Postpartum dysgalactia syndrome (PDS) is a frequently observed health disorder of sows in modern pig farming (13, 18). The occurrence of PDS in sows can cause large financial losses and affect sow welfare (21). Although PDS directly affects the sows, it has a substantial influence on neonates’ vitality and health, which is linked with milk production disturbances. The aetiology of the syndrome is complex and not fully elucidated. The literature identified various risk factors for PDS occurrence, including bacteria of the genera
The sow’s serum is a source of the Ig present in the colostrum (24, 29) and colostrogenesis in the mammary glands begins before parturition at about 90 days of gestation; intending to find inferences from this, the study sought to determine the pattern of variation of serum Ig concentrations around the periparturient period in clinically healthy sows and sows suffering from PDS.
Parturition is linked to an inflammatory response as a normal physiological occurrence in sows and other females of domestic species such as mares and cows, and also in human females (6, 20, 22, 25, 28). However, the literature shows that PDS is associated with significant inflammatory overload compared to healthy sows (11, 22, 25, 28). The second objective of our study was to identify whether serum cortisol levels reflected the degree of stress and inflammation as well as sows’ welfare and whether differences between healthy and PDS-affected females occurred.
The clinical condition of the sows during the whole peripartum period was monitored by professional veterinary staff. An examination card was created for each sow for recording numerous clinical and production parameters as given in Table 1.
The clinical examination card of experimental sows
Sow no... | ….....Genetics………… | Parity……………………………………………… | Date of inclusion in examination…….. | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Date | Date | Date | Date | Date | Date | Date | Date | Date | |||
Rectal temperature (°C) | −14 | −7 | −3 | −1 | 0 | +1 | +3 | +7 | +14 | Treatment: | |
Duration of pregnancy | Duration of parturition | ||||||||||
Number litter of piglets in a | Number of live piglets/litter | ||||||||||
Number litter of stillbirths/ | Number of mummified piglets/litter | Number weaned piglets of | |||||||||
Appetite * Preserved 0 Decreased 1 (I, II, III) | |||||||||||
Milk production Normal N | PARTURITION | Date | Date | Date | Date | Date | |||||
Hypogalactia H Agalactia A | +1 | +3 | +5 | +7 | +14 | ||||||
PDS** Yes – 1 | PDS severity*** | Date | Date | Date | Date | Date | |||||
No – 0 | (I, II, III) | +1 | +3 | +5 | +7 | +14 | |||||
Other disorders (date, type) |
* Appetite scores were – 0 – preserved (eats more than 80% of daily portion); I – decreased: (eats 50–80% of daily portion); II – decreased (eats less than 50% of daily portion); III – lack of appetite (does not eat)
** If at least 1 teat was inflamed, PDS was defined as 1, and cases with no lesions were defined as 0
*** PDS severity: I – mild (1–2 teats); II – moderate (3–6 teats); III – severe (≥ 7 teats)
Based on the clinical course of the peripartum period, the sows from both herds were divided into two groups: H (n = 58), a group of clinically healthy sows with a physiological peripartum period and PDS (n = 45) a group of sows with milk production disorders (hypo-or agalactia) and mastitis. Both groups H and PDS comprised only multiparous sows (from second to sixth parity). For group H and group PDS, gestation length ranged from 112 to 119 days and from 113 to 120 days, respectively. In healthy sows, farrowing lasted from 2 to 5.5 h, whereas in PDS-affected sows, farrowing lasted from 2.9 to 10.5 h. The number of piglets born per litter of sows from group H and group PDS was between 9 and 24 piglets and 9 and 25 piglets, respectively. Sows with complicated parturitions requiring farrowing assistance, inflammations not connected with mammary glands (abscesses or hoof infections), or lameness were excluded from the study.
The patterns of variation of the mean concentrations of IgG, IgM and IgA from 28 days before parturition to 28 days postpartum in the healthy and PDS groups are presented in Figs 1–3. In healthy sows, mean serum concentrations of IgG 28 and 14 days before parturition were above 36 mg/mL and then gradually decreased until the first day postpartum (23 mg/mL) and remained relatively stable until 7 days after parturition.
Changes in serum immunoglobulin G (IgG) concentrations (mean ± SD) around the peripartum period in sows from individual experimental groups. PDS – postpartum dysgalactia syndrome; * – statistically significant differences compared to day −28 and day −14 in the healthy group; (P < 0.05); ** – statistically significant differences compared to day 28 and day 14 in the healthy group; (P < 0.05)
Changes in serum immunoglobulin M (IgM) concentrations (mean ± SD) around the peripartum period in sows from individual experimental groups. PDS – postpartum dysgalactia syndrome
Changes in serum immunoglobulin A (IgA) concentrations (mean ± SD) around the peripartum period in sows from individual experimental groups. PDS – postpartum dysgalactia syndrome
Starting from day 14 of lactation to the end of the study, the content of IgG increased almost 1.5-fold (from 24 mg/mL to 34.6 mg/mL). Moreover, on days 3 and 7 after parturition, the amount of IgG was significantly lower than on days 28 and 14 before parturition (P < 0.05). In the PDS group, the pattern of IgG concentrations was highly similar to those observed in healthy sows. However, no statistically significant difference was observed at any time point (P > 0.05).
Regarding IgM, its serum concentrations were relatively stable throughout the entire experiment and did not present any statistically significant variation over time nor between group H and group PDS (P > 0.05). Similarly to IgM, the IgA content did not reveal any statistically significant fluctuation at any time point either in analysed groups or between the groups (P > 0.05).
Regarding cortisol, its mean content remained at a highly similar level throughout the entire experiment (P > 0.05), with a large inter-sow variation in both healthy and PDS-affected sows (Fig. 4). The mean cortisol level ranged between 16.6 ng/mL and 24.9 ng/mL in group PDS and between 18.3 ng/mL to 24.2 ng/mL in group H. Moreover, farrowing itself also did not induce significant changes either in the mean or individual cortisol concentrations.
Changes in serum cortisol concentrations (mean ± SD) around the peripartum in sows from individual experimental groups. PDS – postpartum dysgalactia syndrome
The present study was undertaken to analyse the pattern of changes in serum IgG, IgM and IgA concentrations in clinically healthy sows and sows with lactation impairment. To the authors’ knowledge, this is the first report of parturition-related variations of immunoglobulin in the serum of sows with postpartum dysgalactia syndrome.
Immunoglobulins and other immunomodulatory factors are integral parts of the colostrum and the most important constituents conferring immune competence to newborns (24). It is worth highlighting that colostral immunoglobulins are non-selectively transported from sow serum into the mammary gland (1). In sows, all colostrum IgG and almost 80% and 40% of colostrum IgM and IgA, respectively, are derived from serum. Therefore, an adequate content of Ig, mostly IgG, in sow serum is of great importance to newborns. Several previous studies identified multiple factors which may affect Ig content in colostrum,
In the current study, the IgA and IgM content in the serum showed highly similar profiles in PDS-affected sows and healthy ones. The evaluated groups also presented comparable IgG concentrations. However, in group H, a significantly higher concentration of IgG was observed on 28 and 14 days ante partum than on days 3 and 7 post partum. These observations are partially in line with the results of Devillers
Cortisol has been the object of previous studies concerning its utility as a marker of various stress conditions and inflammation, also those related to PDS (10). In swine, cortisol is acknowledged as the primary glucocorticoid hormone; its increase is related to hypothalamic–pituitary–adrenal axis activation by stress stimuli such as inflammatory processes (19). In the present study, serum cortisol concentration showed similar kinetics in sows from both analysed groups during the entire follow-up period. In contrast, a previous study on sows (clinically healthy and PDS-affected) revealed significantly higher cortisol concentration in the serum and saliva of PDS-affected sows than in healthy ones (10). The discrepancies in these findings may result from the different methods used in the previous study to evaluate cortisol concentration in the serum. Moreover, the follow-up period and time point of each sampling in the present study were not fully consistent with those of the experimental design of Kaiser
In summary, the present results demonstrated that lactation impairment in PDS did not influence immunoglobulin or cortisol concentrations in sow serum. Future studies are desirable to evaluate immunoglobulin content in colostrum and serum of piglets born to sows suffering from PDS.