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Seroprevalence, clinical, and pathological characteristics of canine leishmaniasis in a central region of Colombia

Yenny Picón
Yenny Picón
, 
Geiner Almario
Geiner Almario
, 
Victoria Rodríguez
Victoria Rodríguez
 und 
Noel Verjan Garcia
Noel Verjan Garcia
   | 14. Feb. 2020
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Online veröffentlicht: 14. Feb. 2020
Seitenbereich: 85 - 94
Eingereicht: 28. März 2019
Akzeptiert: 03. Feb. 2020
DOI: https://doi.org/10.2478/jvetres-2020-0011
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© 2020 Y. Picón et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Macroscopic lesions in dogs with leishmaniasis in a central region of Colombia. A, B – skin lesions (exfoliative and ulcerative dermatitis) on the head, neck, and ears (Fig. B lower panel is a magnification of the delineated square in A). C, D – splenomegaly with nodules protruding on the irregular surface of the organ (Fig. D is a magnification of the delineated square in fig. C). E, F upper panel – ulcerated nodule in the lung (Fig. F upper panel is a magnification of the delineated square in Fig. E). F lower panel – a non-ulcerated nodule protruding from the surface of the lung of a different dog
Macroscopic lesions in dogs with leishmaniasis in a central region of Colombia. A, B – skin lesions (exfoliative and ulcerative dermatitis) on the head, neck, and ears (Fig. B lower panel is a magnification of the delineated square in A). C, D – splenomegaly with nodules protruding on the irregular surface of the organ (Fig. D is a magnification of the delineated square in fig. C). E, F upper panel – ulcerated nodule in the lung (Fig. F upper panel is a magnification of the delineated square in Fig. E). F lower panel – a non-ulcerated nodule protruding from the surface of the lung of a different dog

Fig. 2

Microscopic lesions in dogs with leishmaniasis in a central region of Colombia. A, B – auricular dermatitis associated with intracellular amastigotes present within macrophages (arrows). C, D – lymphadenitis showing abundant promastigotes parasites (arrows) within phagocytic mononuclear cells. E – spleen with severe depletion of lymphocytes (white pulp) and apparent hyperplasia of red pulp. F – lung with moderate to severe interstitial pneumonia. G – kidney showing interstitial nephritis with tubular degeneration. H – small intestine showing lymphoplasmacytic infiltrate
Microscopic lesions in dogs with leishmaniasis in a central region of Colombia. A, B – auricular dermatitis associated with intracellular amastigotes present within macrophages (arrows). C, D – lymphadenitis showing abundant promastigotes parasites (arrows) within phagocytic mononuclear cells. E – spleen with severe depletion of lymphocytes (white pulp) and apparent hyperplasia of red pulp. F – lung with moderate to severe interstitial pneumonia. G – kidney showing interstitial nephritis with tubular degeneration. H – small intestine showing lymphoplasmacytic infiltrate

Fig. 3

PCR Amplification of a 314 bp DNA fragment of a Leishmania RNA gene. Upper panel – M:100 bp ladder. Lane 1 – negative control (without template DNA). Lane 2 – positive control of L. amazonensis. Lane 3 – spleen. Lane 4 – skin. Lanes 5, 6 – lymph nodes. Lane 7 – positive control of L. infantum. Lane 8 – positive control of L. braziliensis. Lower panel – M: 100 bp ladder. Lane 1 – positive control of L. amazonensis. Lanes 2–5 – spleen samples from three serological positive dogs
PCR Amplification of a 314 bp DNA fragment of a Leishmania RNA gene. Upper panel – M:100 bp ladder. Lane 1 – negative control (without template DNA). Lane 2 – positive control of L. amazonensis. Lane 3 – spleen. Lane 4 – skin. Lanes 5, 6 – lymph nodes. Lane 7 – positive control of L. infantum. Lane 8 – positive control of L. braziliensis. Lower panel – M: 100 bp ladder. Lane 1 – positive control of L. amazonensis. Lanes 2–5 – spleen samples from three serological positive dogs

Epidemiological variables associated with leishmaniasis in dogs from a central region of Colombia (July 2016-July 2017)

Variables Tolima % Huila % Total % OR P < 0.05
Characteristics of housing
Bamboo 1 33.3 0 0.0 1 9.1
Brick 1 33.3 4 50.0 5 45.5
Wood 0 0.0 3 37.5 3 27.3
Gaudua 1 33.3 1 12.5 2 18.2 10.5 0.398
Sick people 0 0.0 2 25.0 2 18.2 7 0.221
Garbage 1 33.3 4 50.0 5 45.5 7 0.221
Reservoirs 3 100.0 8 100.0 11 100.0 8.93 0.003
House plants 3 100.0 7 87.5 10 90.9 7 0.221
Stream or pipes 2 66.7 7 87.5 9 81.8 2.91 0.713
Pests 2 66.7 7 87.5 9 81.8 8.25 0.004
Characteristics of canine
Male 3 100.0 7 87.5 10 90.9 7.35 0.007
Female 0 0.0 1 12.5 1 9.1
Age 6.3 3–11 3.6 2–5 4.4 2–11 14 0.173
Cachexia 2.917 0.713
Normal
Trips 1 33.3 4 50.0 5 45.5 5.125 0.024
Symptomatology
Anamnesis 3 100 7 87.5 10 90.9 4.95 0.421
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