Elaboration of triplex PCR for detection of selected viral infections in waterfowl
Artikel-Kategorie: Short communication
Online veröffentlicht: 19. Dez. 2019
Seitenbereich: 503 - 505
Eingereicht: 17. Juli 2019
Akzeptiert: 22. Nov. 2019
DOI: https://doi.org/10.2478/jvetres-2019-0074
Schlüsselwörter
© 2019 W. Kozdruń et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Introduction
Viral infections are the greatest threat to waterfowl and cause significant economic losses. Diagnosis and differentiation of three goose viruses is difficult in the field and often requires laboratory confirmation. Therefore, the aim of the study was to develop a triplex PCR and optimise its parameters for simultaneous detection of DNA of goose parvovirus (GPV), goose polyomavirus (GHPV), and goose circovirus (GoCV).
Material and Methods
The DNA of viruses isolated from field cases from the National Veterinary Research Institute’s own collection was used for the study. The primer attachment temperature, the number of reaction cycles, and the
Results
Based on the obtained results, triplex PCR parameters were optimised for simultaneous detection of DNA of GPV, GHPV, and GoCV in one sample. The following PCR products of the expected size were obtained: GPV DNA of 806 bp, GoCV DNA of 571 bp, and GHPV DNA of 180 bp.
Conclusion
The developed triplex PCR method proved to be useful for simultaneous detection of infections with three waterfowl viruses and will be used in relevant laboratory diagnostics.