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The effect of coffee husks used as pellet bedding material on the intestinal barrier, immune-related gene expression and microbiota composition in the broiler chicken caecum

Jakub Biesek

Jakub Biesek

Department of Animal Breeding and NutritionBydgoszcz, Poland
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Biesek, Jakub
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Aleksandra Dunisławska

Aleksandra Dunisławska

Department of Animal Biotechnology and Genetics, Faculty of Animal Breeding and Biology, Bydgoszcz University of Science and TechnologyBydgoszcz, Poland
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Dunisławska, Aleksandra
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Wojciech Kozdruń

Wojciech Kozdruń

Department of Poultry Diseases, National Veterinary Research InstitutePuławy, Poland
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Kozdruń, Wojciech
  
23. Sept. 2024
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Online veröffentlicht: 23. Sept. 2024
Seitenbereich: 443 - 449
Eingereicht: 28. März 2024
Akzeptiert: 09. Sept. 2024
DOI: https://doi.org/10.2478/jvetres-2024-0053
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© 2024 Jakub Biesek et al., published by Sciendo
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Fig. 1.

Relative analysis of expression of a panel of genes related to intestinal immune response and organism defence against pathogens in the caecal mucosa of broiler chickens bedded on litter with different proportions of coffee husks and wheat straw. CH10 – broilers with litter of 10% coffee husks and 90% wheat straw; CH25 – broilers with litter of 25% coffee husks and 75% wheat straw; CH50 – broilers with litter of 50% coffee husks and 50% wheat straw; * – P ≤ 0.05 (n = 8/group)
Relative analysis of expression of a panel of genes related to intestinal immune response and organism defence against pathogens in the caecal mucosa of broiler chickens bedded on litter with different proportions of coffee husks and wheat straw. CH10 – broilers with litter of 10% coffee husks and 90% wheat straw; CH25 – broilers with litter of 25% coffee husks and 75% wheat straw; CH50 – broilers with litter of 50% coffee husks and 50% wheat straw; * – P ≤ 0.05 (n = 8/group)

Fig. 2.

Relative abundance of indicator bacteria in caecal content of broiler chickens. C – broilers in the control group with litter of 100% wheat straw; CH10 – broilers with litter of 10% coffee husks and 90% wheat straw; CH25 – broilers with litter of 25% coffee husks and 75% wheat straw; CH50 – broilers with litter of 50% coffee husks and 50% wheat straw; * – P ≤ 0.05 (n = 8/group)
Relative abundance of indicator bacteria in caecal content of broiler chickens. C – broilers in the control group with litter of 100% wheat straw; CH10 – broilers with litter of 10% coffee husks and 90% wheat straw; CH25 – broilers with litter of 25% coffee husks and 75% wheat straw; CH50 – broilers with litter of 50% coffee husks and 50% wheat straw; * – P ≤ 0.05 (n = 8/group)

Primer sequences used in the RT-qPCR reaction

Gene Forward primer Reverse primer
ACTB CACAGATCATGTTTGAGACCTT CATCACAATACCAGTGGTACG
G6PDH CGGGAACCAAATGCACTTCGT GGCTGCCGTAGAGGTATGGGA
CLDN1 TCTTCATCATTGCAGGTCTGTC AACGGGTGTGAAAGGGTCAT
TJAP1 AGGAAGCGATGAATCCCTGTT TCACTCAGATGCCAGATCCAA
MUC6 TTCAACATTCAGTTCCGCCG TTGATGACACCGACACTCCT
AvBD1 AAACCATTGTCAGCCCTGTG TTCCTAGAGCCTGGGAGGAT
CATHL2 AGGAGAATGGGGTCATCAGG GGATCTTTCTCAGGAAGCGG
IL1B GGAGGTTTTTGAGCCCGTC TCGAAGATGTCGAAGGACTG
IL4 GCTCTCAGTGCCGCTGATG GGAAACCTCTCCCTGGATGTC
IL6 AGGACGAGATGTGCAAGAAGTTC TTGGGCAGGTTGAGGTTGTT
IL10 CATGCTGCTGGGCCTGAA CGTCTCCTTGATCTGCTTGATG
IL12B TTGCCGAAGAGCACCAGCCG CGGTGTGCTCCAGGTCTTGGG
IFNB ACCAGATCCAGCATTACATCCA CGCGTGCCTTGGTTTACG
IFNG ACACTGACAAGTCAAAGCCGC AGTCGTTCATCGGGAGCTTG

Primer sequences for the identification of bacteria

Bacteria Forward primer Reverse primer Reference
Universal bacteria ACTCCTACGGGAGGCAGCAGT GTATTACCGCGGCTGCTGGCAC (27)
Clostridium difficile TTGAGCGATTTACTTCGGTAAAGA TGTACTGGCTCACCTTTGATATTCA (23)
Faecalibacterium prausnitzii ACCATGAGAGCCGGGGGG GGTTACCTTGTTACGACTT (18)
Bifidobacterium spp. GCGTGCTTAACACATGCAAGTC CACCCGTTTCCAGGAGCTATT (23)
Lactobacillus spp. AGCAGTAGGGAATCTTCCA CACCGCTACACATGGAG (13)
Escherichia coli CATGCCGCGTGTATGAAGAA CGGGTAACGTCAATGAGCAAA (23)
Streptococcus spp. AGATGGACCTGCGTTGT GCTGCCTCCCGTAGGAGTCT (29)
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