<abstract xmlns="http://www.w3.org/1999/xhtml"><sec id="j_jvetres-2018-0054_s_005_w2aab3b7c21b1b6b1aab1c16b1Aa"><h3>Introduction</h3><p>Intestinal obstruction such as atresia coli causes pathophysiological changes in gastrointestinal tissue due to the rise of intra-abdominal pressure. The aim of this study is to determine the intestinal damage with intestinal biomarkers in calves with atresia coli.</p></sec><sec id="j_jvetres-2018-0054_s_006_w2aab3b7c21b1b6b1aab1c16b2Aa"><h3>Material and Methods</h3><p>The study was conducted on 40 Holstein calves diagnosed with atresia coli with mild to moderate abdominal distention and 10 healthy Holstein calves which served as the control. Blood samples were collected from all calves, and then serum concentrations of intestinal biomarkers were estimated, namely intestinal fatty acid binding protein (IFABP), liver fatty acid binding protein (LFABP), trefoil factor 3 (TFF3), and intestinal alkaline phosphatase (IAP), using commercially available specific bovine ELISA kits. An automatic blood gas analyser was employed for determining the lactate concentration.</p></sec><sec id="j_jvetres-2018-0054_s_007_w2aab3b7c21b1b6b1aab1c16b3Aa"><h3>Results</h3><p>The concentrations of serum LFABP (P &lt; 0.01), IFABP, TFF3, IAP, and blood lactate (P &lt; 0.001) were significantly higher in calves with atresia coli than in healthy calves.</p></sec><sec id="j_jvetres-2018-0054_s_008_w2aab3b7c21b1b6b1aab1c16b4Aa"><h3>Conclusion</h3><p>The calves affected with atresia coli exhibited severe intestinal damage, and IFABP, LFABP, and TFF3 have significant diagnostic importance and play a useful role in determining the intestinal damage due to intestinal obstruction. High levels of IAP and lactate may serve as a signal for the development of intestinal injury.</p></sec></abstract>

IntroductionIntestinal obstruction such as atresia coli causes pathophysiological changes in gastrointestinal tissue due to the rise of intra-abdominal pressure. The aim of this study is to determine the intestinal damage with intestinal biomarkers in calves with atresia coli.Material and MethodsThe study was conducted on 40 Holstein calves diagnosed with atresia coli with mild to moderate abdominal distention and 10 healthy Holstein calves which served as the control. Blood samples were collected from all calves, and then serum concentrations of intestinal biomarkers were estimated, namely intestinal fatty acid binding protein (IFABP), liver fatty acid binding protein (LFABP), trefoil factor 3 (TFF3), and intestinal alkaline phosphatase (IAP), using commercially available specific bovine ELISA kits. An automatic blood gas analyser was employed for determining the lactate concentration.ResultsThe concentrations of serum LFABP (P < 0.01), IFABP, TFF3, IAP, and blood lactate (P < 0.001) were significantly higher in calves with atresia coli than in healthy calves.ConclusionThe calves affected with atresia coli exhibited severe intestinal damage, and IFABP, LFABP, and TFF3 have significant diagnostic importance and play a useful role in determining the intestinal damage due to intestinal obstruction. High levels of IAP and lactate may serve as a signal for the development of intestinal injury.

Evaluation of intestinal damage biomarkers in calves with atresia coli

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"Evaluation of intestinal damage biomarkers in calves with atresia coli"}

IntroductionIntestinal obstruction such as atresia coli causes pathophysiological changes in gastrointestinal tissue due to the rise of intra-abdominal...

Parameters	Healthy calves (n:10)	Calves with atresia coli (n:40)
IFABP (ng/mL)*** Median (min/max)	14.5 (12.4/16.2)	17.1 (12.4/36.2)
LFABP Mean ±SEM (ng/mL)**	3.18 ± 0.3	4.48 ± 0.24
IAP Mean (ng/±SEM mL)***	30.0 ± 1.33	40.7 ± 2.43
TFFMedian 3 (ng/(min/mL)*** max)	1.6 (0.9/1.9)	3.8 (1.5/68)
Lactate (mmol/L)*** Mean ±SEM	1.65 ± 0.09	3.88 ± 0.39
SatO₂ (%)** Mean ±SEM	55.5 ± 6.39	31.9 ± 2.15
WBC Mean (±10SEM 3/μL)**	10.4 ± 0.97	15.2 ± 1.46

Evaluation of intestinal damage biomarkers in calves with atresia coli

Online veröffentlicht: 23. Okt. 2018

Seitenbereich: 379 - 384

Eingereicht: 21. Feb. 2018

Akzeptiert: 23. Aug. 2018

DOI: https://doi.org/10.2478/jvetres-2018-0054

Schlüsselwörter
calves, atresia coli, IFABP, LFABP, TFF3

© 2018 R. Yildiz et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

IFABP, LFABP, IAP, TFF3, lactate, SatO2, and WBC levels in healthy calves and calves with atresia coli

Evaluation of intestinal damage biomarkers in calves with atresia coli

Online veröffentlicht: 23. Okt. 2018

Seitenbereich: 379 - 384

Eingereicht: 21. Feb. 2018

Akzeptiert: 23. Aug. 2018

DOI: https://doi.org/10.2478/jvetres-2018-0054

Schlüsselwörtercalves, atresia coli, IFABP, LFABP, TFF3

© 2018 R. Yildiz et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

IFABP, LFABP, IAP, TFF3, lactate, SatO2, and WBC levels in healthy calves and calves with atresia coli

Schlüsselwörter
calves, atresia coli, IFABP, LFABP, TFF3