<abstract xmlns="http://www.w3.org/1999/xhtml"><sec id="j_jvetres-2018-0025_s_005_w2aab3b7b8b1b6b1aab1c16b1Aa"><h3>Introduction</h3><p>The major difficulty in analysis of nitrofurans in feed, feed water, and food of animal origin is that nitrofurans have low molecular weights and fast metabolism. The principal goal of this study was to prepare a procedure for the determination of nitrofurans and their metabolites by a single method in different types of feed, feed water, and food of animal origin.</p></sec><sec id="j_jvetres-2018-0025_s_006_w2aab3b7b8b1b6b1aab1c16b2Aa"><h3>Material and Methods</h3><p>Two-gram samples were subjected to hydrolysis and derivatisation processes by addition of hydrochloric acid and 2-nitrobenzaldehyde. After incubation the sample was purified by solid phase extraction technique. Nitrofurans were analysed using ultra-high-pressure liquid chromatography-MS/MS (UHPLC-MS/MS).</p></sec><sec id="j_jvetres-2018-0025_s_007_w2aab3b7b8b1b6b1aab1c16b3Aa"><h3>Results</h3><p>The results of validation fulfil the requirement of the confirmatory criteria according to the European Commission Decision 2002/657/EC regarding apparent recoveries (88.9%–107.3%), repeatability (2.9%–9.4%) and within-laboratory reproducibility (4.4%–10.7%).</p></sec><sec id="j_jvetres-2018-0025_s_008_w2aab3b7b8b1b6b1aab1c16b4Aa"><h3>Conclusion</h3><p>The method can be successfully applied to monitor nitrofurans and their metabolites in different matrices.</p></sec></abstract>

IntroductionThe major difficulty in analysis of nitrofurans in feed, feed water, and food of animal origin is that nitrofurans have low molecular weights and fast metabolism. The principal goal of this study was to prepare a procedure for the determination of nitrofurans and their metabolites by a single method in different types of feed, feed water, and food of animal origin.Material and MethodsTwo-gram samples were subjected to hydrolysis and derivatisation processes by addition of hydrochloric acid and 2-nitrobenzaldehyde. After incubation the sample was purified by solid phase extraction technique. Nitrofurans were analysed using ultra-high-pressure liquid chromatography-MS/MS (UHPLC-MS/MS).ResultsThe results of validation fulfil the requirement of the confirmatory criteria according to the European Commission Decision 2002/657/EC regarding apparent recoveries (88.9%–107.3%), repeatability (2.9%–9.4%) and within-laboratory reproducibility (4.4%–10.7%).ConclusionThe method can be successfully applied to monitor nitrofurans and their metabolites in different matrices.

New method of analysis of nitrofurans and nitrofuran metabolites in different biological matrices using UHPLC-MS/MS

Department of Pharmacology and Toxicology, National Veterinary Research Institute

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

IntroductionThe major difficulty in analysis of nitrofurans in feed, feed water, and food of animal origin is that nitrofurans have low molecular weights and...

New method of analysis of nitrofurans and nitrofuran metabolites in different biological matrices using UHPLC-MS/MS

Online veröffentlicht: 07. Juli 2018

Seitenbereich: 161 - 166

Eingereicht: 21. Feb. 2018

Akzeptiert: 11. Juni 2018

DOI: https://doi.org/10.2478/jvetres-2018-0025

Schlüsselwörter
nitrofurans, feed water, feed, food products, UHPLC-MS/MS

© 2018 Tomasz Śniegocki et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

New method of analysis of nitrofurans and nitrofuran metabolites in different biological matrices using UHPLC-MS/MS

Online veröffentlicht: 07. Juli 2018

Seitenbereich: 161 - 166

Eingereicht: 21. Feb. 2018

Akzeptiert: 11. Juni 2018

DOI: https://doi.org/10.2478/jvetres-2018-0025

Schlüsselwörternitrofurans, feed water, feed, food products, UHPLC-MS/MS

© 2018 Tomasz Śniegocki et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Schlüsselwörter
nitrofurans, feed water, feed, food products, UHPLC-MS/MS