Effect of Different Soils on Pheromone-Enhanced Movement of Entomopathogenic Nematodes

Entomopathogenic nematodes (EPNs) have a specialized infective juvenile stage (IJ) that is mobile and has the capability to seek insect hosts to penetrate their haemocoel. EPNs are primarily applied to soil as biological control agents; thus, the IJs must move through the soil to find and infect a host. Soil characteristics are known to be an important factor that can affect the efficiency of EPN movement behavior. Previous research has shown that exposure to ascaroside pheromones can enhance EPN movement and infectivity in soil. The ability of pheromones to enhance EPN efficacy was recently demonstrated under field conditions in a pecan orchard. However, prior to our research, it was unknown whether different soils have differential effects on pheromone enhanced EPN efficacy. In different soils, we tested the biocontrol efficacy of Steinernema carpocapsae, Steinernema feltiae and Heterorhabditis bacteriophora in soil columns with and without pheromone exposure. All nematodes were evaluated in separate columns filled with oven dried commercial play sand and two different soils from pecan orchards (from Byron, GA and Tifton, GA). The soils differed substantially in several aspects such as field capacity, organic matter, nutrients, and nematode movement capacity. Efficacy was determined by baiting the bottom section of each column with larvae of the yellow mealworm (Tenebrio molitor L.). Results indicated that pheromones enhanced EPN efficacy for all EPN species and soils tested compared to treatments without pheromones. The magnitude/extent that pheromones boosted EPN movement in all EPNs regardless of soil type did not differ. Soil did not affect EPN efficacy for H. bacteriophora but did affect S. carpocapsae and S. feltiae. For both S. carpocapsae and S. feltiae efficacy was highest in the sandy field soil (Tifton soil) followed by that of the loamy sand (Byron soil) and pure sand (commercial play sand). When comparing the efficacy of EPN species to each other, we observed that H. bacteriophora killed more bait insects exposed to soil in the bottom of the soil column than other EPNs. Our findings suggest that pheromones can be used to enhance EPN efficacy in diverse soils. Future research may explore pheromone effects on EPNs in additional substrates.