<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Specimens of a tylenchid nematode were recovered in 2019 from soil samples collected from a corn field, located in Pickens County, South Carolina, USA. A moderate number of <italic>Tylenchus</italic> sp. adults (females and males) were recovered. Extracted nematodes were examined morphologically and molecularly for species identification, which indicated that the specimens of the tylenchid adults were a new species, described herein as <italic>Tylenchus zeae</italic> n. sp. Morphological examination and the morphometric details of the specimens were very close to the original descriptions of <italic>Tylenchus sherianus</italic> and <italic>T. rex</italic>. However, females of the new species can be differentiated from these species by body shape and length, shape of excretory duct, distance between anterior end and esophageal intestinal valve, and a few other characteristics given in the diagnosis. Males of the new species can be differentiated from the two closely related species by tail, spicules, and gubernaculum length. Cryo-scanning electron microscopy confirmed head bearing five or six annules; four to six cephalic sensilla represented by small pits at the rounded corners of the labial plate; a small, round oral plate; and a large, pit-like amphidial opening confined to the labial plate and extending three to four annules beyond it. Phylogenetic analysis of 18S rRNA gene sequences placed <italic>Tylenchus zeae</italic> n. sp. in a clade with <italic>Tylenchus arcuatus</italic> and several <italic>Filenchus</italic> spp., and the mitochondrial cytochrome oxidase c subunit 1 (<italic>COI</italic>) gene region separated the new species from <italic>T. arcuatus</italic> and other tylenchid species. In the 28S tree, <italic>T. zeae</italic> n. sp. showed a high level of sequence divergence and was positioned outside of the main <italic>Tylenchus-Filenchus</italic> clade.</p></abstract>

Specimens of a tylenchid nematode were recovered in 2019 from soil samples collected from a corn field, located in Pickens County, South Carolina, USA. A moderate number of Tylenchus sp. adults (females and males) were recovered. Extracted nematodes were examined morphologically and molecularly for species identification, which indicated that the specimens of the tylenchid adults were a new species, described herein as Tylenchus zeae n. sp. Morphological examination and the morphometric details of the specimens were very close to the original descriptions of Tylenchus sherianus and T. rex. However, females of the new species can be differentiated from these species by body shape and length, shape of excretory duct, distance between anterior end and esophageal intestinal valve, and a few other characteristics given in the diagnosis. Males of the new species can be differentiated from the two closely related species by tail, spicules, and gubernaculum length. Cryo-scanning electron microscopy confirmed head bearing five or six annules; four to six cephalic sensilla represented by small pits at the rounded corners of the labial plate; a small, round oral plate; and a large, pit-like amphidial opening confined to the labial plate and extending three to four annules beyond it. Phylogenetic analysis of 18S rRNA gene sequences placed Tylenchus zeae n. sp. in a clade with Tylenchus arcuatus and several Filenchus spp., and the mitochondrial cytochrome oxidase c subunit 1 (COI) gene region separated the new species from T. arcuatus and other tylenchid species. In the 28S tree, T. zeae n. sp. showed a high level of sequence divergence and was positioned outside of the main Tylenchus-Filenchus clade.

Molecular and morphological characterization of <italic>Tylenchus zeae</italic> n. sp. (Nematoda: Tylenchida) from Corn (<italic>Zea mays</italic>) in South Carolina

Journal of Nematology

This work is licensed under the Creative Commons Attribution 4.0 International License.

Molecular and morphological characterization of  n. sp. (Nematoda: Tylenchida) from Corn () in South Carolina

Specimens of a tylenchid nematode were recovered in 2019 from soil samples collected from a corn field, located in Pickens County, South Carolina, USA. A...

Molecular and morphological characterization of Tylenchus zeae n. sp. (Nematoda: Tylenchida) from Corn (Zea mays) in South Carolina

Article Category: Research Paper

Online veröffentlicht: 28. Feb. 2023

Seitenbereich: -

Eingereicht: 12. Mai 2022

DOI: https://doi.org/10.2478/jofnem-2023-0003

Schlüsselwörter
18S, 28S, Corn, DNA sequencing, Molecular phylogeny, Morphology, Nematode, New species, Taxonomy, n. sp.

© 2023 Kantor et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Molecular and morphological characterization of Tylenchus zeae n. sp. (Nematoda: Tylenchida) from Corn (Zea mays) in South Carolina

Article Category: Research Paper

Online veröffentlicht: 28. Feb. 2023

Seitenbereich: -

Eingereicht: 12. Mai 2022

DOI: https://doi.org/10.2478/jofnem-2023-0003

Schlüsselwörter18S, 28S, Corn, DNA sequencing, Molecular phylogeny, Morphology, Nematode, New species, Taxonomy, n. sp.

© 2023 Kantor et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Schlüsselwörter
18S, 28S, Corn, DNA sequencing, Molecular phylogeny, Morphology, Nematode, New species, Taxonomy, n. sp.