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Comparison of chicken immune responses after inoculation with H5 avian influenza virus-like particles produced by insect cells or pupae

Dean Huang

Dean Huang

School of Veterinary Medicine, National Taiwan UniversityTaipei, Taiwan
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Huang, Dean
, 
Yu-Chan Chao

Yu-Chan Chao

Academia SinicaTaipei, Taiwan
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Chao, Yu-Chan
, 
Zhengbing Lv

Zhengbing Lv

College of Life Sciences and Medicine, Zhejiang Sci-Tech UniversityHangzhou, China
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Lv, Zhengbing
, 
Jia-Tsrong Jan

Jia-Tsrong Jan

Academia SinicaTaipei, Taiwan
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Jan, Jia-Tsrong
, 
Yu-Chih Yang

Yu-Chih Yang

Academia SinicaTaipei, Taiwan
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Yang, Yu-Chih
, 
Pei-Wen Hsiao

Pei-Wen Hsiao

Academia SinicaTaipei, Taiwan
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Hsiao, Pei-Wen
, 
Chia-Ying Wu

Chia-Ying Wu

Academia SinicaTaipei, Taiwan
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Wu, Chia-Ying
, 
Chiu-Hsun Liao

Chiu-Hsun Liao

Miaoli District Agricultural Research and Extension Station, Council of AgricultureMiaoli, Taiwan
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Liao, Chiu-Hsun
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Tzu-Hsien Wu

Tzu-Hsien Wu

Miaoli District Agricultural Research and Extension Station, Council of AgricultureMiaoli, Taiwan
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Wu, Tzu-Hsien
 und 
Lih-Chiann Wang

Lih-Chiann Wang

School of Veterinary Medicine, National Taiwan UniversityTaipei, Taiwan
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Wang, Lih-Chiann
  
22. Mai 2021
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Online veröffentlicht: 22. Mai 2021
Seitenbereich: 139 - 145
Eingereicht: 25. Okt. 2020
Akzeptiert: 26. Apr. 2021
DOI: https://doi.org/10.2478/jvetres-2021-0026
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© 2021 D. Huang et al. published by Sciendo
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Fig. 1

Sf21 VLP and silkworm pupa VLP characterisation. A – Detection of HA protein and M1 protein in VLPs using Western blot; M – molecular weight markers; 1 – Sf21 VLP; 2 – silkworm pupa VLP. B – Detection of Sf21 VLP (up) and silkworm pupa VLP (down) under a transmission electron microscope; scale bar = 50 nm. C – Haemagglutination activity of the purified Sf21 VLP and silkworm pupa VLP
Sf21 VLP and silkworm pupa VLP characterisation. A – Detection of HA protein and M1 protein in VLPs using Western blot; M – molecular weight markers; 1 – Sf21 VLP; 2 – silkworm pupa VLP. B – Detection of Sf21 VLP (up) and silkworm pupa VLP (down) under a transmission electron microscope; scale bar = 50 nm. C – Haemagglutination activity of the purified Sf21 VLP and silkworm pupa VLP

Fig. 2

Immunogenicity of Sf21 VLP and silkworm pupa VLP. A – Serum HI titres against the corresponding VLP (left) or A/Anhui/1/2005 (H5N1, clade 2.3.4) (right). Error bars are mean ± SEM. ns – not significant. B – ELISA IgY titres on day 46 post-immunisation using the corresponding VLP (left) or A/ Anhui/1/2005 (H5N1, clade 2.3.4) (right) as antigens. Lines and boxes represent the upper extreme, 25th, 50th, and 75th percentiles, and the lower extreme. * – P < 0.05; ** – P < 0.01; *** – P < 0.001
Immunogenicity of Sf21 VLP and silkworm pupa VLP. A – Serum HI titres against the corresponding VLP (left) or A/Anhui/1/2005 (H5N1, clade 2.3.4) (right). Error bars are mean ± SEM. ns – not significant. B – ELISA IgY titres on day 46 post-immunisation using the corresponding VLP (left) or A/ Anhui/1/2005 (H5N1, clade 2.3.4) (right) as antigens. Lines and boxes represent the upper extreme, 25th, 50th, and 75th percentiles, and the lower extreme. * – P < 0.05; ** – P < 0.01; *** – P < 0.001

Fig. 3

Cell-mediated immune response induced by Sf21 VLP and silkworm pupa VLP in chickens. A – Splenic IFN-γ mRNA amplification in real-time PCR. B – IL-4 mRNA amplification in real-time PCR. C – Splenic CD4+/CD8+ ratios found using flow cytometry. Error bars are mean ± SEM. ns – not significant. * – P < 0.05; ** – P < 0.01; *** – P < 0.001
Cell-mediated immune response induced by Sf21 VLP and silkworm pupa VLP in chickens. A – Splenic IFN-γ mRNA amplification in real-time PCR. B – IL-4 mRNA amplification in real-time PCR. C – Splenic CD4+/CD8+ ratios found using flow cytometry. Error bars are mean ± SEM. ns – not significant. * – P < 0.05; ** – P < 0.01; *** – P < 0.001
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