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Genetic analysis of Toxocara spp. in stray cats and dogs in Van province, Eastern Turkey

Bekir Oguz

Bekir Oguz

Department of Parasitology, Faculty of Veterinary Medicine, Van Yuzuncu Yil UniversityVan, Turkey
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Oguz, Bekir
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Nalan Ozdal

Nalan Ozdal

Department of Parasitology, Faculty of Veterinary Medicine, Van Yuzuncu Yil UniversityVan, Turkey
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Ozdal, Nalan
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M. Serdar Deger

M. Serdar Deger

Department of Parasitology, Faculty of Veterinary Medicine, Van Yuzuncu Yil UniversityVan, Turkey
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Serdar Deger, M.
  
23. Okt. 2018
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Online veröffentlicht: 23. Okt. 2018
Seitenbereich: 291 - 295
Eingereicht: 29. März 2018
Akzeptiert: 27. Aug. 2018
DOI: https://doi.org/10.2478/jvetres-2018-0042
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© 2018 B. Oguz et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

A – the tail-end of Toxocara canis male, B – the tail-end of Toxocara canis female, C – the cephalic alae of T. canis, D – the cephalic alae of T. cati, E – eggs of Toxocara canis. A, B, C, D – bars indicate 1,000 μm. E – bars indicate 100 μm
A – the tail-end of Toxocara canis male, B – the tail-end of Toxocara canis female, C – the cephalic alae of T. canis, D – the cephalic alae of T. cati, E – eggs of Toxocara canis. A, B, C, D – bars indicate 1,000 μm. E – bars indicate 100 μm

Fig. 2

PCR amplification of ITS-2 (~400 bp) of Toxocara genes on 1.5% agarose gel. M – 50 bp DNA marker, N – negative control. Samples from 1 to 7 represent Toxocara canis; samples from 8 to 10 represent Toxocara cati
PCR amplification of ITS-2 (~400 bp) of Toxocara genes on 1.5% agarose gel. M – 50 bp DNA marker, N – negative control. Samples from 1 to 7 represent Toxocara canis; samples from 8 to 10 represent Toxocara cati

Fig. 3

Neighbour-joining phylogenetic tree (1,000 bootstraps) based on the ITS-2 sequence of ribosomal DNA of T. canis and T. cati isolated from cats and dogs (marked brown) and other sequences of ascarid retrieved from GenBank. The sequence of Ascaridia galli ITS-2 was used as the out-group
Neighbour-joining phylogenetic tree (1,000 bootstraps) based on the ITS-2 sequence of ribosomal DNA of T. canis and T. cati isolated from cats and dogs (marked brown) and other sequences of ascarid retrieved from GenBank. The sequence of Ascaridia galli ITS-2 was used as the out-group
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