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Apparent Vmax, Km and Hill coefficient h of ACC oxidase from Callistephus chinensis seeds analyzed in vivo and in vitro at 25 °C
ACC oxidase activity
Vmax (nL C2H4·mg protein−1·h−1) ± SE
Km for ACC (μM) ± SE
Hill coefficient h ± SE
In vivo
10.89 ± 1.13
368 ± 92
0.63 ± 0.097
In vitro
2.299 ± 0.14
154 ± 20
1.73 ± 0.39
Effect of PVPP and NaHCO3 on in vitro activity of ACC oxidase from Callistephus chinensis seeds germinated for 48 h. Means followed with the same letter are not significantly different at p = 0.05 according to Duncan's test; mean ± SE