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Early detection of active glomerular lesions in dogs and cats using podocin

Barbara Szczepankiewicz

Barbara Szczepankiewicz

Department of Internal Medicine and Clinic of Diseases of Horses, Dogs and Cats, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life SciencesWrocław, Poland
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Szczepankiewicz, Barbara
, 
Urszula Pasławska

Urszula Pasławska

  • Department of Internal Medicine and Clinic of Diseases of Horses, Dogs and Cats, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life SciencesWrocław, Poland
  • Institute of Veterinary Medicine, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University ToruńToruń, Poland
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Pasławska, Urszula
, 
Marcin Nowak

Marcin Nowak

Department of Pathology, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life SciencesWrocław, Poland
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Nowak, Marcin
, 
Remigiusz Bąchor

Remigiusz Bąchor

Department of Chemistry, Wrocław UniversityWrocław, Poland
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Bąchor, Remigiusz
, 
Agnieszka Czyżewska-Buczyńska

Agnieszka Czyżewska-Buczyńska

Regional Specialist Hospital in Wrocław, Research and Development UnitWrocław, Poland
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Czyżewska-Buczyńska, Agnieszka
, 
Robert Pasławski

Robert Pasławski

Institute of Veterinary Medicine, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University ToruńToruń, Poland
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Pasławski, Robert
 und 
Zbigniew Szewczuk

Zbigniew Szewczuk

Department of Chemistry, Wrocław UniversityWrocław, Poland
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Szewczuk, Zbigniew
  
19. Dez. 2019
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Artikel-Kategorie: Review Article
Online veröffentlicht: 19. Dez. 2019
Seitenbereich: 573 - 577
Eingereicht: 29. Juli 2019
Akzeptiert: 05. Nov. 2019
DOI: https://doi.org/10.2478/jvetres-2019-0062
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© 2019 B. Szczepankiewicz et al. published by Sciendo
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Fig. 1

A histopathological assessment of the kidney of a dog without blood azotaemia, suffering from cardiac insufficiency due to severe stage Da mitral endocardiosis according to the ACVIM classification (9) a – H-E stained section. Interstitial, lymphocytic inflammatory infiltration (black arrow) and glomerular hyalinosis and atrophy (white arrow) are visible; b – the immunohistochemical reaction in the kidney section of the same dog, using polyclonal rabbit anti-nephrin antibody (cat no. abx019258, Abexxa, UK, 1:200 dilution). A weak reaction indicates podocyte damage; c – a weak immunohistochemical reaction indicating podocyte damage, using polyclonal rabbit anti-podocin antibody (cat no. abx019257, Abexxa, 1:400 dilution). The sections were washed with Dako Wash Buffer (10×) (product code S3006, Agilent, USA). The Peroxidase/DAB+ Rabbit/Mouse Dako Real EnVision Detection System was used to visualise the immunohistochemical reaction (product code K8006, Agilent). The microphotographs of the studied sections come from our own research and were subjected to computer-aided image analysis using cellSens software (Olympus Soft Imaging Solution GmbH, Germany) coupled with an optical Olympus BX53 microscope equipped with a ColorView IIIu digital camera (Olympus, Japan)
A histopathological assessment of the kidney of a dog without blood azotaemia, suffering from cardiac insufficiency due to severe stage Da mitral endocardiosis according to the ACVIM classification (9) a – H-E stained section. Interstitial, lymphocytic inflammatory infiltration (black arrow) and glomerular hyalinosis and atrophy (white arrow) are visible; b – the immunohistochemical reaction in the kidney section of the same dog, using polyclonal rabbit anti-nephrin antibody (cat no. abx019258, Abexxa, UK, 1:200 dilution). A weak reaction indicates podocyte damage; c – a weak immunohistochemical reaction indicating podocyte damage, using polyclonal rabbit anti-podocin antibody (cat no. abx019257, Abexxa, 1:400 dilution). The sections were washed with Dako Wash Buffer (10×) (product code S3006, Agilent, USA). The Peroxidase/DAB+ Rabbit/Mouse Dako Real EnVision Detection System was used to visualise the immunohistochemical reaction (product code K8006, Agilent). The microphotographs of the studied sections come from our own research and were subjected to computer-aided image analysis using cellSens software (Olympus Soft Imaging Solution GmbH, Germany) coupled with an optical Olympus BX53 microscope equipped with a ColorView IIIu digital camera (Olympus, Japan)

Fig. 2

Multiple reaction monitoring (MRM) chromatograms for the analysis of an H-AAEILAATPAAVQLR-OH peptide, which is a fragment of canine podocin in urine sediment. The retention time of 3.78 min corresponds to the studied compound. The presence of an additional signal at 3.32 min is associated with the presence of cis-trans isomerism of the proline residues (29)
Multiple reaction monitoring (MRM) chromatograms for the analysis of an H-AAEILAATPAAVQLR-OH peptide, which is a fragment of canine podocin in urine sediment. The retention time of 3.78 min corresponds to the studied compound. The presence of an additional signal at 3.32 min is associated with the presence of cis-trans isomerism of the proline residues (29)
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