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Morpho-Molecular and Ultrastructural Characterization of Discocriconemella parasinensis n. sp. from Zhejiang Province, China

Junxia Li
Junxia Li
, 
Maria Munawar
Maria Munawar
, 
Pablo Castillo
Pablo Castillo
 und 
Jingwu Zheng
Jingwu Zheng
   | 18. Mai 2022
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Article Category: Research Paper
Online veröffentlicht: 18. Mai 2022
Seitenbereich: -
Eingereicht: 09. Sept. 2021
DOI: https://doi.org/10.2478/jofnem-2022-0011
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© 2022 Junxia Li et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1

Line drawings of D. parasinensis n. sp. Female. (A) Esophageal region; (B, C) En face view of lip region; (D) Gonad; (E–G) Posterior region showing position of vulva and anus (Scale bars: A–C, E–G = 10 μm, D = 50 μm).
Line drawings of D. parasinensis n. sp. Female. (A) Esophageal region; (B, C) En face view of lip region; (D) Gonad; (E–G) Posterior region showing position of vulva and anus (Scale bars: A–C, E–G = 10 μm, D = 50 μm).

Figure 2

Light photomicrographs of D. parasinensis n. sp. Female. (A) Entire body, arrowhead indicating position of vulva (v = vulva); (B, C) Esophageal region arrowheads indicating position of base of esophageal bulb (ph.b) and excretory pore (exp); (D) Posterior region showing entire gonad arrowheads indicating position of vulva (v) and spermatheca (sp); (E) Mid-body (arrowhead pointing anastomoses); (F) Vulval region arrowhead showing spermatheca (sp); (G–I) Tail region, arrowheads showing position of vulva (v) and anus (a) (Scale bars: A = 100 μm, B–D = 50 μm, E–I = 10 μm).
Light photomicrographs of D. parasinensis n. sp. Female. (A) Entire body, arrowhead indicating position of vulva (v = vulva); (B, C) Esophageal region arrowheads indicating position of base of esophageal bulb (ph.b) and excretory pore (exp); (D) Posterior region showing entire gonad arrowheads indicating position of vulva (v) and spermatheca (sp); (E) Mid-body (arrowhead pointing anastomoses); (F) Vulval region arrowhead showing spermatheca (sp); (G–I) Tail region, arrowheads showing position of vulva (v) and anus (a) (Scale bars: A = 100 μm, B–D = 50 μm, E–I = 10 μm).

Figure 3

Scanning electron micrographs of D. parasinensis n. sp. Female. (A) Entire body, arrowhead indicating position of vulva (v = vulva); (B–F) Lip regions; (G) Cuticular annuli at mid-body; (H–J) Tail region, arrowheads showing position of vulva (v) and anus (a) (Scale bars: A = 50 μm; B–J = 10 μm).
Scanning electron micrographs of D. parasinensis n. sp. Female. (A) Entire body, arrowhead indicating position of vulva (v = vulva); (B–F) Lip regions; (G) Cuticular annuli at mid-body; (H–J) Tail region, arrowheads showing position of vulva (v) and anus (a) (Scale bars: A = 50 μm; B–J = 10 μm).

Figure 4

Phylogenetic relationships of D. parasinensis n. sp. with other criconematids species as inferred from Bayesian analysis using the 18S rRNA gene sequence dataset with the TIM1+I+G model. Posterior probability >70% is given for appropriate clades. Newly obtained sequences are indicated in bold.
Phylogenetic relationships of D. parasinensis n. sp. with other criconematids species as inferred from Bayesian analysis using the 18S rRNA gene sequence dataset with the TIM1+I+G model. Posterior probability >70% is given for appropriate clades. Newly obtained sequences are indicated in bold.

Figure 5

Phylogenetic relationships of D. parasinensis n. sp. with other criconematids species as inferred from Bayesian analysis using the D2–D3 of 28S rRNA gene sequence dataset with the TIM3+I +G model. Posterior probability >70% is given for appropriate clades. Newly obtained sequences are indicated in bold.
Phylogenetic relationships of D. parasinensis n. sp. with other criconematids species as inferred from Bayesian analysis using the D2–D3 of 28S rRNA gene sequence dataset with the TIM3+I +G model. Posterior probability >70% is given for appropriate clades. Newly obtained sequences are indicated in bold.

Figure 6

Phylogenetic relationships of D. parasinensis n. sp. with other criconematids species as inferred from Bayesian analysis using the ITS rRNA gene sequence dataset with the GTR+I+G model. Posterior probability >70% is given for appropriate clades. Newly obtained sequences are indicated in bold.
Phylogenetic relationships of D. parasinensis n. sp. with other criconematids species as inferred from Bayesian analysis using the ITS rRNA gene sequence dataset with the GTR+I+G model. Posterior probability >70% is given for appropriate clades. Newly obtained sequences are indicated in bold.

Morphometric data of female of D. parasinensis n. sp.

Character Holotype Paratype
n – 15
L 477.0 486.1 ± 37.6 (382–556)
a 7.8 7.7 ± 0.5 (6.7–9.0)
b 4.0 4.3 ± 0.3 (3.6–4.6)
c 15.5 16.4 ± 2.0 (13.8–20.6)
c′ 1.1 1.0 ± 0.1 (0.9–1.3)
V 89.6 89.6 ± 0.8 (88.0–91.0)
VL/VB 1.3 1.2 ± 0.1 (1.1–1.5)
R 82 82.6 ± 1.9 (80–86)
Rex 22 22.0 ± 0.7 (21.0–23.0)
RV 10 10.1 ± 0.4 (9–11)
RVan 3 2.9 ± 0.2 (2–3)
Ran 6 6.1 ± 0.3 (6–7)
Lip height 9.0 7.8 ± 0.6 (7.0–9.0)
Lip diam. 19.0 20.0 ± 1.0 (18.0–22.0)
Stylet length 68.0 68.3 ± 4.9 (59.0–76.0)
Stylet (% L) 14.3 14.1 ± 1.3 (12.3–16.8)
Pharynx 119.0 114.0 ± 5.8 (103.0–123.0)
Max. Body diam. 61.0 63.2 ± 6.3 (51.0–72.0)
Vulval body diam. 38.0 40.9 ± 3.6 (33.0–47.0)
Vulva to tail terminus 49.0 50.4 ± 4.4 (41.0–56.0)
Anal body diam. 28.0 30.0 ± 3.5 (24.0–35.0)
Tail length 31.0 30.0 ± 3.9 (23.0–36.0)
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