<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Introduction:</bold><italic>Mycoplasma synoviae</italic> (MS) is a chicken pathogen of major economic importance.<bold>Material and Methods:</bold> Between 2010 and 2016, 906 commercial layer chicken flocks in Poland were examined for MS, and the phylogenetic relationship among the strains was established. Regionally dispersed samples were collected and tested with the use of real-time PCR to detect the 16S–23S intergenic spacer region. Positive samples were also tested with LAMP and conventional PCR to detect the <italic>vlhA</italic> gene.<bold>Results:</bold> MS genetic material was detected in 265 (29%) of the tested flocks by real-time PCR, in 227 by the LAMP method and in 202 (22%) by conventional PCR. The by-year percentage of positive samples began at 34% in 2010, rose to 44% in 2012, and declined to 29% in 2016. A phylogenetic analysis of Polish <italic>M. synoviae</italic> strains using a partial sequence of the <italic>vlhA</italic> gene showed nine genotypes (A–I), the most frequently occurring being F and C. Pathogenic Polish MS field isolates (n = 27) collected from chickens with clinical signs of infection were grouped for their characteristic symptoms: respiratory for genotypes C, E, F, and I (n = 13), EAA and a drop in laying for genotypes F, E, and C (n = 12), and synovitis for genotype A (n = 2).<bold>Conclusion:</bold> These data showed the country’s isolate diversity. The high prevalence suggests the need to introduce appropriate control programmes. This is the first report of molecular epidemiological data on <italic>M. synoviae</italic> infection in layer chickens in Poland.</abstract>

Introduction:Mycoplasma synoviae (MS) is a chicken pathogen of major economic importance.Material and Methods: Between 2010 and 2016, 906 commercial layer chicken flocks in Poland were examined for MS, and the phylogenetic relationship among the strains was established. Regionally dispersed samples were collected and tested with the use of real-time PCR to detect the 16S–23S intergenic spacer region. Positive samples were also tested with LAMP and conventional PCR to detect the vlhA gene.Results: MS genetic material was detected in 265 (29%) of the tested flocks by real-time PCR, in 227 by the LAMP method and in 202 (22%) by conventional PCR. The by-year percentage of positive samples began at 34% in 2010, rose to 44% in 2012, and declined to 29% in 2016. A phylogenetic analysis of Polish M. synoviae strains using a partial sequence of the vlhA gene showed nine genotypes (A–I), the most frequently occurring being F and C. Pathogenic Polish MS field isolates (n = 27) collected from chickens with clinical signs of infection were grouped for their characteristic symptoms: respiratory for genotypes C, E, F, and I (n = 13), EAA and a drop in laying for genotypes F, E, and C (n = 12), and synovitis for genotype A (n = 2).Conclusion: These data showed the country’s isolate diversity. The high prevalence suggests the need to introduce appropriate control programmes. This is the first report of molecular epidemiological data on M. synoviae infection in layer chickens in Poland.

Prevalence and phylogenetic analysis of <italic>Mycoplasma synoviae</italic> strains isolated from Polish chicken layer flocks

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Prevalence and phylogenetic analysis of  strains isolated from Polish chicken layer flocks

Introduction:Mycoplasma synoviae (MS) is a chicken pathogen of major economic importance.Material and Methods: Between 2010 and 2016, 906 commercial layer...

Prevalence and phylogenetic analysis of Mycoplasma synoviae strains isolated from Polish chicken layer flocks

Online veröffentlicht: 28. März 2019

Seitenbereich: 41 - 49

Eingereicht: 10. Okt. 2018

Akzeptiert: 25. Feb. 2019

DOI: https://doi.org/10.2478/jvetres-2019-0010

Schlüsselwörter
chickens, gene, genotype, pathogenicity

© 2019 Olimpia Kursa et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Prevalence and phylogenetic analysis of Mycoplasma synoviae strains isolated from Polish chicken layer flocks

Online veröffentlicht: 28. März 2019

Seitenbereich: 41 - 49

Eingereicht: 10. Okt. 2018

Akzeptiert: 25. Feb. 2019

DOI: https://doi.org/10.2478/jvetres-2019-0010

Schlüsselwörterchickens, gene, genotype, pathogenicity

© 2019 Olimpia Kursa et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Schlüsselwörter
chickens, gene, genotype, pathogenicity