<abstract xmlns="http://www.w3.org/1999/xhtml">

Electrical impedance spectroscopy is a well-established tool for monitoring changes in the electrical properties of tissue. Most tissue and organ types have been investigated in various studies. As for the small intestine, there are several published studies conducted on pig and rat models. This study investigates the changes in passive electrical properties of the complete wall of the human intestine non-invasively during ischemia. We aim to use the passive electrical properties to assess intestinal viability. The bioimpedance measurements were performed using a two-electrode set-up with a Solartron 1260 Impedance/gain-phase analyser. The small intestinal samples were resected from patients who underwent pancreaticoduodenectomy. Impedance measurements were conducted following resection by placing the electrodes on the surface of the intestine. A voltage was applied across the intestinal sample and the measured electrical impedance was obtained in the ZPlot software. Impedance data were further fitted into a Cole model to obtain the Cole parameters. The <italic>Py</italic> value was calculated from the extracted Cole parameters and used to assess the cell membrane integrity, thus evaluate the intestinal viability. Eight small intestinal segments from different patients were used in this study and impedance measurements were performed once an hour for a ten-hour period. One hour after resection, the impedance decreased, then increased the next two hours, before decreasing until the end of the experiment. For all the intestinal segments, the <italic>Py</italic> values first increased and reached a plateau which lasted for 1 - 2 hours, before it decreased irreversibly. The time interval where <italic>Py</italic> value reached the maximum is consistent with reported viable/non-viable limits from histological analysis.</abstract>

Electrical impedance spectroscopy is a well-established tool for monitoring changes in the electrical properties of tissue. Most tissue and organ types have been investigated in various studies. As for the small intestine, there are several published studies conducted on pig and rat models. This study investigates the changes in passive electrical properties of the complete wall of the human intestine non-invasively during ischemia. We aim to use the passive electrical properties to assess intestinal viability. The bioimpedance measurements were performed using a two-electrode set-up with a Solartron 1260 Impedance/gain-phase analyser. The small intestinal samples were resected from patients who underwent pancreaticoduodenectomy. Impedance measurements were conducted following resection by placing the electrodes on the surface of the intestine. A voltage was applied across the intestinal sample and the measured electrical impedance was obtained in the ZPlot software. Impedance data were further fitted into a Cole model to obtain the Cole parameters. The Py value was calculated from the extracted Cole parameters and used to assess the cell membrane integrity, thus evaluate the intestinal viability. Eight small intestinal segments from different patients were used in this study and impedance measurements were performed once an hour for a ten-hour period. One hour after resection, the impedance decreased, then increased the next two hours, before decreasing until the end of the experiment. For all the intestinal segments, the Py values first increased and reached a plateau which lasted for 1 - 2 hours, before it decreased irreversibly. The time interval where Py value reached the maximum is consistent with reported viable/non-viable limits from histological analysis.

Assessing ischemic injury in human intestine ex vivo with electrical impedance spectroscopy

Journal of Electrical Bioimpedance

This work is licensed under the Creative Commons Attribution 4.0 International License.

Electrical impedance spectroscopy is a well-established tool for monitoring changes in the electrical properties of tissue. Most tissue and organ types have...

Assessing ischemic injury in human intestine ex vivo with electrical impedance spectroscopy

Online veröffentlicht: 29. Nov. 2021

Seitenbereich: 82 - 88

Eingereicht: 30. Sept. 2021

DOI: https://doi.org/10.2478/joeb-2021-0011

Schlüsselwörter
Bioimpedance, Human small intestine, Cole model, value

© 2021 Jie Hou, Runar Strand-Amundsen, Stina Hødnebø, Tor Inge Tønnessen, and Jan Olav Høgetveit, published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1

Figure 2

Figure 3

Figure 4

Assessing ischemic injury in human intestine ex vivo with electrical impedance spectroscopy

Online veröffentlicht: 29. Nov. 2021

Seitenbereich: 82 - 88

Eingereicht: 30. Sept. 2021

DOI: https://doi.org/10.2478/joeb-2021-0011

SchlüsselwörterBioimpedance, Human small intestine, Cole model, value

© 2021 Jie Hou, Runar Strand-Amundsen, Stina Hødnebø, Tor Inge Tønnessen, and Jan Olav Høgetveit, published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1

Figure 2

Figure 3

Figure 4

Schlüsselwörter
Bioimpedance, Human small intestine, Cole model, value