The use of plant extracts and bacteriophages as an alternative therapy approach in combatting bacterial infections: the study of lytic phages and Stevia rebaudiana
Xymena Stachurska
Orcid-Profil, Małgorzata Mizielińska
Orcid-Profil, Magdalena Ordon
Orcid-Profil und Paweł Nawrotek
Orcid-Profil 
Online veröffentlicht: 19. Dez. 2023
Seitenbereich: 545 - 557
Eingereicht: 13. Mai 2023
Akzeptiert: 12. Okt. 2023
DOI: https://doi.org/10.2478/jvetres-2023-0059
Schlüsselwörter
© 2023 Xymena Stachurska et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Introduction
In the light of the problem of antibiotic resistance, the use of combined alternative therapies in combatting bacteria-related disorders has gained popularity. Bacteriophages are one element implemented in new combination therapy.
Material and Methods
The interactions were tested using a microdilution method, phage-extract co-incubation assay, static interaction (synography) and dynamic growth profile experiments in a bioreactor.
Results
The interactions of the tested factors in a static environment differed from those in a dynamic environment. Dynamic conditions altered the effect of the extracts in a concentration-dependent manner. How different the effect of the SRa extract was to that of the SRm extract on bacterial growth in a dynamic environment depended on the species of the phage and bacterial host. The greatest differences were observed for
Conclusion
Every extract type should be tested on a case-by-case basis and experiment outcomes should not be generalised before gathering data. Moreover, many varied experiments should be performed, especially when examining such multifactorial mixtures. The tested mixtures could potentially be used in multidrug-resistant bacterial infection treatments.