Login
Registrieren
Passwort zurücksetzen
Veröffentlichen & Verteilen
Verlagslösungen
Vertriebslösungen
Themen
Allgemein
Altertumswissenschaften
Architektur und Design
Bibliotheks- und Informationswissenschaft, Buchwissenschaft
Biologie
Chemie
Geowissenschaften
Geschichte
Industrielle Chemie
Informatik
Jüdische Studien
Kulturwissenschaften
Kunst
Linguistik und Semiotik
Literaturwissenschaft
Materialwissenschaft
Mathematik
Medizin
Musik
Pharmazie
Philosophie
Physik
Rechtswissenschaften
Sozialwissenschaften
Sport und Freizeit
Technik
Theologie und Religion
Wirtschaftswissenschaften
Veröffentlichungen
Zeitschriften
Bücher
Konferenzberichte
Verlage
Blog
Kontakt
Suche
EUR
USD
GBP
Deutsch
English
Deutsch
Polski
Español
Français
Italiano
Warenkorb
Home
Zeitschriften
Journal of Veterinary Research
Band 65 (2021): Heft 4 (December 2021)
Uneingeschränkter Zugang
The protective action of piperlongumine against mycobacterial pulmonary tuberculosis in its mitigation of inflammation and macrophage infiltration in male BALB/c mice
Nihong Lu
Nihong Lu
,
Yongrui Yang
Yongrui Yang
,
Xiaofei Li
Xiaofei Li
,
Jie Li
Jie Li
,
Jie Cheng
Jie Cheng
,
Zhengxuan Lv
Zhengxuan Lv
und
Yingrong Du
Yingrong Du
| 20. Nov. 2021
Journal of Veterinary Research
Band 65 (2021): Heft 4 (December 2021)
Über diesen Artikel
Vorheriger Artikel
Nächster Artikel
Zusammenfassung
Artikel
Figuren und Tabellen
Referenzen
Autoren
Artikel in dieser Ausgabe
Vorschau
PDF
Zitieren
Teilen
Article Category:
Review article
Online veröffentlicht:
20. Nov. 2021
Seitenbereich:
431 - 440
Eingereicht:
02. Apr. 2021
Akzeptiert:
26. Okt. 2021
DOI:
https://doi.org/10.2478/jvetres-2021-0061
Schlüsselwörter
tuberculosis
,
piperlongumine
,
macrophages
,
inflammation
,
Mincle
© 2021 N. Lu et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Fig. 1
Effect of piperlongumine (PL) on cell viability in MH-S (6 × 105) cells cultured for 48 h with and without PL (2.5–45 μg/mL). Dimethyl sulphoxide (DMSO) was used as internal reference for comparison. Data are expressed as mean ± SD and show no significant differences between DMSO and PL treated cells
Fig. 2a
In vitro effects of piperlongumine (PL) on the inhibition production of TNF-α and IL-6 in trehalose 6,6-dimycolate (TDM)-activated MH-S cells. Data are expressed as mean ± SD of three experiments. DMSO – dimethyl sulphoxide; P1 –PL at 5 μg/mL; P2 – PL at 15 μg/mL; P3 – PL at 25 μg/mL; DEX – dexamethasone; ns – non-significant, P > 0.05; * P < 0.05; ** P < 0.01; *** P < 0.001 vs TDM-treated group
Fig. 2b
In vitro effects of piperlongumine (PL) on the inhibition of production of C-C motif chemokine ligand 2 (CCL-2), C-C motif chemokine ligand 10 (CXCL-10), C-C motif chemokine ligand 5 (CCL-5) and keratinocyte-derived chemokine (KC) in trehalose 6,6-dimycolate (TDM)-activated MH-S cells. Data are expressed as mean ± SD of three experiments. DMSO – dimethyl sulphoxide; P1 – PL 5 μg/mL; P2 – PL 15 μg/mL; P3 – PL 25 μg/mL; DEX – dexamethasone; ns – non-significant, P > 0.05; * P < 0.05; ** P < 0.01; *** P < 0.001 vs TDM-treated group
Fig. 3
Enhancing effect of piperlongumine (PL) on the clearance of mycobacterium from alveolar macrophages. Data are expressed as mean ± SD of three experiments. TDM – trehalose-6,6-dimycolate; DMSO – dimethyl sulphoxide; P1 – PL at 5 μg/mL; P2 – PL at 15 μg/mL; P3 – PL at 25 μg/mL; DEX – dexamethasone; ns – non-significant, P > 0.05; ** P < 0.01; *** P < 0.001 vs M. tuberculosis H37Rv strain infection group
Fig. 5
Diminution by piperlongumine of trehalose 6,6-dimycolate (TDM)-stimulated pulmonary granulomatous inflammation in BALB/c mice. Data are expressed as mean ± SD of three experiments. A) Haematoxylin and eosin-stained granulomatous response in BALB/c mice after 4–7 days of piperlongumine (PL) treatment after TDM challenge. B) Inflammatory intensity level in lungs removed from TDM-challenged mice on days 4 and 7. P1 – PL at 50 mg/mL; P2 – PL at 100 mg/mL; P3 – PL at 150 mg/mL; DEX – dexamethasone; ns – non-significant, P > 0.05; * P < 0.05; ** P < 0.01; *** P <0.001 vs TDM-treated group
Fig. 6
Effect of piperlongumine on trehalose 6,6-dimycolate (TDM)-stimulated pulmonary granulomatous inflammation in BALB/c mice. Data are expressed as mean ± SD of three experiments. Graphs show production of tumour necrosis factor alpha (TNF-α), interleukin 6 (IL-6), interleukin 10 (IL-10), keratinocyte-derived cytokine (KC), C-C motif chemokine ligand 5 (CCL-5), C-C motif chemokine ligand 10 (CXCL-10) and C-C motif chemokine ligand 2 (CCL-2) in pulmonary homogenates after 4 and 7 days of TDM stimulation with or without piperlongumine (PL) treatment. P1 – PL at 50 mg/mL; P2 – PL at 100 mg/mL; P3 – PL at 150 mg/mL; DEX – dexamethasone; ns – non-significant, P > 0.05; * P < 0.05; ** P < 0.01; *** P < 0.001 vs TDM-treated group
Fig. 7
Inhibition by piperlongumine (PL) of leukocyte infiltration in trehalose 6,6-dimycolate (TDM)-stimulated pulmonary granulomatous inflammation in BALB/c mice. Data are expressed as mean ± SD of three experiments. A) CD11b+ Ly6G−macrophages. B) CD11b+ lymphocyte antigen 6 complex locus G6D (Ly6G+)–neutrophils. C) chemokine receptor type 3 (CCR3)+ CD16/32−eosinophils. Bar charts represent quantitative measurement of positively stained cells. P1 – PL at 50 mg/mL; P2 – PL at 100 mg/mL; P3 –PL at 150 mg/mL; DEX – dexamethasone; ns – non-significant, P > 0.05; * P < 0.05; ** P < 0.01; *** P < 0.001 vs TDM-treated group
Vorschau