Uneingeschränkter Zugang

Utilisation of Actiphage in combination with IS900 qPCR as a diagnostic tool for rapid determination of paratuberculosis infection status in small ruminant herds

Monika Beinhauerova

Monika Beinhauerova

  • Department of Microbiology and Antimicrobial Resistance, Veterinary Research InstituteBrno, Czech Republic
  • Department of Experimental Biology, Faculty of Science, Masaryk UniversityBrno, Czech Republic
Suche nach diesem Autor auf
Sciendo|Google Scholar
Beinhauerova, Monika
 und 
Iva Slana

Iva Slana

Department of Microbiology and Antimicrobial Resistance, Veterinary Research InstituteBrno, Czech Republic
Suche nach diesem Autor auf
Sciendo|Google Scholar
Slana, Iva
  
20. Sept. 2023
Utilisation of Actiphage in combination with IS900 qPCR as a diagnostic tool for rapid determination of paratuberculosis infection status in small ruminant herds's Cover Image
Über diesen Artikel
Vorheriger Artikel
Nächster Artikel
Zitieren
COVER HERUNTERLADEN
Online veröffentlicht: 20. Sept. 2023
Seitenbereich: 347 - 352
Eingereicht: 20. Feb. 2023
Akzeptiert: 04. Juli 2023
DOI: https://doi.org/10.2478/jvetres-2023-0041
Schlüsselwörter
© 2023 Monika Beinhauerova et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction

Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of paratuberculosis, a chronic infectious intestinal disease occurring in domestic and wild ruminants. Early diagnosis of infected herds enabling timely adoption of control measures is tremendously important in view of the fact that the disease has a significant economic impact on farmers. The aim of this study was to evaluate the possibility of rapid detection of viable MAP on small ruminant farms based on environmental sample examination using a novel phage-based test named Actiphage.

Material and Methods

A total of 9 fresh and 28 frozen (8 or 11 years at -70°C) environmental samples originating from paratuberculosis-affected farms were analysed for the presence of MAP by four different diagnostic methods: Actiphage combined with real-time PCR targeting insertion sequence 900 (IS900 qPCR), conventional phage amplification assay, culture (frozen samples only), and direct ĪS900 qPCR.

Results

Viable MAP was detected in one fresh environmental sample using Actiphage–IS900 qPCR. None of the frozen samples tested positive using this diagnostic approach, which was consistent with the results of culture examination also providing information on viability.

Conclusion

This study describes other possible and innovative uses of phage-based methods in paratuberculosis control strategies. The Actiphage-qPCR was found to be less laborious than culture and provided results within six hours, suggesting that it may be a valuable tool for rapid initial determination of the infectious status of farmed animals based on environmental sample examination.
Sprache:
Englisch
Zeitrahmen der Veröffentlichung:
4 Hefte pro Jahr
Fachgebiete der Zeitschrift:
Biologie, Molekularbiologie, Mikrobiologie und Virologie, Biologie, andere, Medizin, Veterinärmedizin
Zeitschrift RSS Feed