oksana Brezvyn

State Scientific Research Control Institute of Veterinary Medicinal Products and Feed AdditivesLviv, Ukraine
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Brezvyn, Oksana
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Ihor Kotsyumbas

Ihor Kotsyumbas

State Scientific Research Control Institute of Veterinary Medicinal Products and Feed AdditivesLviv, Ukraine
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Kotsyumbas, Ihor
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Janine Donaldson

Janine Donaldson

School of Physiology, Faculty of Health Sciences, University of the WitwatersrandParktown, South Africa
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Donaldson, Janine
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Jadwiga Śliwa

Jadwiga Śliwa

Department of Animal Anatomy and Histology, Faculty of Veterinary MedicineLublin, Poland
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Śliwa, Jadwiga
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Marcin B. Arciszewski

Marcin B. Arciszewski

Department of Animal Anatomy and Histology, Faculty of Veterinary MedicineLublin, Poland
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Arciszewski, Marcin B.
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Siemowit Muszyński

Siemowit Muszyński

Department of Biophysics, Faculty of Environmental Biology, University of Life Sciences in LublinLublin, Poland
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Muszyński, Siemowit
  
09. Okt. 2024
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Online veröffentlicht: 09. Okt. 2024
Seitenbereich: 633 - 642
Eingereicht: 24. Apr. 2024
Akzeptiert: 23. Sept. 2024
DOI: https://doi.org/10.2478/jvetres-2024-0056
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© 2024 Ewa Tomaszewska et al., published by Sciendo
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Fig. 1.

A diagram showing the graphical analysis steps for image segmentation and bone neuronal net analysis. Red arrows indicate an example of an identified neuronal element in subsequent processes of image transformation
A diagram showing the graphical analysis steps for image segmentation and bone neuronal net analysis. Red arrows indicate an example of an identified neuronal element in subsequent processes of image transformation

Fig. 2.

Effects of fumonisin exposure on Wistar rat dams. a – post-parturition body weight; b – liver weight; c – relative liver weight (percentage of total body weight); d – serum aspartate aminotransferase (AST) activity; e – serum alanine aminotransferase (ALT) activity; f – litter size (number of live pups); (g) – offspring sex ratio (female-to-male); h – average pup body weight; i – female pup body weight; j – male pup body weight. Group I – control group; Group II – dams intoxicated with 60 fumonisins B (FB)/kg b.w. during gestation; Group III – dams intoxicated with 90 FB/kg b.w. during gestation. The data are presented as mean values ± standard error of the mean (n = 6), except for (f), where the line indicates the median, the box represents the interquartile range, and the whiskers show minimum and maximum value ranges. * – statistical significance at P-value < 0.05; ** – P-value < 0.01; *** – P-value < 0.001
Effects of fumonisin exposure on Wistar rat dams. a – post-parturition body weight; b – liver weight; c – relative liver weight (percentage of total body weight); d – serum aspartate aminotransferase (AST) activity; e – serum alanine aminotransferase (ALT) activity; f – litter size (number of live pups); (g) – offspring sex ratio (female-to-male); h – average pup body weight; i – female pup body weight; j – male pup body weight. Group I – control group; Group II – dams intoxicated with 60 fumonisins B (FB)/kg b.w. during gestation; Group III – dams intoxicated with 90 FB/kg b.w. during gestation. The data are presented as mean values ± standard error of the mean (n = 6), except for (f), where the line indicates the median, the box represents the interquartile range, and the whiskers show minimum and maximum value ranges. * – statistical significance at P-value < 0.05; ** – P-value < 0.01; *** – P-value < 0.001

Fig. 3.

Effects of prenatal fumonisins exposure on general bone innervation: A – representative photomicrographs showing protein gene product 9.5 (PGP9.5)-positive neuronal nets (arrowheads) in compact bone, trabecular bone and bone marrow; B – general bone innervation pattern quantified by morphology of the PGP9.5-positive neurons: i – spatial distribution of PGP9.5-positive neurons; ii – average cross-sectional area of PGP9.5-positive neuronal protrusions; iii – neuronal protrusion cross-sectional density of PGP9.5-positive neurons; iv – average length of neuronal trees in PGP9.5-positive neurons; v – mean number of branches in PGP9.5-positive neurons. Group I – control group; Group II – newborns from dams intoxicated with 60 fumonisins B (FB)/kg b.w. during gestation; Group III – newborns from dams intoxicated with 90 FB/kg b.w. during gestation. The data are presented as mean values ± standard error of the mean (n = 6); * – statistical significance at P-value < 0.05; ** – P-value < 0.01; *** – P-value < 0.001; scale bars – 20 μm
Effects of prenatal fumonisins exposure on general bone innervation: A – representative photomicrographs showing protein gene product 9.5 (PGP9.5)-positive neuronal nets (arrowheads) in compact bone, trabecular bone and bone marrow; B – general bone innervation pattern quantified by morphology of the PGP9.5-positive neurons: i – spatial distribution of PGP9.5-positive neurons; ii – average cross-sectional area of PGP9.5-positive neuronal protrusions; iii – neuronal protrusion cross-sectional density of PGP9.5-positive neurons; iv – average length of neuronal trees in PGP9.5-positive neurons; v – mean number of branches in PGP9.5-positive neurons. Group I – control group; Group II – newborns from dams intoxicated with 60 fumonisins B (FB)/kg b.w. during gestation; Group III – newborns from dams intoxicated with 90 FB/kg b.w. during gestation. The data are presented as mean values ± standard error of the mean (n = 6); * – statistical significance at P-value < 0.05; ** – P-value < 0.01; *** – P-value < 0.001; scale bars – 20 μm

Fig. 4.

Effects of prenatal fumonisin exposure on bone innervation: A – sympathetic and B and C – parasympathetic patterns quantified by morphology of the immunoreactive (IR) neurons. A – tyrosine hydroxylase (TH)-positive; B – choline acetyltransferase (ChAT)-positive; C – vasoactive intestinal peptide (VIP)-positive neurons: i – spatial distribution of IR neurons; ii – average cross-sectional area of IR neuronal protrusions; iii – neuronal protrusion cross-sectional density of IR neurons; iv – average length of neuronal trees in IR neurons; v – mean number of branches in IR neurons. Group I – control group; Group II – newborns from dams intoxicated with 60 fumonisins B (FB)/kg b.w. during gestation; Group III – newborns from dams intoxicated with 90 FB/kg b.w. during gestation. The data are presented as mean values ± standard error of the mean (n = 6); * – statistical significance at P-value < 0.05; ** – P-value < 0.01; *** – P-value < 0.001
Effects of prenatal fumonisin exposure on bone innervation: A – sympathetic and B and C – parasympathetic patterns quantified by morphology of the immunoreactive (IR) neurons. A – tyrosine hydroxylase (TH)-positive; B – choline acetyltransferase (ChAT)-positive; C – vasoactive intestinal peptide (VIP)-positive neurons: i – spatial distribution of IR neurons; ii – average cross-sectional area of IR neuronal protrusions; iii – neuronal protrusion cross-sectional density of IR neurons; iv – average length of neuronal trees in IR neurons; v – mean number of branches in IR neurons. Group I – control group; Group II – newborns from dams intoxicated with 60 fumonisins B (FB)/kg b.w. during gestation; Group III – newborns from dams intoxicated with 90 FB/kg b.w. during gestation. The data are presented as mean values ± standard error of the mean (n = 6); * – statistical significance at P-value < 0.05; ** – P-value < 0.01; *** – P-value < 0.001

Fig. 5.

Effects of prenatal fumonisins exposure on bone innervation: A and B – sensory and C – cocaine- and amphetamine-regulated transcript (CART)-positive patterns quantified by morphology of the immunoreactive (IR) neurons. A – SP-positive; B – GAL-positive; C – CART-positive neurons; i – spatial distribution of IR neurons; ii – average cross-sectional area of IR neuronal protrusions; iii – neuronal protrusion cross-sectional density of IR neurons; iv – average length of neuronal trees in IR neurons; v – mean number of branches in IR neurons. Group I – control group; Group II – newborns from dams intoxicated with 60 fumonisins B (FB)/kg b.w. during gestation; Group III – newborns from dams intoxicated with 90 FB/kg b.w. during gestation. The data are presented as mean values ± standard error of the mean (n = 6); * – statistical significance at P-value < 0.05; ** – P-value < 0.01; *** – P-value < 0.001
Effects of prenatal fumonisins exposure on bone innervation: A and B – sensory and C – cocaine- and amphetamine-regulated transcript (CART)-positive patterns quantified by morphology of the immunoreactive (IR) neurons. A – SP-positive; B – GAL-positive; C – CART-positive neurons; i – spatial distribution of IR neurons; ii – average cross-sectional area of IR neuronal protrusions; iii – neuronal protrusion cross-sectional density of IR neurons; iv – average length of neuronal trees in IR neurons; v – mean number of branches in IR neurons. Group I – control group; Group II – newborns from dams intoxicated with 60 fumonisins B (FB)/kg b.w. during gestation; Group III – newborns from dams intoxicated with 90 FB/kg b.w. during gestation. The data are presented as mean values ± standard error of the mean (n = 6); * – statistical significance at P-value < 0.05; ** – P-value < 0.01; *** – P-value < 0.001
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