Staining technique | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
Eosin- nigrosin | Sperm Stain | SpermBlue | Complex of eosin and gentian violet | Complex of eosin and gentian violet | Live/dead dual fluorescent staining method | Live/dead dual fluorescent staining method | Bengal rose stain | Bengal rose stain | Haematoxylin and eosin | |
Morphometric parameter | ||||||||||
Notes (reference) | ||||||||||
Season beginning, June ( |
Season end, August ( |
Large and small drone serum sampled from queenright ( |
Laying worker colonies ( |
Brazil, rainy season ( |
Brazil, dry season ( |
( |
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Acrosome length (μm) | 3.74a ± 0.61 | 3.59b ± 0.45 | - | 4.73 ± 0.32 | 4.66 ± 0.32 | - | - | - | - | 3.58 ± 1.21 |
Nucleus length (μm) | 4.25a ± 0.37 | 4.06b ± 0.23 | - | 4.78 ± 0.25 | 4.69 ± 0.25 | - | - | - | - | 4.44 ± 0.61 |
Head length (μm) | 8.08a ± 0.40 | 7.85b ± 0.29 | 7.71b ± 0.53 | 9.43 ± 0.38 | 9.35 ± 0.39 | - | - | 9.48 ± 0.10 | 9.24 ± 0.10 | 7.85 ± 0.65 |
Midpiece length (μm) | 30.63 ± 0.87 | - | - | - | - | - | - | - | - | - |
Flagellum length (μm) | 204.31 ± 10.12 | 226.16* ± 9.53 | 221.56* ± 11.29 | 264.07* ± 16.57 | 248.62* ± 16.38 | - | - | 253.47 ± 1.20 | 242.99 ± 4.00 | 222.96 ± 17.15 |
Total length (μm) | 242.59a ± 11.54 | 232.33a ± 11.01 | 226.56a ± 12.03 | 273.50 ± 16.58 | 257.97 ± 16.37 | 242.0 ± 0.65 | 241.4 ± 0.34 | 262.92 ± 1.20 | 252.24 ± 4.10 | 230.81 ± 17.22 |