<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec>
<h3>Introduction</h3>
<p>Outbreaks of fowl adenovirus (FAdV) infection in chicken flocks in Poland threaten birds’ health and lives and are rising in frequency. The risk of these infections in immunocompromised poultry flocks with developed clinical symptoms was analysed through virus detection in broiler chicks and correlation of cases with the birds’ immune strength.</p>
</sec>
<sec>
<h3>Material and Methods</h3>
<p>Samples were analysed from four broiler farms with chicks from the same hatchery in Silesia, Poland where feeding regimes were different. A normal diet was provided to birds on the control farm; a normal diet and probiotic, prebiotic, vitamin and microelement supplementation was supplied on another farm; a normal diet and antibiotics on the third; and a normal diet and both forms of supplementation were given on the fourth farm. Amplification of the virus DNA in a PCR with hexon gene L1 loop hypervariable region 1–4 primers determined the molecular characteristics of isolates of adenovirus strains obtained from necropsy tissue samples. The amplicon sequences were analysed, the pair-wise distances were determined, the maximum likelihood estimate for the gamma parameter for site rates was produced, Tajima’s D neutrality test was run and the relative synonymous codon usage and transition/transversion bias were calculated.</p>
</sec>
<sec>
<h3>Results</h3>
<p>Two species and two serotypes of fowl adenovirus – MW353018-FAdV-1/A-L-liver and MW353019-FAdV-5/B-I-intestine – were isolated in three-week-old broiler chicks on the control farm.</p>
</sec>
<sec>
<h3>Conclusion</h3>
<p>Supplementation of broiler chicken flocks with probiotics, prebiotics, vitamins and microelements may have a significant beneficial effect on immunity and can prevent virus infection. The studies provided new information on the molecular characteristics of adenovirus strains isolated from chicks with a low level of immunity.</p>
</sec>
</abstract>

Introduction
Outbreaks of fowl adenovirus (FAdV) infection in chicken flocks in Poland threaten birds’ health and lives and are rising in frequency. The risk of these infections in immunocompromised poultry flocks with developed clinical symptoms was analysed through virus detection in broiler chicks and correlation of cases with the birds’ immune strength.


Material and Methods
Samples were analysed from four broiler farms with chicks from the same hatchery in Silesia, Poland where feeding regimes were different. A normal diet was provided to birds on the control farm; a normal diet and probiotic, prebiotic, vitamin and microelement supplementation was supplied on another farm; a normal diet and antibiotics on the third; and a normal diet and both forms of supplementation were given on the fourth farm. Amplification of the virus DNA in a PCR with hexon gene L1 loop hypervariable region 1–4 primers determined the molecular characteristics of isolates of adenovirus strains obtained from necropsy tissue samples. The amplicon sequences were analysed, the pair-wise distances were determined, the maximum likelihood estimate for the gamma parameter for site rates was produced, Tajima’s D neutrality test was run and the relative synonymous codon usage and transition/transversion bias were calculated.


Results
Two species and two serotypes of fowl adenovirus – MW353018-FAdV-1/A-L-liver and MW353019-FAdV-5/B-I-intestine – were isolated in three-week-old broiler chicks on the control farm.


Conclusion
Supplementation of broiler chicken flocks with probiotics, prebiotics, vitamins and microelements may have a significant beneficial effect on immunity and can prevent virus infection. The studies provided new information on the molecular characteristics of adenovirus strains isolated from chicks with a low level of immunity.

Molecular characteristics of fowl adenovirus strains detected in broiler chickens on diets without immunostimulant supplements

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction
Outbreaks of fowl adenovirus (FAdV) infection in chicken flocks in Poland threaten birds’ health and lives and are rising in frequency. The risk...

Molecular characteristics of fowl adenovirus strains detected in broiler chickens on diets without immunostimulant supplements

Online veröffentlicht: 20. Mai 2024

Seitenbereich: -

Eingereicht: 01. Feb. 2024

Akzeptiert: 15. Mai 2024

DOI: https://doi.org/10.2478/jvetres-2024-0028

Schlüsselwörter
adenovirus infection, antibiotic, chickens, immunity, supplementation

© 2024 Jowita Samanta Niczyporuk et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Molecular characteristics of fowl adenovirus strains detected in broiler chickens on diets without immunostimulant supplements

Online veröffentlicht: 20. Mai 2024

Seitenbereich: -

Eingereicht: 01. Feb. 2024

Akzeptiert: 15. Mai 2024

DOI: https://doi.org/10.2478/jvetres-2024-0028

Schlüsselwörteradenovirus infection, antibiotic, chickens, immunity, supplementation

© 2024 Jowita Samanta Niczyporuk et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Schlüsselwörter
adenovirus infection, antibiotic, chickens, immunity, supplementation