African swine fever (ASF) is a pressing economic problem in a number of Eastern European countries. It has also depleted the Chinese sow population by 50%. Managing the disease relies on culling infected pigs or hunting wild boars as sanitary zone creation. The constraints on the development of an efficient vaccine are mainly the virus’ mechanisms of host immune response evasion. The study aimed to adapt a field ASFV strain to established cell lines and to construct recombinant African swine fever virus (ASFV) strain.
The host immune response modulation genes
The reconstituted virus showed similar kinetics of replication in comparison to the parent virus isolate.
Taking into account the usefulness of the developed CRISPR/Cas9 system it has been shown that modification of the