<abstract xmlns="http://www.w3.org/1999/xhtml"><sec id="j_jvetres-2020-0065_s_005_w2aab3b7c13b1b6b1aab1c14b1Aa"><h3>Introduction</h3><p>Due to the widespread occurrence of acrylamide in the environment, its likely carcinogen status, and the suitability of the pig model as a human analogue, the authors decided to evaluate the impact of high and low doses of this compound on the processes of erythropoiesis in swine bone marrow.</p></sec><sec id="j_jvetres-2020-0065_s_006_w2aab3b7c13b1b6b1aab1c14b2Aa"><h3>Material and Methods</h3><p>The experiment was carried out on Danish Landrace pigs at the age of eight weeks and body weight about 20 kg. The animals were divided into three equal groups consisting of five pigs in each. Control animals received empty gelatin capsules (placebos). Animals from the first experimental group received a low dose of acrylamide of 0.5 μg/kg b.w./day (> 99% purity; Sigma-Aldrich, Poland), and animals from the second experimental group received a dose 10 times higher. Placebos and acrylamide capsules were administered with feed every morning for 28 days. After anaesthetisation of the animals, bone marrow from the femur was collected into tubes without an anticoagulant on days 0 and 28. After drying and staining, bone marrow smears were subjected to detailed cytological evaluation using a light microscope.</p></sec><sec id="j_jvetres-2020-0065_s_007_w2aab3b7c13b1b6b1aab1c14b3Aa"><h3>Results</h3><p>This study showed that high and low doses of acrylamide affected the process of porcine erythropoiesis. The cytotoxic effect of acrylamide on this process was demonstrated in a change of the polychromatic erythroblasts/normochromatic erythroblasts ratio.</p></sec><sec id="j_jvetres-2020-0065_s_008_w2aab3b7c13b1b6b1aab1c14b4Aa"><h3>Conclusion</h3><p>Both doses of acrylamide caused a decrease in the number of ortho- and polychromatic erythroblasts.</p></sec></abstract>

IntroductionDue to the widespread occurrence of acrylamide in the environment, its likely carcinogen status, and the suitability of the pig model as a human analogue, the authors decided to evaluate the impact of high and low doses of this compound on the processes of erythropoiesis in swine bone marrow.Material and MethodsThe experiment was carried out on Danish Landrace pigs at the age of eight weeks and body weight about 20 kg. The animals were divided into three equal groups consisting of five pigs in each. Control animals received empty gelatin capsules (placebos). Animals from the first experimental group received a low dose of acrylamide of 0.5 μg/kg b.w./day (> 99% purity; Sigma-Aldrich, Poland), and animals from the second experimental group received a dose 10 times higher. Placebos and acrylamide capsules were administered with feed every morning for 28 days. After anaesthetisation of the animals, bone marrow from the femur was collected into tubes without an anticoagulant on days 0 and 28. After drying and staining, bone marrow smears were subjected to detailed cytological evaluation using a light microscope.ResultsThis study showed that high and low doses of acrylamide affected the process of porcine erythropoiesis. The cytotoxic effect of acrylamide on this process was demonstrated in a change of the polychromatic erythroblasts/normochromatic erythroblasts ratio.ConclusionBoth doses of acrylamide caused a decrease in the number of ortho- and polychromatic erythroblasts.

The influence of high and low doses of acrylamide on porcine erythropoiesis

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"The influence of high and low doses of acrylamide on porcine erythropoiesis"}

IntroductionDue to the widespread occurrence of acrylamide in the environment, its likely carcinogen status, and the suitability of the pig model as a human...

The influence of high and low doses of acrylamide on porcine erythropoiesis

Online veröffentlicht: 15. Okt. 2020

Seitenbereich: 609 - 614

Eingereicht: 26. März 2020

Akzeptiert: 30. Sept. 2020

DOI: https://doi.org/10.2478/jvetres-2020-0065

Schlüsselwörter
swine, acrylamide, bone marrow, polychromatic erythrocytes

© 2020 A. Snarska et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The influence of high and low doses of acrylamide on porcine erythropoiesis

Online veröffentlicht: 15. Okt. 2020

Seitenbereich: 609 - 614

Eingereicht: 26. März 2020

Akzeptiert: 30. Sept. 2020

DOI: https://doi.org/10.2478/jvetres-2020-0065

Schlüsselwörterswine, acrylamide, bone marrow, polychromatic erythrocytes

© 2020 A. Snarska et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Schlüsselwörter
swine, acrylamide, bone marrow, polychromatic erythrocytes