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Vaccine-induced rabies in a red fox in Poland

Marcin Smreczak

Marcin Smreczak

Department of Virology, National Veterinary Research InstitutePuławy, Poland
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Smreczak, Marcin
, 
Anna Orłowska

Anna Orłowska

Department of Virology, National Veterinary Research InstitutePuławy, Poland
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Orłowska, Anna
, 
Thomas Müller

Thomas Müller

Institute for Molecular Virology and Cell Biology, Friedrich-Loeffler-Institute (FLI)Greifswald-Insel Riems, Germany
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Müller, Thomas
, 
Conrad M. Freuling

Conrad M. Freuling

Friedrich-Loeffler-Institute (FLI)Greifswald-Insel Riems, Germany
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Freuling, Conrad M.
, 
Magdalena Kawiak-Sadurska

Magdalena Kawiak-Sadurska

Department of Virology, National Veterinary Research InstitutePuławy, Poland
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Kawiak-Sadurska, Magdalena
 und 
Paweł Trębas

Paweł Trębas

Department of Virology, National Veterinary Research InstitutePuławy, Poland
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Trębas, Paweł
  
15. Nov. 2022
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Online veröffentlicht: 15. Nov. 2022
Seitenbereich: 473 - 477
Eingereicht: 03. Juni 2022
Akzeptiert: 03. Nov. 2022
DOI: https://doi.org/10.2478/jvetres-2022-0065
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© 2022 M. Smreczak et al. published by Sciendo
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Fig. 1

PCR-RFLP results using Dra I (A), Mbo II (B), Msp I (C) and Nla IV (D) restriction endonucleases. M – DNA molecular mass ruler; 1 – vaccine-induced rabies, fox; 2 – rabies vaccine strain Street Alabama Duferrin Bern; 3 – rabies virus street/field strain
PCR-RFLP results using Dra I (A), Mbo II (B), Msp I (C) and Nla IV (D) restriction endonucleases. M – DNA molecular mass ruler; 1 – vaccine-induced rabies, fox; 2 – rabies vaccine strain Street Alabama Duferrin Bern; 3 – rabies virus street/field strain

Fig. 2

Phylogenetic relationship of the vaccine-induced rabies virus isolated from a fox in Poland (indicated in the blue box) with reference rabies virus (RABV) isolates available in GenBank based on complete genomes. The phylogenetic tree was generated using the neighbour-joining method (Kimura2 parameter) in MEGA5 software. Bootstrap values (1,000 replicates) over 70% indicating significant support for the tree topology are shown next to the branches
Phylogenetic relationship of the vaccine-induced rabies virus isolated from a fox in Poland (indicated in the blue box) with reference rabies virus (RABV) isolates available in GenBank based on complete genomes. The phylogenetic tree was generated using the neighbour-joining method (Kimura2 parameter) in MEGA5 software. Bootstrap values (1,000 replicates) over 70% indicating significant support for the tree topology are shown next to the branches

Fig. 3

Section of canine teeth showing yellow fluorescent lines of tetracycline
Section of canine teeth showing yellow fluorescent lines of tetracycline

Distribution of viral RNA in particular predefined organs

Organ Threshold cycle Copies/Reaction
Brain 19.37 9.01e+005
Spinal cord 23.03 7.25e +004
Salivary glands 36.79 5.62
Tongue 26.62 6.14e+003
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