<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Introduction:</bold> The study aimed to isolate thermophilic <italic>Campylobacter</italic> from chickens raised three rearing methods, determine its antimicrobial susceptibilities, and examine resistance-related genes by PCR.<bold>Material and Methods:</bold> Cloacal swabs or intestinal contents were taken in Istanbul, Sakarya, and Izmir provinces. Chickens were from small village-based family-run businesses (n = 70), organically raised (n = 71), and conventionally raised broilers (n = 79). The samples were cultured on modified charcoal cefoperazone desoxycholate (mCCD) agar. Suspect isolates were identified with multiplex PCR (mPCR). As per EUCAST standards, MIC values were derived by broth microdilution for tetracycline, ciprofloxacin, nalidixic acid, kanamycin, gentamicin, and erythromycin in isolates of <italic>C. jejuni</italic> (n = 98) and <italic>C. coli</italic> (n = 83).<bold>Results:</bold> In <italic>C. jejuni</italic>, 78.6% tetracycline, 87.8% ciprofloxacin, and 81.6% nalidixic acid resistance was detected, but none was to kanamycin, gentamicin, or erythromycin. In <italic>C. coli</italic>, 98.8% ciprofloxacin and 63.9% nalidixic acid resistance was detected, whereas resistance to nonquinolones was not observed. C257T (Thr-86-Ile) mutation in the <italic>gyrA</italic> gene of all phenotypically quinolone-resistant isolates was detected through a mismatch amplification mutation assay PCR (MAMA-PCR). It emerged that all isolates bore the <italic>tet</italic> (O) resistance gene.<bold>Conclusion:</bold> Common tetracycline, nalidixic acid, and ciprofloxacin resistance exists in <italic>Campylobacter</italic> isolated from chickens raised three rearing methods.</abstract>

Introduction: The study aimed to isolate thermophilic Campylobacter from chickens raised three rearing methods, determine its antimicrobial susceptibilities, and examine resistance-related genes by PCR.Material and Methods: Cloacal swabs or intestinal contents were taken in Istanbul, Sakarya, and Izmir provinces. Chickens were from small village-based family-run businesses (n = 70), organically raised (n = 71), and conventionally raised broilers (n = 79). The samples were cultured on modified charcoal cefoperazone desoxycholate (mCCD) agar. Suspect isolates were identified with multiplex PCR (mPCR). As per EUCAST standards, MIC values were derived by broth microdilution for tetracycline, ciprofloxacin, nalidixic acid, kanamycin, gentamicin, and erythromycin in isolates of C. jejuni (n = 98) and C. coli (n = 83).Results: In C. jejuni, 78.6% tetracycline, 87.8% ciprofloxacin, and 81.6% nalidixic acid resistance was detected, but none was to kanamycin, gentamicin, or erythromycin. In C. coli, 98.8% ciprofloxacin and 63.9% nalidixic acid resistance was detected, whereas resistance to nonquinolones was not observed. C257T (Thr-86-Ile) mutation in the gyrA gene of all phenotypically quinolone-resistant isolates was detected through a mismatch amplification mutation assay PCR (MAMA-PCR). It emerged that all isolates bore the tet (O) resistance gene.Conclusion: Common tetracycline, nalidixic acid, and ciprofloxacin resistance exists in Campylobacter isolated from chickens raised three rearing methods.

Phenotypic and genotypic examination of antimicrobial resistance in thermophilic <italic>Campylobacter</italic> species isolated from poultry in Turkey

Department of Microbiology, Faculty of Veterinary Medicine

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Phenotypic and genotypic examination of antimicrobial resistance in thermophilic  species isolated from poultry in Turkey

Introduction: The study aimed to isolate thermophilic Campylobacter from chickens raised three rearing methods, determine its antimicrobial susceptibilities,...

Phenotypic and genotypic examination of antimicrobial resistance in thermophilic Campylobacter species isolated from poultry in Turkey

Online veröffentlicht: 31. Dez. 2018

Seitenbereich: 463 - 468

Eingereicht: 28. Juli 2018

Akzeptiert: 28. Nov. 2018

DOI: https://doi.org/10.2478/jvetres-2018-0071

Schlüsselwörter
chicken, antimicrobial resistance, PCR, stools, thermophilic

© 2018 Mehmet Cemal Adiguzel et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Phenotypic and genotypic examination of antimicrobial resistance in thermophilic Campylobacter species isolated from poultry in Turkey

Online veröffentlicht: 31. Dez. 2018

Seitenbereich: 463 - 468

Eingereicht: 28. Juli 2018

Akzeptiert: 28. Nov. 2018

DOI: https://doi.org/10.2478/jvetres-2018-0071

Schlüsselwörterchicken, antimicrobial resistance, PCR, stools, thermophilic

© 2018 Mehmet Cemal Adiguzel et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Schlüsselwörter
chicken, antimicrobial resistance, PCR, stools, thermophilic